Navigation Links
Purdue researchers obtain a snapshot clarifying how materials enter cells
Date:11/30/2007

rystallography to obtain a picture of a special protein, called an ABC transporter protein, as it moved material through the cellular membrane. The work was published in last week's issue of Nature.

In addition to Davidson, Chen's research team includes postdoctoral research associates Michael Oldham and Dheeraj Khare from Purdue, and professor Florante Quiocho from Baylor College of Medicine. The team began this work in 2001.

ABC proteins are present in every living thing and have important biological functions. Scientists have identified 49 different ABC proteins in humans and have found that more than a dozen disease states are associated with malfunctions of these proteins, including macular dystrophy and problems in the regulation of cholesterol and insulin secretion.

Chen's team isolated ABC proteins from an E. coli bacterium, which is the standard research subject for this field of work. The ABC proteins are structurally very similar to those in human cells, and most of the principles can be directly applied to humans, Chen said.

Membrane proteins are notoriously difficult to study, Chen said. While most proteins dissolve in water and can be easily crystallized and examined, membrane proteins dissolve only in fatty substances, making it hard to isolate them for study.

The collaboration between Chen and Davidson, a structural biologist and a biochemist, was the key to success in capturing the intermediate structure, Chen said.

Davidson identified a special mutant of the ABC protein that locks halfway through the process. The mutant trapped the protein complex in a stable form that allowed Chen to crystallize and visualize the structure.

"We have the common goal to learn how this family of proteins works, but we take different approaches," Chen said. "It is important to communicate with scientists in other fields who can offer clues and tools to help reach your goal. We used genetic data and bio
'/>"/>

Contact: Elizabeth K. Gardner
ekgardner@purdue.edu
765-494-2081
Purdue University  
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Purdue researchers click nutrition with camera diet study
2. Purdue research finds similarities in dog, human breast cancer pre-malignant lesions
3. Researchers identify proteins involved in new neurodegenerative syndrome
4. Texas researchers and educators head for Antarctica
5. MGH researchers describe new way to identify, evolve novel enzymes
6. University of Pennsylvania researchers develop formula to gauge risk of disease clusters
7. U of MN researchers discover noninvasive diagnostic tool for brain diseases
8. U of Minnesota researchers discover noninvasive diagnostic tool for brain diseases
9. Researchers discover new strategies for antibiotic resistance
10. Researchers find new taste in fruit flies: carbonated water
11. Binghamton University researchers investigate evolving malaria resistance
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Purdue researchers obtain a snapshot clarifying how materials enter cells
(Date:12/11/2014)... , Dec. 10, 2014  That blood pressure plays a ... while. Hypertension – the medical term for high blood pressure ... 1800s, and the inflatable cuff that,s used in measuring blood ... mean there,s nothing new about hypertension, its triggers and its ...
(Date:12/10/2014)... 2014 You,ve been here before: you desperately need to sign ... key or the answer to your secret question. What,s your dog,s ... Hoyos Labs , a digital infrastructure security company, launches ... frustration that comes with usernames, passwords and PINs – 1U ...
(Date:12/10/2014)... WINSTON-SALEM, N.C. , Dec. 9, 2014  Wake Forest Baptist ... building for its School of Medicine. Funding for this $50 ... that will be publicly launched next summer. ... 60 series R.J. Reynolds Tobacco Company complex, adjacent to 525@vine ...
Breaking Biology News(10 mins):Research points to need for new approaches to treatment of high blood pressure 2Research points to need for new approaches to treatment of high blood pressure 3Research points to need for new approaches to treatment of high blood pressure 4The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 4
... field of schizophrenia research has come alive with many ... introduction of chlorpromazine to the current day, all treatments ... had, at their core, a single treatment mechanism, the ... of clozapine in the 1980s suggested a potential that ...
... Loss of a small portion of chromosome 16, known ... from the University of Chicago Medical Center, the University ... Institute in an article, published Dec. 21, 2007, online ... this genetic "microdeletion" occurred in only four out of ...
... Dr. McWhorter Joins Olive, Edwards & Cooper with Technical, Products ... ... Edwards & Cooper,announced the addition of Dr. David McWhorter as a ... expand into the,homeland security marketplace. His immediate focus will be ensuring ...
Cached Biology News:New treatment mechanisms for schizophrenia 2New treatment mechanisms for schizophrenia 3Recurrent genetic deletion linked to autism 2Chemist Brings SAFETY Act Expertise to Homeland Security Firm 2
(Date:1/22/2015)... (PRWEB) January 22, 2015 Diagenode, ... such as the Bioruptor® and complete solutions for ... for chromatin immunoprecipitation, alleviating the need for manual ... controls needed for ChIP of histones or transcription ...
(Date:1/22/2015)... , Jan. 22, 2015   Cypher Genomics, Inc., ... Inc. (NASDAQ: SQNM ), the leading ... for next generation noninvasive prenatal tests (NIPT). Through ... technology, called Mantis™, to advance analysis of clinically-relevant ...
(Date:1/22/2015)... , Jan. 22, 2015   GenoSpace , a precision ... tools to enable the broad use of genomic, imaging and ... the appointment of Michelle Munson , CEO of Aspera, ... Logo - http://photos.prnewswire.com/prnh/20150122/170713 ...
(Date:12/25/2014)... 25, 2014 The report “Service Quality ... Type & Provider Type - Global Advancements, Worldwide Forecasts ... an in-depth analysis and forecasting of revenues. , ... 221 pages and in-depth TOC on “Service Quality Management ...
Breaking Biology Technology:Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2Cypher Genomics and Sequenom Announce Development Agreement 2Cypher Genomics and Sequenom Announce Development Agreement 3Cypher Genomics and Sequenom Announce Development Agreement 4GenoSpace Expands Board with Appointment of Michelle Munson 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 3Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 4
... WASHINGTON, Oct. 1 Today a promising young,scholar ... Mexico, accepted,the Manuel Velasco-Suarez Award in Bioethics for ... field of bioethics in Latin America during,the 27th ... Naftolin, a trustee of the Pan American Health ...
... Quarter 2007 Earnings Conference Call Thursday, November 8, 2007, ... 5:00 p.m. U.S. EST, FRAZER, Pa., Oct. 1 ... for 5:00 p.m. EST on Thursday, November 8, 2007. ... November 8, 2007 Schedule, 4:30 p.m. EST Q3 2007 results ...
... Oct. 1 A highly sensitive, easy-to-use,test that ... as the Best New Veterinary Product for Diagnostics ... time PCR test was selected for its,excellence and ... the global community of animal sciences practitioners and,professionals. ...
Cached Biology Technology:Prestigious Bioethics Award for Scholar from Mexico 2BoVir(R) Named Best New Veterinary Diagnostics Product in International Animal Pharm Awards 2BoVir(R) Named Best New Veterinary Diagnostics Product in International Animal Pharm Awards 3
... Purification Plates are designed for quick and ... 96-well plates feature two 0.25mm holes laser ... well. The wells remain liquid-tight during incubation ... which time the protein diffuses from the ...
... western blots ,• Optically clear plastic construction ... western blots. The reduced size (60 x ... tray requires less dye and antibodies resulting ... user. Pour spouts in each corner facilitate ...
... many sophisticated DNA analysis and protein analysis tasks, ... another sequence analysis product. Imagine having the ability ... be performed each time you have a new ... specify exactly how your output will look for ...
These stopping screens are for the biolistic PDS-1000 He and Hepta systems. Supplied as 500 screens....
Biology Products: