Navigation Links
Proteomics study yields clues as to how tuberculosis might be thwarting the immune system
Date:11/5/2008

BERKELEY, CA -- A link between the immune system and the self-cleaning system by which biological cells rid themselves of obsolete or toxic parts may one day yield new weapons in the fight against tuberculosis and other deadly infectious diseases. Scientists with the U.S. Department of Energy's (DOE) Lawrence Berkeley National Laboratory (Berkeley Lab) have discovered proteins residing in both systems that point to "cross-talk" between them.

In a collaboration between the research groups of Carolyn Bertozzi, director of Berkeley Lab's Molecular Foundry nanoscience center, and Jay Keasling, director of Berkeley Lab's Physical Biosciences Division, profiles were obtained for 546 different types of proteins in the membrane of a phagosome, an organelle of macrophages (a type of white blood cell) that essentially "eats" and destroys invading organisms (a process called phagocytosis). This represents the most comprehensive proteomic analysis of a phagosomal membrane to date.

"We were able to identify many new proteins that were not previously known to reside in the phagosome," said Wenqing Shui, a member of both the Bertozzi and Keasling research groups, and a proteomics specialist who was the lead author on a paper reporting these results in the Proceedings of the National Academy of Sciences.

"One of the new proteins exclusively found in our study, LC3-II, is considered a marker of autophagy, the process that enables cells to clean up their own cytoplasm," Shui said. "Not only was LC3-II present in the phagosome, its level was increased upon the induction of autophagy in macrophages, and reduced when autophagy was suppressed. This indicates cross-talking between autophagy and phagocytosis that may play an important role in the response of the immune system."

The PNAS paper is entitled: "Membrane proteomics of phagosomes suggests a connection to autophagy." Co-authoring this paper in addition to Shui, Bertozzi and Keasling were Leslie Sheu, Jun Liuc, Brian Smart, Christopher Petzold, Tsung-yen Hsieh and Austin Pitcher. Bertozzi and Keasling are also professors at the University of California at Berkeley. In addition, Bertozzi is an investigator with the Howard Hughes Medical Institute, and Keasling is director of the Joint BioEnergy Institute.

When bacteria or other foreign particles invade the body, the first line of defense are the macrophages, which engulf and contain the invaders within the membrane-bound shells of their phagosomes. Once safely contained, the invaders can be killed with digestive enzymes from another cell organelle, called a lysosome, which fuses with the phagosome. Macrophages, like other kinds of cells, also use lysosomal enzymes for internal housekeeping. However, until now there has been no direct biochemical evidence of a link between phagocytosis and autophagy.

Working with latex bead-containing phagosomes isolated in cell lines from mice, Shui and her colleagues performed a detailed analysis of the protein contents of the phagosomal membrane. Unlike earlier proteomic studies, which profiled the entire organelle and focused on abundant water-soluble protein species, the study by the Bertozzi-Keasling groups was membrane-specific and included hydrophobic protein species that are present in the membrane in relatively low amounts.

"We were able to demonstrate the endogenous level of LC3-II in macrophage phagosomes through the combination of sensitive proteomic techniques and biochemical assays," said Shui. "This is an excellent show-case of how a non-biased high-throughput proteomic study can shed new light on the diverse functions and pathways an organelle may engage in."

The LC3-II protein is a critical component of the autophagy machinery and the discovery that the level of its presence in phagosomes is modulated by autophagic activity (along with several other newly identified phagosome proteins not previously associated with autophagy), points to autophagy playing a heretofore unknown role in immune response, particularly against intracellular pathogens such as mycobacterium tuberculosis.

As Shui explained, "After mycobacterium tuberculosis are phagocytosed into the macrophage cell, they are able to subvert various host defense mechanisms, including the killing of bacilli in the phagosome, and survive well inside the cell. Given that induced autophagy activity appears to enhance mycobacterial killing in the phagosome (from a study by the University of New Mexico's Vojo Deretic and his research group), we speculate that mycobacteria may produce specific factors to counteract the bactericidal effect of autophagy activation."

The Bertozzi and Keasling research groups are now examining whether certain mycobacterial products can modulate macrophage autophagy activity. They are also looking for proteins that could specifically mediate the autophagy as well as the phagosome maturation process.

Said Shui, "We might be able to open new avenues for pharmacologic intervention of tuberculosis as well as other infectious diseases."


'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Related biology news :

1. Story ideas from Molecular & Cellular Proteomics
2. Stowers Proteomics Center devises method for assigning probabilities to human protein interactions
3. Powerful yet reliable proteomics techniques are the focus of a new methods book
4. Human Genomics and Proteomics
5. Bridging public and private research on bioinformatics and proteomics
6. Childhood obesity indicates greater risk of school absenteeism, Penn study reveals
7. A study by the MUHC and McGill University opens a new door to understanding cancer
8. Study begins to reveal clues to the cause and progression of sepsis
9. Clones on task serve greater good, evolutionary study shows
10. New study warns limited carbon market puts 20 percent of tropical forest at risk
11. New study examines how rearing environment can alter navigation
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Proteomics study yields clues as to how tuberculosis might be thwarting the immune system
(Date:5/3/2016)... , May 3, 2016  Neurotechnology, a ... the MegaMatcher Automated Biometric Identification System (ABIS) ... large-scale multi-biometric projects. MegaMatcher ABIS can process multiple ... using any combination of fingerprint, face or iris ... MegaMatcher SDK and MegaMatcher Accelerator , ...
(Date:4/19/2016)... DUBAI , UAE, April 20, 2016 ... can be implemented as a compact web-based "all-in-one" system ... in the biometric fingerprint reader or the door interface ... requirements of modern access control systems. The minimal dimensions ... the ID readers into the building installations offer considerable ...
(Date:3/31/2016)... , March 31, 2016 ... ) ("LegacyXChange" or the "Company") LegacyXChange is ... users of its soon to be launched online site ... https://www.youtube.com/channel/UCyTLBzmZogV1y2D6bDkBX5g ) will also provide potential shareholders ... of DNA technology to an industry that is notorious ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... ... 27, 2016 , ... Newly created 4Sight Medical Solutions ... the healthcare market. The company's primary focus is on new product introductions, to ... that are necessary to help companies efficiently bring their products to market. , ...
(Date:6/24/2016)... TOKYO , June 24, 2016  Regular discussions on ... to take place between the two entities said Poloz. ... in Ottawa , he pointed to the ... and the federal government. ... Poloz said, "Both institutions have common economic goals, why not ...
(Date:6/23/2016)... ... June 23, 2016 , ... Mosio, a ... eBook, “Clinical Trials Patient Recruitment and Retention Tips.” Partnering with experienced clinical research ... by providing practical tips, tools, and strategies for clinical researchers. , “The landscape ...
(Date:6/23/2016)... A person commits a crime, and the detective ... the criminal down. An outbreak of foodborne illness ... (FDA) uses DNA evidence to track down the bacteria that ... It,s not. The FDA has increasingly used a complex, cutting-edge ... illnesses. Put as simply as possible, whole genome sequencing is ...
Breaking Biology Technology: