Navigation Links
Protein structure determined in living cells
Date:3/5/2009

The function of a protein is determined both by its structure and by its interaction partners in the cell. Until now, proteins had to be isolated for analyzing them. An international team of researchers from Tokyo Metropolitan University, Goethe University, and the Frankfurt Institute for Advanced Studies (FIAS) has, for the first time, determined the structure of a protein in its natural environment, the living cell. Using nuclear magnetic resonance (NMR) spectroscopy, the researchers solved the structure of a protein within the bacterium Escherichia coli. "We have reached an important goal of molecular biology", says Prof. Peter Gntert from the Goethe University's Biomolecular Magnetic Resonance Center. (BMRZ) of The research results will be published by the scientific journal Nature on March 5, 2009.

Conventionally, proteins are extracted from the cell, purified, and analyzed in single crystals or in solution. NMR spectroscopy detects signals from the nuclei of hydrogen atoms that are ubiquitous in organic molecules. Measurements in the living cell are challenging because it is difficult to distinguish between the protein of interest and the many other proteins in the cytoplasm. The Japanese researchers around Prof. Yutaka Ito solved this problem by introducing the gene of a putative heavy-metal-binding protein into the model system Escherichia coli, where the protein was in high concentration.

The success of the measurements relies on the method of "in-cell" NMR spectroscopy that was developed a few years ago by Prof. Volker Dtsch from BMRZ at Goethe University. Dtsch was able to attribute signals from living cells to specific proteins that he had labeled with the stable nitrogen isotope N-15. However, it was not possible to calculate a three-dimensional structure. "About two days of measurement time are required to measure a multidimensional NMR spectrum", says Peter Gntert. "Unfortunately, the cells survive for only a 5-6 hours without supply of oxygen and nutrients. Gntert and his colleagues compensated for the concomitant drastic reduction of the measurement time by computational reconstruction of the complete spectrum. Then, they calculated a detailed three-dimensional structure of the protein within E. coli cells using software that was developed in their research group.

The structure determination of proteins by in-cell NMR spectroscopy opens new avenues to investigate at atomic resolution how proteins participate in biological processes in living systems. In-cell NMR spectroscopy advances our understanding of the molecular basis of life, and can contribute to the development of new, better targeted pharmaceuticals.


'/>"/>

Contact: Peter Guentert
guentert@em.uni-frankfurt.de
49-697-982-9621
Goethe University Frankfurt
Source:Eurekalert

Related biology news :

1. Protein function and chromatin structure methods featured in Cold Spring Harbor Protocols
2. Inactivity of proteins behind longer shelf life when freezing
3. Caltech and UCSD researchers shed light on how proteins find their shapes
4. Calculating gene and protein connections in a Parkinsons disease model
5. Inhibiting proteins may prevent cartilage breakdown in arthritis patients
6. Researchers isolate protein domain linked to tumor progression
7. Scientists study full protein content of bakers yeast
8. New research shows high-quality protein in eggs contributes to power, strength and energy
9. New target for medicine to combat Alzheimers: VIB scientists confirm proteins key role
10. Tailor-made recombinant proteins in mammals
11. Long-sought protein structure may help reveal how gene switch works
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/24/2017)...  EyeLock LLC, a leader of iris-based identity ... biometric solution on the latest Qualcomm® Snapdragon™ 835 ... World Congress 2017 (February 27 – March ... 3, Stand 3E10. The Snapdragon ... platform—a combination of hardware, software and biometrics ...
(Date:2/21/2017)... N.Y. and PORTLAND, Ore. ... ) and the Avamere Family of Companies (Avamere Health ... today announced a six-month research study that will apply ... improve eldercare at senior living and health centers. By ... Avamere hopes to gain insights into physical and environmental ...
(Date:2/16/2017)... , Feb. 16, 2017  Genos, a community ... that it has received Laboratory Accreditation from the ... is presented to laboratories that meet stringent requirements ... scientifically rigorous processes. "Genos is committed ... laboratory practices. We,re honored to be receiving CAP ...
Breaking Biology News(10 mins):
(Date:2/24/2017)... ... February 24, 2017 , ... FireflySci, Inc is an ... late 2014, FireflySci had the goal of bringing their powerful cuvette and ... shape the path that FireflySci is going on as they add yet another mark ...
(Date:2/24/2017)... RADNOR, Pa., Feb. 24, 2017  VWR Corporation (NASDAQ: VWR), ... to laboratory and production customers, today reported its financial results ... 2016. Highlights: 4Q16 record ... up 1.0% on an organic basis. ... on an organic basis, while the Americas net sales increased ...
(Date:2/23/2017)... and NEW YORK , Feb. ... Lumeon , a leading digital health company, and ... provider of telemedicine and remote patient monitoring, announce ... telemedicine reimbursements.  DN Telehealth maximizes ... in real-time, extending consultations beyond a physical clinical ...
(Date:2/23/2017)... ... February 23, 2017 , ... BellBrook Labs is formalizing a ... array of biochemical analyses critical for Lead Discovery. The company’s Lead Discovery ... programs, including inhibitor potency and selectivity, mechanism of action, and inhibitor residence times ...
Breaking Biology Technology: