Navigation Links
Protein production: Going viral
Date:1/7/2013

A research team of scientists from EMBL Grenoble and the IGBMC in Strasbourg, France, have, for the first time, described in molecular detail the architecture of the central scaffold of TFIID: the human protein complex essential for transcription from DNA to mRNA. The study, published today in Nature, opens new perspectives in the study of transcription and of the structure and mechanism of other large multi-protein assemblies involved in gene regulation.

By controlling the transcription of DNA into messenger RNA, TFIID forms the cornerstone of the machinery that controls gene expression in our cells. Despite its crucial role, very little was known about its architecture. TFIID is present at very low levels in cells, and it is a very large protein complex made of 20 subunits: this combination largely prevented previous attempts to purify it and decipher its structure and function in molecular detail. Even the most advanced methods for recombinant protein production met their limits when trying to produce its various subunits in the right proportions.

The solution to this bottleneck came from studying the strategy certain viruses, such as Coronaviruses, use when they replicate: they produce very long protein chains that are then divided into individual proteins. Mimicking this technique led to highly abundant and correctly assembled complexes of the core scaffold of TFIID (comprising 10 subunits), which could be purified and analysed at high resolution by combining electron microscopy and data from X-ray crystallography.

This ground-breaking analysis reveals the inner workings of the core-complex of human TFIID in unprecedented detail. It shows that some of its subunits adopt a very defined structure, whereas other parts appear to adopt intricate, extended geometries winding like worms through the complex, holding it together. The overall architecture of the complex is symmetric; however, the authors describe how it becomes asymmetric when it binds to other subunits to finally form the complete TFIID complex.

"We know now in some detail what the core of TFIID looks like, and what happens when further subunits are bound. We believe that we have opened the door to determining the architecture of the entire human TFIID complex in the near future, and likewise of other large multiprotein assemblies involved in gene regulation, and to explain their roles in catalysing biological function," concludes Imre Berger, coordinator of the study at EMBL.


'/>"/>

Contact: Isabelle Kling
isabelle.kling@embl.de
49-622-138-78355
European Molecular Biology Laboratory
Source:Eurekalert

Related biology news :

1. IU biologists offer clearer picture of how protein machine systems tweak gene expression
2. Making memories: How 1 protein does it
3. Embryonic development protein active in cancer growth
4. More effective method of imaging proteins
5. The loss of a protein makes jump the tumor to the lymph node
6. Gold nanoantennas detect proteins
7. The Japanese traditional therapy, honokiol, blocks key protein in inflammatory brain damage
8. New hope for treating Alzheimers Disease: A role for the FKBP52 protein
9. Protein jailbreak helps breast cancer cells live
10. Plant research reveals new role for gene silencing protein
11. Newly found protein helps cells build tissues
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/13/2017)... SANTA MONICA, Calif. , April 13, 2017 /PRNewswire/ ... New York will feature emerging and ... Innovation Summits. Both Innovation Summits will run alongside the ... variety of speaker sessions, panels and demonstrations focused on ... east coast,s largest advanced design and manufacturing event will ...
(Date:4/11/2017)... Research and Markets has announced the addition of the ... ... grow at a CAGR of 30.37% during the period 2017-2021. ... prepared based on an in-depth market analysis with inputs from industry ... over the coming years. The report also includes a discussion of ...
(Date:4/6/2017)... Forecasts by Product Type (EAC), Biometrics, Card-Based ... & Logistics, Government & Public Sector, Utilities / Energy ... Nuclear Power), Industrial, Retail, Business Organisation (BFSI), Hospitality & ... for a definitive report on the $27.9bn Access Control ... ...
Breaking Biology News(10 mins):
(Date:4/27/2017)... ... April 27, 2017 , ... ... Borlaug CAST Communication Award goes to Jayson Lusk, a consummate communicator who promotes ... media to advocate for science, as he explains how innovation and growth in ...
(Date:4/27/2017)...  Kinexum, a distinguished resource for research, development and ... of Thomas C. Seoh as President and ... founder, who becomes Executive Chairman and will continue to ... Thomas Seoh commented, "I am excited ... lead the firm,s remarkable team of life science professionals, ...
(Date:4/26/2017)... ... 26, 2017 , ... WonderWorks, Myrtle Beach’s science focused amusement ... of deep space exploration and inspire space enthusiasts. The exhibit features interactive exhibits ... guest appearance by former Shuttle Astronaut Don Thomas. , The intergalactic weekend kicks ...
(Date:4/26/2017)... ... ... LABS, Inc. (LABS) announced in December 2016 that two new Zika ... Testing (NAT) for ZIKV; and Enzyme Immunoassays (EIAs) specific for IgM and IgG ZIKV ... donors under an Investigational New Drug (IND) study protocol. , Now, as part ...
Breaking Biology Technology: