Navigation Links
Protein production: Going viral
Date:1/7/2013

A research team of scientists from EMBL Grenoble and the IGBMC in Strasbourg, France, have, for the first time, described in molecular detail the architecture of the central scaffold of TFIID: the human protein complex essential for transcription from DNA to mRNA. The study, published today in Nature, opens new perspectives in the study of transcription and of the structure and mechanism of other large multi-protein assemblies involved in gene regulation.

By controlling the transcription of DNA into messenger RNA, TFIID forms the cornerstone of the machinery that controls gene expression in our cells. Despite its crucial role, very little was known about its architecture. TFIID is present at very low levels in cells, and it is a very large protein complex made of 20 subunits: this combination largely prevented previous attempts to purify it and decipher its structure and function in molecular detail. Even the most advanced methods for recombinant protein production met their limits when trying to produce its various subunits in the right proportions.

The solution to this bottleneck came from studying the strategy certain viruses, such as Coronaviruses, use when they replicate: they produce very long protein chains that are then divided into individual proteins. Mimicking this technique led to highly abundant and correctly assembled complexes of the core scaffold of TFIID (comprising 10 subunits), which could be purified and analysed at high resolution by combining electron microscopy and data from X-ray crystallography.

This ground-breaking analysis reveals the inner workings of the core-complex of human TFIID in unprecedented detail. It shows that some of its subunits adopt a very defined structure, whereas other parts appear to adopt intricate, extended geometries winding like worms through the complex, holding it together. The overall architecture of the complex is symmetric; however, the authors describe how it becomes asymmetric when it binds to other subunits to finally form the complete TFIID complex.

"We know now in some detail what the core of TFIID looks like, and what happens when further subunits are bound. We believe that we have opened the door to determining the architecture of the entire human TFIID complex in the near future, and likewise of other large multiprotein assemblies involved in gene regulation, and to explain their roles in catalysing biological function," concludes Imre Berger, coordinator of the study at EMBL.


'/>"/>

Contact: Isabelle Kling
isabelle.kling@embl.de
49-622-138-78355
European Molecular Biology Laboratory
Source:Eurekalert

Related biology news :

1. IU biologists offer clearer picture of how protein machine systems tweak gene expression
2. Making memories: How 1 protein does it
3. Embryonic development protein active in cancer growth
4. More effective method of imaging proteins
5. The loss of a protein makes jump the tumor to the lymph node
6. Gold nanoantennas detect proteins
7. The Japanese traditional therapy, honokiol, blocks key protein in inflammatory brain damage
8. New hope for treating Alzheimers Disease: A role for the FKBP52 protein
9. Protein jailbreak helps breast cancer cells live
10. Plant research reveals new role for gene silencing protein
11. Newly found protein helps cells build tissues
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/23/2017)... the first robotic gym for the rehabilitation and functional motor sense evaluation ... Genoa, Italy . The first 30 robots will be available from ... . The technology was developed and patented at the IIT laboratories ... Technology thanks to a 10 million euro investment from entrepreneur Sergio Dompè. ... ...
(Date:5/16/2017)... 16, 2017   Bridge Patient Portal , ... MD EMR Systems , an electronic medical ... GE, have established a partnership to build an ... the GE Centricity™ products, including Centricity Practice Solution ... These new integrations will allow healthcare delivery ...
(Date:4/24/2017)... 24, 2017 Janice Kephart , ... Identity Strategy Partners, LLP (IdSP) , today issues ... President Trump,s March 6, 2017 Executive Order: ... vetting can be instilled with greater confidence, enabling ... all refugee applications are suspended by until at ...
Breaking Biology News(10 mins):
(Date:8/22/2017)... ... August 22, 2017 , ... Cutting Edge ... of highly differentiated spinal implant technologies, announced today the appointment of Luke Maher ... spinal device industry experience to directing Cutting Edge Spine’s national sales force, including ...
(Date:8/22/2017)... ... 2017 , ... KBioBox is pleased to announce the creation ... ananlysis. KBioBox has adapted their core technology and direct alignment algorithms to extend ... understand reports, extended indel analysis, and translocation analysis. , “The extended GUIDE-Seq analysis ...
(Date:8/21/2017)... ... 2017 , ... MacArthur Sotheby’s International Realty, a luxury real ... the first Delos Wellness Signature™ residence in Hawaii is on the market for ... listing agent Kelly Allen, R(S) of Carvill Sotheby’s International Realty located on Oahu, ...
(Date:8/21/2017)... ... August 21, 2017 , ... Baltimore ... bio security firm, to bring its proprietary CANARY pathogen detection technology and high ... the Chinese firm has purchased an undisclosed number of PathSensors’ Zephyr pathogen detection ...
Breaking Biology Technology: