Navigation Links
Protein improves efficacy of tumor-killing enzyme
Date:4/29/2013

Scientists have devised a method for delivering tumor cell-killing enzymes in a way that protects the enzyme until it can do its work inside the cell. In their study in mBio, the online open-access journal of the American Society for Microbiology, researchers assembled microscopic protein packages that can deliver an enzyme called PEIII to the insides of cells. By attaching a protein called ubiquitin to the enzyme, they were able to protect it from degradation by the cell, allowing the enzyme to complete its mission. The results indicate that ubiquitin may be a useful addition to targeted toxins.

Although researchers have been developing tumor-directed "targeted toxins" for decades, their success has been hindered by technical problems, including inadequate tumor specificity, low efficiency of delivery to the interior of the cell (also called the cytosol), and other issues. In this study, researchers from the National Institute of Allergy and Infectious Diseases sought to improve the persistence of the enzyme in the cytosol.

They created bundles of proteins designed to carry this out. The targeted toxin assembly included two components the researchers have used before in targeted toxins: the "killing" enzyme PEIII, and a set of targeting proteins called LFn that deliver the PEIII enzyme via pores to the inside of the cell. The LFn delivery system was engineered to specifically target and attach to tumor cells.

The third component in the bundle was a new addition: ubiquitin, a small protein that is normally used by cells to target waste proteins for degradation. The researchers inserted ubiquitin in between the LFn and the PEIII, then tested the bundle on mice with tumors. The idea was to use the cell's own ubiquitin-cleaving enzymes to cut the ubiquitin off and free up the PEIII enzyme once it's inside the cell.

The system worked. Tumor growth was inhibited in mice treated with targeted toxins that either carried t
'/>"/>

Contact: Jim Sliwa
jsliwa@asmusa.org
202-942-9297
American Society for Microbiology
Source:Eurekalert

Page: 1 2

Related biology news :

1. Rare, lethal childhood disease tracked to protein
2. National survey highlights perceived importance of dietary protein to prevent weight gain
3. Scientists discover new way protein degradation is regulated
4. Science surprise: Toxic protein made in unusual way may explain brain disorder
5. Metabolic fingerprinting: Using proteomics to identify proteins in gymnosperm pollination drops
6. AACR news: Six2 homeoprotein allows breast cancer cells to detach and metastasize
7. A proteins well-known cousin sheds light on its gout-linked relative
8. Mechanism of mutant histone protein in childhood brain cancer revealed
9. Pitt team finds immunity protein that ramps up inflammation, and agents that can block it
10. Market Analysis: Biomaterials and Engineered Protein
11. Transmission routes of spreading protein particles
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/25/2015)... According to a new ... Area), Technology, Material (Optical Prism, Piezoelectric, Capacitive & ... Others) & Geography - Global Forecast to 2014 ... is expected to reach $14,500.07 Million by 2020, ... Browse 76 market Tables and 109 Figures spread ...
(Date:6/25/2015)... June 25, 2015  TAKE Solutions Ltd., a global ... the United States Patent and Trademark Office (USPTO) for ... leverages TAKE Solutions, Clinical Accelerators to reduce the time ... compared to standardization without the accelerators), thus reducing time ... At the heart of the patented Clinical ...
(Date:6/24/2015)... June 24, 2015 This report provides market ... next six years. It contains an analysis of the ... with their impact from the short, medium, and long ... the industry, market, and technology trends that currently, prevail ... of concerned authorities to efficiently manage the increasing marine ...
Breaking Biology News(10 mins):Fingerprint Sensors Market Worth $14,500.07 Million by 2020 2Fingerprint Sensors Market Worth $14,500.07 Million by 2020 3Fingerprint Sensors Market Worth $14,500.07 Million by 2020 4TAKE Solutions Awarded Patent By USPTO 2Automatic Identification System Market by Class, by Platform, by Application and by Geography - Global Forecasts & Analysis to 2014 - 2020 2
... Eighty percent of American voters favor national standards that ... la carte foods sold in U.S. schools and encourage the ... a new poll commissioned by the Kids, Safe & Healthful ... and the Robert Wood Johnson Foundation. The U.S. ...
... this month on ecological changes at 26 global research sites ... in the high mountains west of the city -- indicates ... most sensitive to changes in climate. The six ... The papers were tied to data gathered at sites ...
... Academy of Sciences will host the Fifth Annual ... design of oligosaccharides and proteins for materials and ... on the following areas: (1) synthetic and biosynthetic ... these macromolecules in materials science and glycobiology, (2) ...
Cached Biology News:Poll shows strong voter support for school nutrition standards 2Poll shows strong voter support for school nutrition standards 3CU research shows warming climate threatens ecology at mountain research site west of Boulder 2CU research shows warming climate threatens ecology at mountain research site west of Boulder 3
(Date:7/28/2015)... , July 28, 2015 Seahorse ... cell metabolism, is the provider of XF Technology ... all disease research areas. As the links between ... into cell metabolism is escalating rapidly.   ... by the Nature Publishing Group (NPG) featuring XF ...
(Date:7/28/2015)... -- Research and Markets ( ... "Analysis of the Global Flow Cytometry Market ... Globally, flow cytometry remains a popular tool for ... stem cells. But the market is witnessing active ... from underdeveloped nations to quantitate CD4 cell markers ...
(Date:7/28/2015)... ... July 28, 2015 , ... VetStem Biopharma will be offering ... credentialing course to local area veterinarians. , The tour and course will be given ... no charge) at VetStem’s Poway facility. Staff members are welcome to attend with their ...
(Date:7/28/2015)... , July 28, 2015 Tauriga Sciences, ... diversified life sciences company, today announced that its stockholders ... of common stock of the Company from 1,000,000,000 to ... July 27, 2015 at the Law Offices of Nixon ... the Special Meeting, there were 480,655,929 shares of common ...
Breaking Biology Technology:XF Technology Used to Demonstrate Feasibility of Stem Cell Regenerative Technique 2XF Technology Used to Demonstrate Feasibility of Stem Cell Regenerative Technique 3Analysis of the Global Flow Cytometry Market 2012-2018 with a 2014 Base Year and a 2015-2018 Forecast Period 2VetStem Biopharma weekly veterinary continuing education classes and GMP manufacturing facility tour 2Tauriga Sciences, Inc. Stockholders Approve Increase In Authorized Common Stock 2Tauriga Sciences, Inc. Stockholders Approve Increase In Authorized Common Stock 3
... Opportunities in Transplant Diagnostics, Genetic Testing and Pharmaceutical ... 8 Gen-Probe (Nasdaq: GPRO ) ... acquisition of Tepnel Life Sciences, plc, a rapidly ... in the United Kingdom, for 27.1 pence ($0.40)(1) ...
... (TSX-V: ENW) ("EnWave" or "the Company") today announced ... developed powderREV technology in the dehydration of cultures ... tests were conducted as the first phase of ... objective of the agreement is to create a ...
... 7 /PRNewswire-Asia-FirstCall/ -- Biostar Pharmaceuticals,Inc. ("Biostar" or the "Company") (OTC Bulletin Board: ... , , Highlights for Year Ended December 31, 2008:, ... million, up from $15.9 million, for ... to $6,690,542 as compared to, $3,963,524 ...
Cached Biology Technology:Gen-Probe Completes Acquisition of Tepnel Life Sciences 2Gen-Probe Completes Acquisition of Tepnel Life Sciences 3Gen-Probe Completes Acquisition of Tepnel Life Sciences 4EnWave Announces Collaboration and Initial Testing Results with Danisco AS 2EnWave Announces Collaboration and Initial Testing Results with Danisco AS 3Biostar Pharmaceuticals, Inc. Reports Record Revenues and Earnings for 2008 2Biostar Pharmaceuticals, Inc. Reports Record Revenues and Earnings for 2008 3Biostar Pharmaceuticals, Inc. Reports Record Revenues and Earnings for 2008 4Biostar Pharmaceuticals, Inc. Reports Record Revenues and Earnings for 2008 5Biostar Pharmaceuticals, Inc. Reports Record Revenues and Earnings for 2008 6
Conjugation services for Antibodies, Proteins, Oligos, Peptides, Beads, bioconjugation, antibody-oligonucleotide, LNA peptide, LNA protein, LNA antibody, HRP, Alkaline phosphatase, phycoerythrin, FIT...
Blimp-1 (H-150)...
SCP-1 (P-17)...
Mouse Galectin-1 Affinity Purified Polyclonal Ab...
Biology Products: