Navigation Links
Protein function and chromatin structure methods featured in Cold Spring Harbor Protocols
Date:3/3/2009

COLD SPRING HARBOR, N.Y. (Tues., March 3, 2009) Two new methods for analyzing the roles played by proteins in cells are featured in the March issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc3_09.dtl). Thomas J. Wandless and colleagues from Stanford University (http://wandless.stanford.edu/) provide detailed instructions for "Regulating Protein Stability in Mammalian Cells Using Small Molecules." This technique provides a rapid, reversible, and tunable method for studying the activity of a protein of interest in cells. The protein is attached to a destabilization domain, and the resulting fusion proteins are unstable and degraded, except in the presence of small ligands. The speed of small-molecule binding makes it an attractive alternative to studying gene function using RNA interference (RNAi). The method is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2009/3/pdb.prot5172).

The second featured article for March looks at long-range chromosomal interactions and the proteins that mediate them. Stephen Baylin and colleagues from Johns Hopkins University (http://humangenetics.jhmi.edu/index.php?option=com_content&task=view&id=21&Itemid=35) contribute a method for the "Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein Mediated Genome Architecture." 6C technology combines multiple techniques to identify the proteins that bridge distant genomic regions, while simultaneously identifying physical proximities. The method is also useful for determining if a candidate protein might mediate long-range interactions, both in cis and in trans in the nucleus. The 6C assay advances our understanding of chromatin folding and provides an important tool to examine the role of specific proteins in nuclear organization. This method is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2009/3/pdb.prot5168).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. Inactivity of proteins behind longer shelf life when freezing
2. Caltech and UCSD researchers shed light on how proteins find their shapes
3. Calculating gene and protein connections in a Parkinsons disease model
4. Inhibiting proteins may prevent cartilage breakdown in arthritis patients
5. Researchers isolate protein domain linked to tumor progression
6. Scientists study full protein content of bakers yeast
7. New research shows high-quality protein in eggs contributes to power, strength and energy
8. New target for medicine to combat Alzheimers: VIB scientists confirm proteins key role
9. Tailor-made recombinant proteins in mammals
10. Long-sought protein structure may help reveal how gene switch works
11. Scientists uncover new class of non-protein coding genes in mammals with key functions
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/9/2016)... -- Paris Police Prefecture ... to ensure the safety of people and operations in several ... tournament Teleste, an international technology group specialised in ... that its video security solution will be utilised by ... safety across the country. The system roll-out is scheduled for ...
(Date:6/2/2016)... Perimeter Surveillance & Detection Systems, Biometrics & ... & Other Service  The latest report from ... of the global Border Security market . Visiongain ... billion in 2016. Now: In November 2015 ... and hardware technologies for advanced video surveillance. ...
(Date:5/12/2016)... , May 12, 2016 WearablesResearch.com ... just published the overview results from the Q1 wave ... the recent wave was consumers, receptivity to a program ... data with a health insurance company. "We ... to share," says Michael LaColla , CEO of ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... 27, 2016  Global demand for enzymes is ... 2020 to $7.2 billion.  This market includes enzymes ... products, biofuel production, animal feed, and other markets) ... biocatalysts). Food and beverages will remain the largest ... consumption of products containing enzymes in developing regions.  ...
(Date:6/27/2016)... ... June 27, 2016 , ... Parallel 6 , the leading ... the Clinical Reach Virtual Patient Encounter CONSULT module which enables both audio ... and clinical trial team. , Using the CONSULT module, patients and physicians can schedule ...
(Date:6/27/2016)... , June 27, 2016   Ginkgo Bioworks ... to industrial engineering, was today awarded as one ... selection of the world,s most innovative companies. Ginkgo ... scale for the real world in the nutrition, ... engineers work directly with customers including Fortune 500 ...
(Date:6/24/2016)... ... 2016 , ... Researchers at the Universita Politecnica delle Marche in Ancona combed ... pleural mesothelioma. Their findings are the subject of a new article on the Surviving ... signposts in the blood, lung fluid or tissue of mesothelioma patients that can help ...
Breaking Biology Technology: