Navigation Links
Protein function and chromatin structure methods featured in Cold Spring Harbor Protocols
Date:3/3/2009

COLD SPRING HARBOR, N.Y. (Tues., March 3, 2009) Two new methods for analyzing the roles played by proteins in cells are featured in the March issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc3_09.dtl). Thomas J. Wandless and colleagues from Stanford University (http://wandless.stanford.edu/) provide detailed instructions for "Regulating Protein Stability in Mammalian Cells Using Small Molecules." This technique provides a rapid, reversible, and tunable method for studying the activity of a protein of interest in cells. The protein is attached to a destabilization domain, and the resulting fusion proteins are unstable and degraded, except in the presence of small ligands. The speed of small-molecule binding makes it an attractive alternative to studying gene function using RNA interference (RNAi). The method is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2009/3/pdb.prot5172).

The second featured article for March looks at long-range chromosomal interactions and the proteins that mediate them. Stephen Baylin and colleagues from Johns Hopkins University (http://humangenetics.jhmi.edu/index.php?option=com_content&task=view&id=21&Itemid=35) contribute a method for the "Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein Mediated Genome Architecture." 6C technology combines multiple techniques to identify the proteins that bridge distant genomic regions, while simultaneously identifying physical proximities. The method is also useful for determining if a candidate protein might mediate long-range interactions, both in cis and in trans in the nucleus. The 6C assay advances our understanding of chromatin folding and provides an important tool to examine the role of specific proteins in nuclear organization. This method is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2009/3/pdb.prot5168).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. Inactivity of proteins behind longer shelf life when freezing
2. Caltech and UCSD researchers shed light on how proteins find their shapes
3. Calculating gene and protein connections in a Parkinsons disease model
4. Inhibiting proteins may prevent cartilage breakdown in arthritis patients
5. Researchers isolate protein domain linked to tumor progression
6. Scientists study full protein content of bakers yeast
7. New research shows high-quality protein in eggs contributes to power, strength and energy
8. New target for medicine to combat Alzheimers: VIB scientists confirm proteins key role
9. Tailor-made recombinant proteins in mammals
10. Long-sought protein structure may help reveal how gene switch works
11. Scientists uncover new class of non-protein coding genes in mammals with key functions
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/8/2017)... Feb. 7, 2017 Report Highlights ... 2021 from $8.3 billion in 2016 at a compound ... 2021. Report Includes - An overview of the ... trends, with data from 2015 and 2016, and projections ... Segmentation of the market on the basis of product ...
(Date:2/6/2017)... , Feb. 6, 2017 According ... security are driving border authorities to continue to ... reports there are 2143 Automated Border Control (ABC) ... currently deployed at more than 163 ports of ... 2013 to 2016 achieving a combined CAGR of ...
(Date:2/2/2017)... , Feb. 2, 2017  EyeLock LLC, a market ... new white paper " What You Should Know About ... of ensuring user authenticity is a growing concern. In ... of users. However, traditional authentication schemes such as username/password ... Biometric authentication offers an elegant solution to the ...
Breaking Biology News(10 mins):
(Date:2/24/2017)... , Feb. 24, 2017  OncoSec Medical Incorporated ("OncoSec") ... will host a Key Opinion Leader event to highlight ... oral and poster presentation at the upcoming 2017 ASCO-SITC ... KOL event will be held in-person and via live ... / 9:00 AM PST at the Lotte New York ...
(Date:2/23/2017)... Calif. , Feb. 23, 2017  MIODx ... license for two key immunotherapy technologies from the ... technology provides a method to monitor a patient ... as PD-L1 and CTLA-4.  The second license extends ... a patient is likely to have an immune-related ...
(Date:2/23/2017)... (PRWEB) , ... February 23, ... ... Inc., announced today that in a published evaluation of multiple immunoassay-based threat ... U.S. Department of Energy Laboratory, PathSensors’ CANARY® biosensor threat detection technology was ...
(Date:2/23/2017)... 23, 2017 Aviva Systems Biology Corporation ... acquisition of GenWay Biotech Incorporated, a protein solutions ... product offering for both the research and diagnostic ... and enhance capabilities for both entities. GenWay,s 18 years ... will nicely complement ASB,s objective to become a ...
Breaking Biology Technology: