Navigation Links
Protein 'filmed' while unfolding at atomic resolution
Date:2/11/2013

By combining low temperatures and NMR spectroscopy, the scientists visualized seven intermediate forms of the CylR2 protein while cooling it down from 25C to -16C. Their results show that the most instable intermediate form plays a key role in protein folding. The scientists' findings may contribute to a better understanding of how proteins adopt their structure and misfold during illness.

Whether Alzheimer's, Parkinson's or Huntington's Chorea all three diseases have one thing in common. They are caused by misfolded proteins that form insoluble clumps in the brains of affected patients and, finally, destroy their nerve cells. One of the most important questions in the biological sciences and medicine is thus: How do proteins the tools of living cells achieve or lose their three-dimensional structure. Because only if their amino acid chains are correctly folded, can proteins perform their tasks properly.

But what exactly happens when proteins fold or unfold was previously nearly impossible to investigate. With heat and pressure, proteins easily lose their shape and thus their function. However, such methods are not suitable for directly observing their unfolding process. The intermediate forms that occur in the course of protein folding are much too transient.

With a novel approach, researchers have now succeeded in "filming" the complex process of protein folding for the first time. Scientists at the Max Planck Institute for Biophysical Chemistry (MPIbpc) and the German Center for Neurodegenerative Diseases (DZNE) in Gttingen, together with their colleagues at the Polish Academy of Sciences in Warsaw and at the University of Warsaw, have rendered visible at atomic resolution how a protein progressively "loses its shape". In doing so, the researchers had pinned their hopes on low temperatures. "If a protein is slowly cooled down, its intermediate forms accumulate in larger quantities than in commonly used denaturation methods,
'/>"/>

Contact: Dr. Dirk Frger
presse@dzne.de
0049-228-433-02260
Helmholtz Association of German Research Centres
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Key protein revealed as trigger for stem cell development
2. Compound stimulates tumor-fighting protein in cancer therapy
3. FASEB SRC announces conference registration open for: Arf and Rab Family G Proteins
4. FASEB SRC announces: Molecular Mechanisms & Physiological Consequences of Protein Aggregation
5. FASEB SRC announces conference: Matricellular Proteins in Development, Health, and Disease
6. Protein origami: Quick folders are the best
7. Pitt team finds Achilles Heel of key HIV replication protein
8. First special edition updating progress on efforts to map human proteins
9. Study: Odd biochemistry yields lethal bacterial protein
10. Protein structure: Immune system foiled by a hairpin
11. How the protein transport machinery in the chloroplasts of higher plants developed
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/24/2014)... Carbon offsetting initiatives could be improved with new insights ... , Scientists studying the Amazon Basin have revealed ... trees across the region by comparing satellite maps with ... enable researchers to assess more accurately the amount of ... factor in carbon offset schemes, in which trees are ...
(Date:4/24/2014)... turtle in North America, weighing in at up to ... from Florida and the University of Vermont have discovered ... , Examining museum specimens and wild turtles, the scientists ... Once heavily hunted for turtle meat alligator snapper ... the 1960s the riverine populations have been deeply ...
(Date:4/24/2014)... out a welcome mat to bacteria seeking to invade, and ... published this week in the Proceedings of the National ... between microbe and host that researchers can exploit to protect ... plant Arabidopsis puts out a molecular signal that ... hostile army were unknowingly passing by a castle, and the ...
Breaking Biology News(10 mins):Amazon rainforest survey could improve carbon offset schemes 2Two new US turtle species described 2Two new US turtle species described 3How a plant beckons the bacteria that will do it harm 2How a plant beckons the bacteria that will do it harm 3
... Current arthritis medications can ease the pain, but ... aggressive treatments: use of very limited available drugs ... to find new therapeutic targets for arthritis by ... interrupted, may prevent arthritis pain caused by joint ...
... More than 300 people and 30 companies are expected ... career fair on March 13, 2009 at NJIT. The ... 3:30 p.m. in NJIT,s Campus Center, unites industry professionals ... "Explore the Leading Edge of the ...
... by the Wildlife Conservation Society, have helped the government ... protect more than 600 gorillas, along with other threatened ... Called Deng Deng National Park, the new ... kilometers) in sizeapproximately the size of the City of ...
Cached Biology News:Inhibiting proteins may prevent cartilage breakdown in arthritis patients 2NJIT to host major biomedical engineering showcase and career fair 2Wildlife Conservation Society helps Cameroon create new national park 2Wildlife Conservation Society helps Cameroon create new national park 3
(Date:1/15/2014)... 2014 Cynvenio, a cancer diagnostics company ... genomic analysis of tumor cells in the bloodstream, today ... cancer decision support application powered by CollabRx, Inc. ... genomic test, Therapy Finder will provide breast cancer patients ...
(Date:1/15/2014)... January 15, 2014 This webinar will ... and clinical safety assessment in biosimilars. , Regulatory frameworks ... biosimilar drug development, however the complex nature of biopharmaceuticals ... safety and efficacy extremely challenging. Based on the specific ...
(Date:1/15/2014)... 2014 Two champions of science, technology, ... of an annual competition for middle and high school ... innovative STEM study. The competition presents students with real-world ... Engineering Aptitude, Mathematics, and Sciences is a program ...
(Date:1/15/2014)... Arizona (PRWEB) January 15, 2014 2013 ... for Scottsdale’s Brain State Technologies®. They saw continued independent ... Medical Center who were awarded a $1 million grant ... published in “Brain and Behavior” a peer reviewed journal, ...
Breaking Biology Technology:Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 3Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 2Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 3Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 4Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 5Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3
... March 25 Alseres,Pharmaceuticals, Inc. (Nasdaq: ALSE ) ... on Tuesday, April 1st, 2008 at 10:00am EDT to ... the Company., To access the conference call, please ... this conference call is,7767549. Please dial in 5 to ...
... PALO ALTO, Calif., March 25 Varian Medical,Systems (NYSE: VAR ) ... 2008 following the close of regular trading on,Wednesday, April 23, 2008. The ... at 2:00 p.m. PT., , The Varian Medical Systems quarterly results ... Contact Anne Rambo via phone at 650-424-5834 or ...
... Codexis, Inc., today,announced the appointment of David Anton, ... Research and Development. He,joins Codexis from DuPont, where ... Alan Shaw, Ph.D., President and CEO., "Codexis ... technology can make a significant impact," said Alan ...
Cached Biology Technology:Alseres Pharmaceuticals to Host 2007 Financial Results Conference Call 2Alseres Pharmaceuticals to Host 2007 Financial Results Conference Call 3Varian Medical Systems Schedules Second Quarter FY2008 News Release and Conference Call 2Varian Medical Systems Schedules Second Quarter FY2008 News Release and Conference Call 3Codexis Names David Anton VP Bioindustrials R&D 2
Recombinant Rhesus Monkey IFN-Alpha...
2,4-Dinitrophenyl hapten is conjugated to BSA (Bovine Serum Albumin) protein through lysine....
4-Hydroxy-3-iodo-5-nitrophenylacetyl hapten is conjugated to BSA protein through lysine by amide bonds....
Amino Acid Analysis is performed using ion-exchange separation of the amino acids from acid hydrolysates, followed by ninhydrin detection. Typical quantities required are 0.1 to 0.5 mg, depending on...
Biology Products: