Navigation Links
Protein extremes gain relevance in massive proteomic studies
Date:6/15/2010

Researchers at University of Ghent, Belgium, and Universitat Autnoma de Barcelona (UAB) develop a new procedure to identify the two extremes of protein molecules and their processing and maturing in in-vivo and ex-vivo massive proteomic studies. The research has been published online at Nature Methods, under the title of "Complementary Positional Proteomics for Screening of Endo- and Exoproteases".

The world of proteins is one of the most complex and fundamental in living beings given that these biomolecules carry out and control or intervene in most biological functions. The massive identification and characterisation in a multitude of live organisms and in different vital stages has been and remains one of the most important tasks of proteomics, since it allows for further calibration of their role in biological functions and pathologies/diseases and to develop control strategies (e.g. drugs and vaccines).

Until recently most proteomic studies were focused on internal regions of proteins, usually those located in the three-dimensional folding (essential for many functions) or in the N-terminal (-Nt), with a free amine group (-NH2) at the start of the linear chain of linked amino acids. Protein molecules can have either few or hundreds (common in many organisms) and even thousands of linked amino acids, always in linear form, even if three-dimensional folding later takes place. This focalised research is due to the fact that procedures and chemistry needed to study N-terminal and internal regions are easier and can be developed quicker.

The new method developed by scientists helps to expand studies to C-terminal proteins (-Ct), proteins with a free carboxyl group, COOH, at the end of the linear chain of amino acids. The global and integrated strategy was created by research groups of the University of Ghent, Belgium (Petra van Damme, Kris Gevaert, et. al.) and UAB (Slvia Bronsoms and Francesc Xavier Aviles from the Institute of Biotechnology and Biomedicine and the Department of Biochemistry and Molecular Biology).

In other words, it is now possible to use massive proteomic methods to study maturing processes of proteins which often include breaks in their linear sequence both internally and at their N- and C-termini. Therefore, it will be possible to assess the specific role of these regions in the processes mentioned and in other related functions such as generation, activation and deactivation of hormones, growth factors, neuropeptides, enzymes, membrane receptors and many other proteins with important biological roles. The strategy developed can be carried out ex-vivo (in cell extracts) or in-vivo (by transfection and proteomic analysis). In addition, protein extremes are commonly involved in essential functions, such as locating and directing cells-tissues, triggering the start of three-dimensional folding, joining other biomolecules and macrostructures, post-generation chemical modifications of proteins, etc.

This publication completes previous studies (e.g. on N-terminal regions) carried out by the Flemish research group, leader in the field of general proteomics, as well as by researchers at Universitat Autnoma de Barcelona, leaders in the field of enzymes processing and maturing C-terminal proteins (carboxypeptidases), who recently published an article on the processing of C-terminal peptides in the Journal of Biological Chemistry, by S. Tanco, J. Lorenzo and F. X. Aviles, of UAB, and Ll. Fricker et. al. of Albert Einsten College of Medicine of New York.


'/>"/>

Contact: Maria Jesus Delgado
MariaJesus.Delgado@uab.cat
34-935-814-049
Universitat Autonoma de Barcelona
Source:Eurekalert  

Related biology news :

1. Protein could heal erectile dysfunction after cancer surgery
2. A balanced protein diet can reduce accumulation of nitrogen on dairy cattle farms by up to 35 percent
3. New function discovered in cancer prevention protein
4. Neural tissue contains imbalanced levels of proteins, U-M study finds
5. MIT chemists design new way to fluorescently label proteins
6. Bodys own proteins may lead the way in global fight against tuberculosis
7. Novel protein essential for successful pregnancy
8. Protein regulates enzyme linked to Alzheimers disease
9. Intracellular express -- why transport protein molecules have brakes
10. Protein power for Jack and the beanstalk
11. Study finds protein that plays key role in early embryonic development
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Protein extremes gain relevance in massive proteomic studies
(Date:4/24/2017)... , April 24, 2017 ... and partner with  Identity Strategy Partners, LLP (IdSP) ... "With or without President Trump,s March 6, 2017 ... Terrorist Entry , refugee vetting can be instilled with ... resettlement. (Right now, all refugee applications are suspended ...
(Date:4/13/2017)... SANTA MONICA, Calif. , April 13, 2017 /PRNewswire/ ... New York will feature emerging and ... Innovation Summits. Both Innovation Summits will run alongside the ... variety of speaker sessions, panels and demonstrations focused on ... east coast,s largest advanced design and manufacturing event will ...
(Date:4/11/2017)... BROOKLYN, N.Y. , April 11, 2017 /PRNewswire-USNewswire/ ... identical fingerprints, but researchers at the New York ... University College of Engineering have found that partial ... fingerprint-based security systems used in mobile phones and ... previously thought. The vulnerability lies in ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... DuPont Pioneer and ... they have entered into a multiyear collaboration to identify and characterize novel CRISPR-Cas ... tools for gene editing across all applications. , Under the terms of the ...
(Date:10/12/2017)... ... October 12, 2017 , ... ... analysis platform specifically designed for life science researchers to analyze and interpret ... Rosalind Franklin, who made a major contribution to the discovery of the ...
(Date:10/11/2017)... ... 2017 , ... ComplianceOnline’s Medical Device Summit is back for its 4th year. ... San Francisco, CA. The Summit brings together current and former FDA office bearers, regulators, ... government officials from around the world to address key issues in device compliance, quality ...
(Date:10/11/2017)... ... ... Disappearing forests and increased emissions are the main causes of the evolving air ... living in larger cities are affected by air pollution related diseases. , That is ... globally - decided to take action. , “I knew I had to take action ...
Breaking Biology Technology: