Navigation Links
Protein-bait interactions, display libraries featured in Cold Spring Harbor Protocols

COLD SPRING HARBOR, N.Y. (Mon., Mar. 1, 2010) -- The use of recombinant proteins, antibodies, small molecules, or nucleic acids as affinity reagents is a simple yet powerful strategy to study the protein/bait interactions that drive biological processes. Analysis via mass spectrometry rather than western blotting extends the identification of interactors, often allowing detection of thousands of proteins from complex mixtures. But this increased sensitivity can lead to problems distinguishing specific interactions from background noise. In the March issue of Cold Spring Harbor Protocols (, Shao-En Ong from the Broad Institute of MIT and Harvard ( presents "Unbiased Identification of Protein-Bait Interactions Using Biochemical Enrichment and Quantitative Proteomics." This method uses quantitative proteomics approaches to compare enrichment with the bait of interest against samples using control baits to allow sensitive detection and discrimination of specific protein/bait interactions. As one of March's featured articles, it is freely available on the journal's website (

The baculovirus expression vector system has been widely used to produce proteins originating from both prokaryotic and eukaryotic sources. It offers easy cloning techniques and abundant viral propagation, and since it is based on an insect cell environment, it provides eukaryotic posttranslational modification machinery. Surface modifications of the viral capsid enable specific targeting. Such modifications can be used to enhance viral binding and entry to a wide variety of both dividing and nondividing mammalian cells, as well as to produce antibodies against the displayed antigen. In addition, the technology should enable modifications of intracellular behavior, i.e., trafficking of recombinant "nanoparticles," a highly relevant feature for studies of targeted gene or protein delivery. In the March issue of Cold Spring Harbor Protocols (, Christian Oker-Blom and colleagues ( provide a suite of articles detailing the use of baculovirus-based display and gene delivery systems. Their protocol for "Creation of Baculovirus Display Libraries" is featured, and is freely available on the journal's website (


Contact: David Crotty
Cold Spring Harbor Laboratory

Related biology news :

1. GestureTek Unveils The CUBE V3 Portable, Plug and Play Interactive Projection Display System for Advertising and Exhibits
2. GestureTek Unveils The CUBE V3 Portable, Plug and Play Interactive Projection Display System for Advertising and Exhibits
3. Loyal alligators display the mating habits of birds
4. U.S. Navy Awards Lockheed Martin Six-year Contract Worth Up to $72 Million for Advanced Display Systems Support
5. Plants display molecular amnesia
6. Scripps Research scientists create new way to screen libraries of 10 million or more compounds
7. Using combinatorial libraries to engineer genetic circuits advances synthetic biology
8. Advances in cancer detection research by Virginia Tech engineer featured in British magazine
9. High-throughput genotyping, protein purification featured in Cold Spring Harbor Protocols
10. Vertical farming advocate to be featured at upcoming free lecture at NJIT
11. Methods for gene transfer in stem cells featured in Cold Spring Harbor Protocols
Post Your Comments:
(Date:10/8/2015)... SAN JOSE, Calif. , Oct. 8, 2015 ... leading developer of human interface solutions, announced today ... first quarter of fiscal 2016 on Thursday, October ... company will host a corresponding conference call for ... p.m. ET), during which management may discuss forward-looking ...
(Date:10/8/2015)... NXT-ID, Inc. (NASDAQ: ... authentication company focused on the growing mobile commerce ... announces that revenues for the three months ended ... $113,00 for the three months ended June 30, ... 30, 2015 were approximately $520,000.      ...
(Date:10/7/2015)... Connecticut , October 7, 2015 ... authentication company focused on the growing mobile commerce market ... Stanley E. Washington , former long- term executive ... of Directors. --> NXTD ) ("NXT-ID" or ... growing mobile commerce market and creator of the Wocket® ...
Breaking Biology News(10 mins):
(Date:10/12/2015)... cell surface marker detection market ... to a new report by Grand View Research, Inc. This ... of oncology diseases and other cell-associated disorders. --> ... USD 6.49 billion by 2022, according to a new report ... be attributed to rise in incidence of oncology diseases and ...
(Date:10/12/2015)...  Patara Pharma, a clinical-stage biotechnology company developing ... conditions, today announced the closing of a $26 ... the close of its sale of preferred stock, ... Agreement with Silicon Valley Bank whereby the Company ... use the funds from the financing to evaluate ...
(Date:10/12/2015)... , Belgium , Oct. 12, 2015 /PRNewswire/ ... that full results from a completed clinical study of its ... have been published in the online issue of Clinical ... The peer-reviewed study was conducted in collaboration with ... led by Roland Andersson , MD, PhD, Professor of ...
(Date:10/12/2015)... ... ... LifeTrak , a leader in consumer fitness, heart rate and activity ... tracker that seeks to meet the needs of multi-sport athletes. Zoom is the first ... both in water and on land, making it the only fitness tracker an athlete ...
Breaking Biology Technology: