Navigation Links
Protein-bait interactions, display libraries featured in Cold Spring Harbor Protocols
Date:3/1/2010

COLD SPRING HARBOR, N.Y. (Mon., Mar. 1, 2010) -- The use of recombinant proteins, antibodies, small molecules, or nucleic acids as affinity reagents is a simple yet powerful strategy to study the protein/bait interactions that drive biological processes. Analysis via mass spectrometry rather than western blotting extends the identification of interactors, often allowing detection of thousands of proteins from complex mixtures. But this increased sensitivity can lead to problems distinguishing specific interactions from background noise. In the March issue of Cold Spring Harbor Protocols (http://www.cshprotocols.org/TOCs/toc3_10.dtl), Shao-En Ong from the Broad Institute of MIT and Harvard (http://www.broadinstitute.org/proteomics/team.html) presents "Unbiased Identification of Protein-Bait Interactions Using Biochemical Enrichment and Quantitative Proteomics." This method uses quantitative proteomics approaches to compare enrichment with the bait of interest against samples using control baits to allow sensitive detection and discrimination of specific protein/bait interactions. As one of March's featured articles, it is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2010/3/pdb.prot5400).

The baculovirus expression vector system has been widely used to produce proteins originating from both prokaryotic and eukaryotic sources. It offers easy cloning techniques and abundant viral propagation, and since it is based on an insect cell environment, it provides eukaryotic posttranslational modification machinery. Surface modifications of the viral capsid enable specific targeting. Such modifications can be used to enhance viral binding and entry to a wide variety of both dividing and nondividing mammalian cells, as well as to produce antibodies against the displayed antigen. In addition, the technology should enable modifications of intracellular behavior, i.e., trafficking of recombinant "nanoparticles," a highly relevant feature for studies of targeted gene or protein delivery. In the March issue of Cold Spring Harbor Protocols (http://www.cshprotocols.org/TOCs/toc3_10.dtl), Christian Oker-Blom and colleagues (http://www.jyu.fi/bio/bit/tutkimus_files/research.html) provide a suite of articles detailing the use of baculovirus-based display and gene delivery systems. Their protocol for "Creation of Baculovirus Display Libraries" is featured, and is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2010/3/pdb.prot5393).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. GestureTek Unveils The CUBE V3 Portable, Plug and Play Interactive Projection Display System for Advertising and Exhibits
2. GestureTek Unveils The CUBE V3 Portable, Plug and Play Interactive Projection Display System for Advertising and Exhibits
3. Loyal alligators display the mating habits of birds
4. U.S. Navy Awards Lockheed Martin Six-year Contract Worth Up to $72 Million for Advanced Display Systems Support
5. Plants display molecular amnesia
6. Scripps Research scientists create new way to screen libraries of 10 million or more compounds
7. Using combinatorial libraries to engineer genetic circuits advances synthetic biology
8. Advances in cancer detection research by Virginia Tech engineer featured in British magazine
9. High-throughput genotyping, protein purification featured in Cold Spring Harbor Protocols
10. Vertical farming advocate to be featured at upcoming free lecture at NJIT
11. Methods for gene transfer in stem cells featured in Cold Spring Harbor Protocols
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/5/2017)... April 5, 2017  The Allen Institute for Cell ... Explorer: a one-of-a-kind portal and dynamic digital window into ... data, the first application of deep learning to create ... cell lines and a growing suite of powerful tools. ... these and future publicly available resources created and shared ...
(Date:3/30/2017)... LOS ANGELES , March 30, 2017  On ... Hack the Genome hackathon at ... This exciting two-day competition will focus on developing health ... experience. Hack the Genome is ... has been tremendous. The world,s largest companies in the ...
(Date:3/27/2017)... , March 27, 2017  Catholic Health ... and Management Systems Society (HIMSS) Analytics for achieving ... Adoption Model sm . In addition, CHS previously ... U.S. hospitals using an electronic medical record (EMR). ... its high level of EMR usage in an ...
Breaking Biology News(10 mins):
(Date:6/20/2017)... June 20, 2017  Kibow Biotech Inc., a pioneer ... the issuance of a new patent covering a unique ... the U.S. Patent and Trademark Office on May 23 ... the Buzz of Bio award in 2014 in ... developing non-drug approaches to chronic disease. Renadyl™, the first ...
(Date:6/20/2017)... (PRWEB) , ... June 20, 2017 , ... ... for discovery of antibody therapeutics from millions-diverse immune repertoires, announces launch of its ... San Diego, California. Dave Johnson, PhD, CEO of GigaGen, will present on Surge ...
(Date:6/19/2017)... , ... June 19, 2017 , ... Tunnell Consulting ... over 50 years. One of the biggest challenges faced by life sciences, biotech and ... affairs services team is Kati Abraham , who is well known in the ...
(Date:6/16/2017)... , ... June 16, 2017 , ... ... and analytics solutions, today announced that its Anzo Smart Data Lake® (Anzo ... Technology Solution’ category for the 2017 Software & Information Industry Association (SIIA) CODiE ...
Breaking Biology Technology: