Navigation Links
Protein-bait interactions, display libraries featured in Cold Spring Harbor Protocols
Date:3/1/2010

COLD SPRING HARBOR, N.Y. (Mon., Mar. 1, 2010) -- The use of recombinant proteins, antibodies, small molecules, or nucleic acids as affinity reagents is a simple yet powerful strategy to study the protein/bait interactions that drive biological processes. Analysis via mass spectrometry rather than western blotting extends the identification of interactors, often allowing detection of thousands of proteins from complex mixtures. But this increased sensitivity can lead to problems distinguishing specific interactions from background noise. In the March issue of Cold Spring Harbor Protocols (http://www.cshprotocols.org/TOCs/toc3_10.dtl), Shao-En Ong from the Broad Institute of MIT and Harvard (http://www.broadinstitute.org/proteomics/team.html) presents "Unbiased Identification of Protein-Bait Interactions Using Biochemical Enrichment and Quantitative Proteomics." This method uses quantitative proteomics approaches to compare enrichment with the bait of interest against samples using control baits to allow sensitive detection and discrimination of specific protein/bait interactions. As one of March's featured articles, it is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2010/3/pdb.prot5400).

The baculovirus expression vector system has been widely used to produce proteins originating from both prokaryotic and eukaryotic sources. It offers easy cloning techniques and abundant viral propagation, and since it is based on an insect cell environment, it provides eukaryotic posttranslational modification machinery. Surface modifications of the viral capsid enable specific targeting. Such modifications can be used to enhance viral binding and entry to a wide variety of both dividing and nondividing ma
'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Page: 1 2

Related biology news :

1. GestureTek Unveils The CUBE V3 Portable, Plug and Play Interactive Projection Display System for Advertising and Exhibits
2. GestureTek Unveils The CUBE V3 Portable, Plug and Play Interactive Projection Display System for Advertising and Exhibits
3. Loyal alligators display the mating habits of birds
4. U.S. Navy Awards Lockheed Martin Six-year Contract Worth Up to $72 Million for Advanced Display Systems Support
5. Plants display molecular amnesia
6. Scripps Research scientists create new way to screen libraries of 10 million or more compounds
7. Using combinatorial libraries to engineer genetic circuits advances synthetic biology
8. Advances in cancer detection research by Virginia Tech engineer featured in British magazine
9. High-throughput genotyping, protein purification featured in Cold Spring Harbor Protocols
10. Vertical farming advocate to be featured at upcoming free lecture at NJIT
11. Methods for gene transfer in stem cells featured in Cold Spring Harbor Protocols
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/31/2014)... developed a new test that can distinguish between birds ... avian influenza virus or "bird flu" with those that ... step in the fight against this often fatal strain ... populations of South East Asia, particularly Indonesia and Vietnam. ... transmission to humans and the threat of a pandemic ...
(Date:10/31/2014)... 30, 2014  HITLAB SM , a healthcare ... its inaugural HITLAB Innovators Summit SM on ... HITLAB will partner with the Clinton Foundation,s Health ... ProtoHack, Columbia Business School Alumni Club of ... Engineering and Operations Research, bringing together leaders in ...
(Date:10/30/2014)... has received a $1.04 million grant from the ... and reduce osteoarthritis using a patient,s own stem ... of biodegradable polymers., Liping Tang, a bioengineering professor ... the research is primarily focused on helping soldiers ... found that if we inject microscaffolding that we,ve ...
Breaking Biology News(10 mins):New step towards eradication of H5N1 bird flu 2HITLAB Announces Inaugural HITLAB Innovators Summit 2HITLAB Announces Inaugural HITLAB Innovators Summit 3UTA researcher uses microscaffolding injections to mend cartilage, prevent osteoarthritis 2
... like cancer typically destroy nasty malignant cells, while ... in synthetic biology, researchers are designing molecules intelligent ... cells alone. "We basically design molecules that actually ... of the cellular state before delivering the therapeutic ...
... 1996 marked the beginning of a revolution in biological and ... by now the number of characterised species is approaching 1000, ... a few years away mapping the genetic evolution of ... able to identify the genetic causes of human disease and ...
... , A mutual epidemiological relationship between aggressive periodontitis and ... at the universities of Kiel, Dresden, Amsterdam and Bonn have ... on chromosome 9, which maps to a genetic region that ... latest edition of the specialist journal PLoS Genetics . ...
Cached Biology News:Engineers create intelligent molecules that seek-and-destroy diseased cells 2Engineers create intelligent molecules that seek-and-destroy diseased cells 3Genetic differences between yeasts greater than those between humans and chimpanzees 2
(Date:10/31/2014)... Caustic soda is utilized in the production of zeolites, sodium ... lead in the global caustic soda market, followed by the ... in the USA, Germany, Brazil and Canada, is currently the ... of output and production capacities. , The global caustic soda ... year in the years ahead. The APAC region is the ...
(Date:10/31/2014)... Lawrence, MA (PRWEB) October 30, 2014 ... from Latin America were hosted by Charm Sciences ... October. During their week in the US, the ... chemical residue control, hygiene management, and process verification, ... state-of-the-art facility. , This was the first visit ...
(Date:10/31/2014)... (PRWEB) October 31, 2014 Following the ... a free learning webinar to introduce its new product ... directors and biostatisticians. , Launched in September, Formedix On ... suite of clinical trial automation tools. For the first ... web browser. And by introducing Formations – a revolutionary ...
(Date:10/31/2014)... The report "Dairy Alternative (Beverage) ... Flavored, Sweetened, Unsweetened), Channel (Supermarket, Health Store, Pharmacy, ... Forecast to 2018," defines and segments the dairy ... of revenue and volume for plant-based dairy alternative ... reach $14 Billion by 2018 with the Asia-Pacific ...
Breaking Biology Technology:China to Dominate Caustic Soda Market Through 2018, Says Merchant Research & Consulting in Its New Study 2Charm Sciences Hosts Executives from Latin America for Dairy Safety Executive Seminar 2Formedix to Introduce "Revolutionary" Clinical Trial Automation Software with Learning Webinar 2Dairy Alternative Beverage Market Projected to Reach $14 Billion by 2018 - Report by MarketsandMarkets 2Dairy Alternative Beverage Market Projected to Reach $14 Billion by 2018 - Report by MarketsandMarkets 3Dairy Alternative Beverage Market Projected to Reach $14 Billion by 2018 - Report by MarketsandMarkets 4
... are pleased to release the third in a ... , This third Podcast titled "Commercialization ... crucial issues around how nanotechnology can be taken ... commercial outcomes. , Dr Peter Binks, CEO of ...
... 7 Dendreon Corporation (Nasdaq:,DNDN) today announced that ... position of senior vice president of clinical affairs ... with over a decade of oncology,drug development and ... was instrumental to the filing of the company,s ...
... in the Department of Physics & Engineering ... with colleagues from the University of California, ... authored the article, High-frequency single-photon source with ... December 2007 issue of Nature Photonics ( ...
Cached Biology Technology:Commercialization of nanotechnology 2Dendreon Promotes Dr. Mark Frohlich to Senior Vice President of Clinical Affairs and Chief Medical Officer 2Dendreon Promotes Dr. Mark Frohlich to Senior Vice President of Clinical Affairs and Chief Medical Officer 3Professor Strauf's research is Nature Photonics’ cover article 2
... Ambion's custom siRNA service provides the premium ... silencing research. Custom siRNAs can be readily ... base target sequence, or by providing the ... out Ambion's Silencer Validated and Silencer Pre-designed ...
, Premium quality synthesis and purification , You provide the sequence, we'll do the rest , Rigorous quality control , Your choice of purification method, amount and format , Available annealed...
, Premium quality synthesis and purification , You provide the sequence, we'll do the rest , Rigorous quality control , Your choice of purification method, amount and format , Available annealed...
Human TGF-beta RIIb Affinity Purified Polyclonal Ab...
Biology Products: