Navigation Links
Protein analysis methods, viral vectors featured in Cold Spring Harbor Protocols

COLD SPRING HARBOR, N.Y. Many proteins do not function by themselves as stand-alone units. Instead, multiple proteins associate to form larger structures called protein complexes. The May issue of Cold Spring Harbor Protocols ( features a set of methods that can be used to analyze protein complexes. An additional featured article details the generation of viral vectors for gene transfer.

"Systematic Monitoring of Protein Complex Composition and Abundance by Blue-Native PAGE," written by Harvey Millar and colleagues from the University of Western Australia, describes multiple experimental approaches using polyacrylamide gel electrophoresis (PAGE). Blue-native PAGE (BN-PAGE) allows a range of protein complexes to be visualized. When combined with sodium dodecyl sulfate PAGE (SDS-PAGE), the procedure can resolve the complexes and their subunits by their molecular weight. In conjunction with differential in-gel electrophoresis (DIGE), BN-PAGE can be used to quantify changes in protein complex abundance or subunit composition between different samples. The article presents detailed methodology for BN-PAGE, SDS-PAGE, and DIGE. It is freely available on the website for Cold Spring Harbor Protocols.

Genetically modified adenoviruses serve as one of the most versatile and efficient gene delivery systems in use today. Laboratories throughout the world use adenoviruses for the delivery of DNA to cells for basic science and for gene therapy applications. Unlike most other vectors, adenoviruses can infect post-mitotic cells, which makes them particularly useful as vectors for gene delivery into cells like neurons. In the May issue of Cold Spring Harbor Protocols, Robin Parks and colleagues from the Ottawa Health Research Institute provide "Construction and Characterization of Adenovirus Vectors," a set of detailed instructions for the generation, propagation, purification, and characterization of adenovirus vectors. This method is freely accessible on the website for Cold Spring Harbor Protocols.


Contact: David Crotty
Cold Spring Harbor Laboratory

Related biology news :

1. Small molecules might block mutant protein production in Huntingtons disease
2. ISU researcher identifies key function in protein, cell transcription
3. Brain protein central to both Parkinsons, drug addiction identified
4. SUMO protein guides chromatin remodeler to suppress genes
5. New study reveals the protein that makes phosphate chains in yeast
6. Autopilot guides proteins in brain
7. New data on the breakdown of the KRas protein
8. HIV dearms protective protein in cells
9. UC Riverside biochemist to study how crops can increase protein production
10. Cloud computing brings cost of protein research down to Earth
11. Penn biologists discover how silent mutations influence protein production
Post Your Comments:
(Date:10/27/2015)... 27, 2015 In the present market scenario, ... for various industry verticals such as banking, healthcare, defense, ... growing demand for secure & simplified access control and ... as hacking of bank accounts, misuse of users, , ... as PC,s, laptops, and smartphones are expected to provide ...
(Date:10/26/2015)... , October 26, 2015 ... --> adds Biometrics Market ... 2021 as well as Emerging Biometrics ... reports to its collection of IT ... . --> ...
(Date:10/23/2015)... 23, 2015 Research and Markets ( ... Voice Recognition Biometrics Market 2015-2019" report to their ... The global voice recognition biometrics market to grow ... --> --> The report, ... based on an in-depth market analysis with inputs from ...
Breaking Biology News(10 mins):
(Date:12/1/2015)... , ... December 01, 2015 , ... Matthew “Tex” VerMilyea, ... post, VerMilyea will oversee all IVF lab procedures as well as continue ... preservation. , “We traveled 7,305 miles to Auckland, New Zealand to bring home a ...
(Date:11/30/2015)... ... November 30, 2015 , ... Global Stem Cells Group ... of a new closed system for isolating adipose-derived stem cells. The announcement starts a ... of adipose tissue. SVF is a component of the lipoaspirate obtained from liposuction of ...
(Date:11/30/2015)... , Dec. 1, 2015  An interventional radiology technique shows ... the preliminary results of a study being presented today at ... North America (RSNA). --> ... for decades by interventional radiologists as a way to stop ... procedure as a means of treating obesity is new. ...
(Date:11/30/2015)...  Culprits beware, a University at Albany research ... is taking crime scene fingerprint identification to a ... -->   --> Photo ... --> Halámek and his team ... concept for identifying whether a culprit is male ...
Breaking Biology Technology: