Navigation Links
Protein adaptation shows that life on early earth lived in a hot, acidic environment
Date:4/4/2011

sms and are essential for life in mammals, the researchers first constructed a family tree of the more than 200 thioredoxin sequences available from the three domains of life. Then they reconstructed the sequences of the ancestral thioredoxin enzymes using statistical methods based on maximum likelihood. Finally, they synthesized the genes that encoded these sequences, expressed the ancient proteins in the cells of modern Escherichia coli bacteria and then purified the proteins.

"By resurrecting proteins, we are able to gather valuable information about the adaptation of extinct forms of life to climatic, ecological and physiological alterations that cannot be uncovered through fossil record examinations," said Gaucher.

The reconstructed enzymes from the Precambrian period -- which ended about 542 million years ago -- were used to examine how environmental conditions, including pH and temperature, affected the evolution of the enzymes and their chemical mechanisms.

"Given the ancient origin of the reconstructed thioredoxin enzymes, with some of them predating the buildup of atmospheric oxygen, we thought their catalytic chemistry would be simple, but we found that thioredoxin enzymes use a complex mixture of chemical mechanisms that increases their efficiency over the simpler compounds that were available in early geochemistry," said Julio Fernndez, a professor in the Department of Biological Sciences professor at Columbia University.

Fernndez led a team that included Columbia University postdoctoral researchers Raul Perez-Jimenez, Jorge Alegre-Cebollada and Sergi Garcia-Manyes, and graduate student Pallav Kosuri in using an assay based on single molecule force spectroscopy to measure the activity level of the thioredoxin enzymes under different pH levels.

For their experiments, the researchers used an atomic force microscope to pick up and stretch an engineered protein in a solution containing thioredoxin. They first
'/>"/>

Contact: Abby Robinson
abby@innovate.gatech.edu
404-385-3364
Georgia Institute of Technology Research News
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Researchers identify new role for cilia protein in mitosis
2. Common chaperone protein found to work in surprising way, say Scripps Research scientists
3. Intelligent design: Engineered protein fragment blocks the AIDS virus from entering cells
4. HIV protein unveils vaccine target
5. Molecular muscle: Small parts of a big protein play key roles in building tissues
6. Mutant prions help cells foil harmful protein misfolding
7. Scripps Research team discovers new details about medically important protein family
8. Novel method could improve the performance of proteins used therapeutically
9. Synthetic biology: TUM researchers develop novel kind of fluorescent protein
10. UCLA engineers demonstrate use of proteins as raw material for biofuels, biorefining
11. Rockefeller Scientists discover new compound that rids cells of Alzheimer protein debris
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Protein adaptation shows that life on early earth lived in a hot, acidic environment
(Date:8/3/2015)... , Aug. 3, 2015 Synaptics, Inc. (Nasdaq: ... solutions, today announced that members of the executive management ... Pacific Crest Global Technology Leadership Forum on Tuesday, August ... will be held at the Sonnenalp Resort in ... presentation may include forward-looking information. An audio webcast of ...
(Date:7/31/2015)... Kina, 31. juli 2015 Den 10. internationale ... af BGI fra den 22. - 25. oktober i ... fejrer sin 10-års fødselsdag i år. Siden starten i ... årlige møder på ,omik,-feltet, og er en af de ... ICG-10 fokuserer på nylige gennembrud og fremskridt inden ...
(Date:7/31/2015)... 31 de julio de 2015 BGI llevará a ... ) del 22 al 25 de octubre de 2015, ... La conferencia celebra su décimo aniversario este año. Desde ... una de las reuniones anuales más influyentes del mundo ... de las conferencias científicas más dinámicas, entusiastas y amenas. ...
Breaking Biology News(10 mins):Den 10. internationale konference om Genomics (ICG-10) åbner i oktober 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 3
... A new research study at the University of Delaware ... microorganism that lives in seawater and is related to ... and virulence. V. parahaemolyticus is a ... with the consumption of raw or undercooked seafood, particularly ...
... dogs in western and central Africa comes from a ... ago, probably by European colonialists. In the current issue ... of scientists from Africa, USA and France report that ... country level and that there is only limited movement ...
... beneficial effect of anakinra for rheumatoid arthritis patients, but ... use with other biologic medications. Rheumatoid arthritis is ... in 100 people worldwide. It affects the joints, making ... new breed of arthritis drugs licensed in the past ...
Cached Biology News:UD research study to shed light on emerging seaborne pathogen 2UD research study to shed light on emerging seaborne pathogen 3Hope for a rabies eradication strategy in Africa 2Anakinra for rheumatoid arthritis: A modest benefit with some risk 2
(Date:8/4/2015)... ... August 04, 2015 , ... On August 4, 2015, the White House will ... from across the United States to talk about their experiences with innovation and present ... to be among the attendees. , One of the day’s aims is to grow ...
(Date:8/4/2015)... LOS ANGELES , Aug. 4, 2015   ... announced the recent acquisition of LifeLine Cryogenics, an east ... the New York tristate area. ... tissues, and all prepaid contracts will be honored. ... of cryogenics services, offering storage of sperm, eggs, embryos, ...
(Date:8/4/2015)... 4, 2015 /PRNewswire/ -- Sinovac Biotech Ltd. (Nasdaq: SVA ... China , today announced that it will ... ended June 30, 2015, after market close on Wednesday, ... on Thursday, August 13, 2015, at 8:00 a.m. EDT ... Time) to review the Company,s financial results and provide ...
(Date:8/3/2015)... 2015  Halozyme Therapeutics, Inc. (NASDAQ: HALO ) ... second quarter 2015 on Monday, August 10, 2015 at ... , president and chief executive officer, will lead the ... financial results for the second quarter 2015. ... section of Halozyme,s corporate website and a recording will ...
Breaking Biology Technology:White House Invites uBiome CEO Jessica Richman to First-Ever Startup Demo Day 2FamilyCord Announces Acquisition of LifeLine Cryogenics 2Sinovac to Host Conference Call to Report Second Quarter 2015 Unaudited Financial Results 2Halozyme to Host Second Quarter 2015 Financial Results Conference Call 2
... - The June 30 deadline is approaching for ... $223.9 million in benefits from a class action ... attorneys lawyers involved in the court-approved settlement. ... Wisconsin's antitrust and unfair competition laws. , ,The ...
... - Abandoned and failed information technology programs have cost state ... trend that has some members of the Legislature wondering if ... reprogram the whole mess. , ,There's probably no need to ... government's cut-and-paste IT operations. Instead, lawmakers should look for best ...
... - Since it was founded in 1998, EMSystem ... of web-based communications and resource management for hospitals and emergency ... attained that stature or gotten off the ground at all ... is, in the words of one of its founders, one ...
Cached Biology Technology:June 30 is deadline to claim up to $223.9M in Microsoft settlement 2Ethics Board site offers welcome contrast to state IT mistakes 2Ethics Board site offers welcome contrast to state IT mistakes 3Pre-eminent, web-based companies owe success to corporate venturing 2Pre-eminent, web-based companies owe success to corporate venturing 3Pre-eminent, web-based companies owe success to corporate venturing 4Pre-eminent, web-based companies owe success to corporate venturing 5Pre-eminent, web-based companies owe success to corporate venturing 6
Cells are grown in triple flasks or roller bottles. Viability and growth data are generated from a parallel representative culture....
... Agencourt offers SNP loci identification through ... automated assay design, intensive assay validation/optimization, ... of Corriell or customer-supplied samples and ... service provides for rapid and highly ...
... Agencourt offers pre-validated SNP ... 1500 amplicons within 93 ... kinases, and more than ... These assays were developed ...
... aqueous-based mounting medium designed for the ... is especially recommended when using tracers ... It can also be used with ... (FITC), Rhodamine (TMRITC) and Texas Red. ...
Biology Products: