Navigation Links
Properties of unusual virus revealed in research
Date:12/8/2008

A team of researchers from Penn State University and the University of Chicago has uncovered clues that may explain how and why a particular virus, called N4, injects an unusual substance -- an RNA polymerase protein -- into an E. coli bacterial cell. The results, which are published in the current issue of the journal Molecular Cell, contribute to improved understanding of the infection strategies used by viruses that attack bacterial cells. Such viruses are known as bacteriophages, or phages. The results also may help other researchers to come up with new ideas about ways to kill E. coli bacteria, which can be dangerous to humans.

"Most phages inject only their own DNA into bacterial cells," said Katsu Murakami, a Penn State assistant professor in the Department of Biochemistry and Molecular Biology and a leader of the study. "These phages then use the host bacterial cell's RNA polymerase to synthesize messenger RNA through a process called transcription, which ultimately results in the creation of new phage proteins. These new proteins are used to construct new phages inside the bacterial cell. But the phage that we are studying is different. It injects both its own DNA and its own RNA polymerase into bacterial cells, so it can begin the process of transcription without any help from the bacterial host's RNA polymerase."

The team says that the N4 phage that they are studying is the only phage that they know of that injects its own RNA polymerases into bacterial cells. "We are particularly interested in finding out why N4 injects its own RNA polymerase into bacterial cells and how the N4 RNA polymerase finds the N4 DNA and initiates transcription -- and, ultimately, the creation of new N4 phages -- once it is inside a bacterial cell," said Murakami.

To begin to answer these questions, team member Michael Gleghorn, a former graduate student in the Penn State Department of Biochemistry and Molecular Biology who is now a postdoctoral researcher at the University of Rochester, used X-ray crystallography to obtain a high-resolution three-dimensional image of the N4 phage's RNA-polymerase and DNA molecule. "By modifying the crystallography conditions, Michael obtained an extremely high-resolution picture of the N4 RNA polymerase and DNA molecule. So we are able to analyze protein-DNA interactions much more clearly," said Murakami.

The picture of this RNA polymerase and DNA molecule has enabled the team to investigate how the RNA polymerase initiates transcription of phage DNA from inside a bacterial cell. "When a phage injects its DNA into a bacterial cell, the amount of its DNA is miniscule compared to the amount of host DNA," said Murakami. "We wanted to find out what prevents the N4 RNA polymerase from binding to the bacterial host's DNA rather than to the phage's DNA."

It turns out that the N4 RNA polymerase is able to respond only to DNA that is shaped like a hairpin. Part of the N4 phage's DNA is shaped like a hairpin, whereas the E. coli bacterium's DNA is not shaped like a hairpin. Once the N4 RNA polymerase interacts with the phage's hairpin DNA, it begins to change its shape from a fisted form to a cupped form. By opening up, the RNA polymerase exposes its active site, which allows it to begin the transcription process.

While the researchers determined that the N4 RNA polymerase must change its form in order to bind to the phage DNA, they also found that this transformation isn't the polymerase's first as it progresses through the steps of phage infection. The team found that the polymerase must change form in order to squeeze through the phage's tiny injection tube as it is injected into the E. coli cell. "The diameter of the tube is narrower than the diameter of RNA polymerase," said Murakami. "This means that the enzyme must be unfolded into a longer and thinner structure in order to fit through the tube, and then it is refolded after it is injected into the cell."

The ability of the N4 RNA polymerase to withstand this unfolding and refolding is unique. Therefore, the team decided to experiment with this property by exposing the polymerase to high temperatures. As expected, the high temperatures caused the molecule to unfold. The scientists then cooled the molecule and watched as it reformed into its original shape and regained its functions.

In addition to helping scientists to advance their understanding of the process by which phages infect bacterial cells, Murakami hopes that the novel infection strategy of the N4 phage will be useful in the development of new therapeutic methods for killing E. coli. "The N4 virus injects its own RNA polymerase, which is a type of protein, into the E. coli cell. This system could be replicated and used to deliver proteins or drugs that kill the bacterium," said Murakami. This research was supported by the National Institutes of Health.


'/>"/>

Contact: Barbara K. Kennedy
science@psu.edu
814-863-4682
Penn State
Source:Eurekalert  

Related biology news :

1. Researchers study virus with unusual properties
2. Strained quantum dots show new optical properties
3. European researchers harness unique properties of boron to develop new drugs and diagnostics
4. UNC study on properties of carbon nanotubes, water could have wide-ranging implications
5. Researchers identify cancer preventive properties in common vitamin supplement
6. Environmental fate of nanoparticles depends on properties of water carrying them
7. Fast AFM probes measure multiple properties of biomolecules or materials simultaneously
8. Honeybee researcher to unravel properties governing lifespan with support from Norway
9. Videos extract mechanical properties of liquid-gel interfaces
10. Weird water: Discovery challenges long-held beliefs about waters special properties
11. TRAP preserves genetic properties of popular geranium
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Properties of unusual virus revealed in research
(Date:6/15/2016)... , June 15, 2016 ... report titled "Gesture Recognition Market by Application Market - Global Industry ... - 2024". According to the report, the  global gesture ... in 2015 and is estimated to grow at ... billion by 2024.  Increasing application of ...
(Date:6/2/2016)... The Department of Transport Management (DOTM) of ... Dollar project, for the , Supply and Delivery ... IT Infrastructure , to Decatur ... Identity Management Solutions. Numerous renowned international vendors participated in the ... was selected for the most compliant and innovative solution. The ...
(Date:5/20/2016)... May 20, 2016  VoiceIt is excited to ... VoicePass. By working together, VoiceIt and ... VoiceIt and VoicePass take slightly different approaches to ... both security and usability. ... this new partnership. "This marketing and ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... DIEGO , June 24, 2016 ... more sensitively detects cancers susceptible to PARP inhibitors ... circulating tumor cells (CTCs). The new test has ... HRD-targeted therapeutics in multiple cancer types. ... targeting DNA damage response pathways, including PARP, ATM, ...
(Date:6/23/2016)... Mass. , June 23, 2016   ... development of novel compounds designed to target cancer ... napabucasin, has been granted Orphan Drug Designation from ... the treatment of gastric cancer, including gastroesophageal junction ... stemness inhibitor designed to inhibit cancer stemness pathways ...
(Date:6/23/2016)... June 23, 2016  The Prostate Cancer Foundation (PCF) is pleased ... and faster cures for prostate cancer. Members of the Class of 2016 were ... Read More About the Class of 2016 PCF Young ... ... ...
(Date:6/23/2016)... ... June 23, 2016 , ... In a new case ... Denmark detail how a patient who developed lymphedema after being treated for breast cancer ... could change the paradigm for dealing with this debilitating, frequent side effect of cancer ...
Breaking Biology Technology: