Navigation Links
Preserved frogs hold clues to deadly pathogen
Date:6/20/2012

New Haven, Conn.A Yale graduate student has developed a novel means for charting the history of a pathogen deadly to amphibians worldwide.

Katy Richards-Hrdlicka, a doctoral candidate at the Yale School of Forestry & Environmental Studies, examined 164 preserved amphibians for the presence of Batrachochytrium dendrobatidis, or Bd, an infectious pathogen driving many species to extinction. The pathogen is found on every continent inhabited by amphibians and in more than 200 species. Bd causes chytridiomycosis, which is one of the most devastating infectious diseases to vertebrate wildlife.

Her paper, "Extracting the Amphibian Chytrid Fungus from Formalin-fixed Specimens," was published in the British Ecological Society's journal Methods in Ecology and Evolution and can be viewed at http://onlinelibrary.wiley.com/doi/10.1111/j.2041-210X.2012.00228.x/full.

Richards-Hrdlicka swabbed the skin of 10 species of amphibians dating back to 1963 and preserved in formalin at the Peabody Museum of Natural History. Those swabs were then analyzed for the presence of the deadly pathogen.

"I have long proposed that the millions of amphibians maintained in natural-history collections around the world are just waiting to be sampled," she said.

The samples were then analyzed using a highly sensitive molecular test called quantitative polymerase chain reaction (qPCR) that can detect Bd DNA, even from specimens originally fixed in formalin. Formalin has long been recognized as a potent chemical that destroys DNA.

"This advancement holds promise to uncover Bd's global or regional date and place of arrival, and it could also help determine if some of the recent extinctions or disappearances could be tied to Bd," said Richards-Hrdlicka. "Scientists will also be able to identify deeper molecular patterns of the pathogen, such as genetic changes and patterns relating to strain differences, virulence levels and its population genetics."

Richards-Hrdlicka found Bd in six specimens from Guilford, Conn., dating back to 1968, the earliest record of Bd in the Northeast. Four other animals from the 1960s were infected and came from Hamden, Litchfield and Woodbridge. From specimens collected in the 2000s, 27 infected with Bd came from Woodbridge and southern Connecticut. In other related work, she found that nearly 30 percent of amphibians in Connecticut today are infected, yet show no outward signs of infection.

Amphibian populations and species around the world are declining or disappearing as a result of land-use change, habitat loss, climate change and disease. The chytrid fungus, caused by Bd, suffocates amphibians by preventing them from respiring through their skin. Since Bd's identification in the late 1990s, there has been an intercontinental effort to document amphibian populations and species infected with it. Richards-Hrdlicka's work will enable researchers to look to the past for additional insight into this pathogen's impact.


'/>"/>
Contact: David DeFusco
david.defusco@yale.edu
203-436-4842
Yale University
Source:Eurekalert  

Related biology news :

1. Richer parasite diversity leads to healthier frogs, says University of Colorado study
2. Athletic frogs have faster-changing genomes
3. Clues to nervous system evolution found in nerve-less sponge
4. Higher pain tolerance in athletes may hold clues for pain management
5. Relative reference: Foxtail millet offers clues for assembling the switchgrass genome
6. Researchers look to relatives for clues in quest to develop sources of bioenergy
7. As deadly cat disease spreads nationally, MU veterinarian finds effective treatment
8. Researchers find critical regulator to tightly control deadly pulmonary fibrosis
9. Weakness can be an advantage in surviving deadly parasites, a new study shows
10. Nanotherapy: Treating deadly brain tumors by delivering big radiation with tiny tools
11. Common North American frog identified as carrier of deadly amphibian disease
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Preserved frogs hold clues to deadly pathogen
(Date:5/16/2017)... TEANECK, N.J. , May 16, 2017  Veratad ... leading provider of online age and identity verification solutions, ... the K(NO)W Identity Conference 2017, May 15 thru May ... Ronald Regan Building and International Trade Center. ... across the globe and in today,s quickly evolving digital ...
(Date:4/18/2017)... Inc., a global expert in SoC-based imaging and computing solutions, has ... features the company,s hybrid codec technology. A demonstration utilizing TeraFaces ® ... be showcased during the upcoming Medtec Japan at Tokyo Big Sight ... Las Vegas Convention Center April 24-27. ... Click here for an image of the ...
(Date:4/11/2017)... DUBLIN , Apr. 11, 2017 Research ... Tracking Market 2017-2021" report to their offering. ... The global eye tracking market to grow at ... The report, Global Eye Tracking Market 2017-2021, has been prepared based ... report covers the market landscape and its growth prospects over the ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... DuPont ... today that they have entered into a multiyear collaboration to identify and characterize ... with additional tools for gene editing across all applications. , Under the terms ...
(Date:10/12/2017)... (PRWEB) , ... October 12, ... ... ) has launched Rosalind™, the first-ever genomics analysis platform specifically designed for ... complexity. Named in honor of pioneering researcher Rosalind Franklin, who made a ...
(Date:10/11/2017)... CA, USA (PRWEB) , ... October 11, 2017 , ... ... to take place on 7th and 8th June 2018 in San Francisco, CA. The ... influencers as well as several distinguished CEOs, board directors and government officials from around ...
(Date:10/11/2017)... ... October 11, 2017 , ... Disappearing forests and increased emissions are the main ... people each year. Especially those living in larger cities are affected by air pollution ... of the most pollution-affected countries globally - decided to take action. , “I knew ...
Breaking Biology Technology: