Navigation Links
Predicting cancer prognosis
Date:8/27/2009

Stanford, CA Researchers led by Dr. Soheil Dadras at the Stanford University Medical Center have developed a novel methodology to extract microRNAs from cancer tissues. The related report by Ma et al, "Profiling and discovery of novel miRNAs from formalin-fixed paraffin-embedded melanoma and nodal specimens," appears in the September 2009 issue of the Journal of Molecular Diagnostics.

Cancer tissues from patients are often stored by a method that involves formalin fixation and paraffin embedding to retain morphological definition for identification; however, this method frequently prevents further molecular analysis of the tissue because of mRNA degradation. Even so, these tissues contain high numbers of microRNAs (miRNAs), which are short enough (~22 nucleotides) to not be broken down during the fixation process.

In this study, Dr. Dadras and colleagues optimized a new protocol for extracting miRNAs from formalin-fixed paraffin-embedded tissues. Using their new procedure, they identified 17 new and 53 known miRNAs from normal skin, melanoma, and sentinel lymph nodes. These miRNAs were well-preserved in a 10-year-old specimen. This new protocol, therefore, will allow for the identification of novel miRNAs that may differ in cancerous and healthy tissue, even from long-preserved tissue, leading to better predictions of disease prognosis and treatment response.

Ma et al suggest that their "cloning strategy has the advantage of not only discovering novel and known miRNA sequence identity but also providing an estimate of relative expression level. [This methodology may provide] a more robust strategy to obtain an accurate expression profile for novel and/or previously characterized small RNAs from clinically defined [formalin-fixed paraffin-embedded] tumor specimens, thereby facilitating the discovery of 'oncomirs' as biomarkers."


'/>"/>

Contact: Angela Colmone
acolmone@asip.org
301-634-7953
American Journal of Pathology
Source:Eurekalert

Page: 1

Related biology news :

1. Seeing the tree from the forest: Predicting the future of plant communities
2. Predicting risk of stroke from ones genetic blueprint
3. UBC study establishes formula for predicting climate change impact on salmon stocks
4. Predicting acute GVHD by gene expression could improve liver stem cell transplant outcomes
5. Predicting the distribution of creatures great and small
6. Predicting the perfect predator
7. Predicting the radiation risk to ESAs astronauts
8. Predicting growth hormone treatment success
9. Trial seeks genetic fingerprint for predicting drug effectiveness
10. U-Iowa improves delivery of cancer-fighting molecules
11. MicroRNA in human saliva may help diagnose oral cancer
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/19/2014)... improve students, chances of completing introductory biology courses ... (UH) implemented a comprehensive student success program, employing ... , Through a grant from the Texas ... Biology and Biochemistry embarked on a pilot program ... non-majors and "Introductory Biology" for science majors. These ...
(Date:8/19/2014)... Research and Network-Level Science, , Imagine if we had ... ecosystems was responding to global changes in real time. ... coupled multiple decades of information about ecological responses to ... dozens of different ecosystem types. , In fact, ... (LTER) Network, which will soon celebrate its 35th anniversary. ...
(Date:8/19/2014)... purchased at retail seafood counters in 10 different ... expose consumers to unexpectedly high levels of mercury, ... present a fish of the same species, but ... mislabeling offense, according to new research by University ... of seafood is essential to allow consumers to ...
Breaking Biology News(10 mins):University of Houson program earns kudos for improving grades, retaining students 2University of Houson program earns kudos for improving grades, retaining students 3This week from AGU: Long-term ecological research, predicting cholera outbreaks 2Seafood substitutions can expose consumers to unexpectedly high mercury 2Seafood substitutions can expose consumers to unexpectedly high mercury 3
... New Rochelle, NY, September 3, 2009Next-Generation Sequencing ... traditional Sanger sequencing methodology but are also giving ... well, reports Genetic Engineering & Biotechnology News ... workflow that eliminates transformation and colony picking and ...
... not be the first thing that comes to mind when ... the United States, South Korea and France, this might change. ... of a protein called "RanBP9" might be the key that ... them, hope. In a research report published online in The ...
... Results from the first swine-flu vaccine trials taking place in ... The pilot study, run by the University of Leicester ... between 18 and 50. Dr Iain Stephenson, who ... clinical trial of Novartis MF59-adjuvanted cell-based A (H1N1) vaccine indicates ...
Cached Biology News:GEN reports on expanding NextGen sequencing applications 2Scientists begin to untangle root cause of Alzheimer's disease 2World-first swine-flu vaccine trial reveals one dose provides 'strong immune response' 2
(Date:8/20/2014)... JOSE, Calif. , Aug. 20, 2014 /PRNewswire-iReach/ ... highlights the transformative collaboration between the school,s bioengineering ... As part of the Intel® Software Academic Program, ... computer sciences and the human body.  ... The study exhibits the work of Dr. ...
(Date:8/20/2014)... (PRWEB) August 20, 2014 ... released today including improvements to the enterprise replication ... connectors for Lucene, SOLR and Elasticsearch. This release ... this high performance triplestore – GraphDB™ was formerly ... in deploying the only mature enterprise resilient RDF ...
(Date:8/19/2014)... , Aug. 19, 2014 Research and Markets  has ... - Global Strategic Business Report" report to their offering. ... Tissue Culture Supplies in US$ Thousands by the following Product Segments: ... analytics for the US, Canada , ... , and Latin America . Annual estimates ...
(Date:8/19/2014)... 2014 Research and Markets  has announced the ... Global Strategic Business Report" report to their offering. ... Oxide Fuel Cells (SOFCs) in US$ Thousands. The report provides ... , Europe , Asia-Pacific ... provided for the period 2012 through 2020. Market data and ...
Breaking Biology Technology:Intel and University of San Diego Bioengineering Department Release Case Study on Health Sciences Research 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 3Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 4Global Cell and Tissue Culture Supplies - Strategic Business Report 2014 2Global Solid Oxide Fuel Cells (SOFCs) - Strategic Business Report 2014 2
... $8.1 Million Financing in Connection With Acquisition ... Reverse Stock Split Planned for January 2008, LONDON, ... that the Company has:, - Signed an agreement to acquire ... Israel. The acquisition,is expected to close tomorrow. The initial consideration is ...
... Calif., Dec. 4 Sangamo BioSciences,Inc. (Nasdaq: SGMO ... community on its latest developments in several of its,therapeutic ... and Analyst Briefing that will be held from 4:30 ... The presentation will be webcast live and may be ...
... - Associated Forum Provides Resources for Healthcare Professionals, ... ... 4 The Liko-sponsored Safe Lifting,Portal today announced the launch of ... associated safe lifting,forum, called "Safe Lifters," will allow healthcare professionals to,discuss ...
Cached Biology Technology:Amarin Signs Agreement to Acquire Ester Neurosciences 2Amarin Signs Agreement to Acquire Ester Neurosciences 3Amarin Signs Agreement to Acquire Ester Neurosciences 4Amarin Signs Agreement to Acquire Ester Neurosciences 5Amarin Signs Agreement to Acquire Ester Neurosciences 6Amarin Signs Agreement to Acquire Ester Neurosciences 7Amarin Signs Agreement to Acquire Ester Neurosciences 8Amarin Signs Agreement to Acquire Ester Neurosciences 9Amarin Signs Agreement to Acquire Ester Neurosciences 10Amarin Signs Agreement to Acquire Ester Neurosciences 11Sangamo BioSciences to Webcast Presentations at Investor and Analyst Briefing 2New Online 'Help Desk' Offers Safe Lifting Assistance to Healthcare Facilities 2
TEMPLIPHI HT SEQ AMPL KIT, 1 EA. Category: Sequencing Template Amplification....
This Malachite Green Phosphate Detection Kit...
Request Info...
1+ mg expected yield of purified protein, expressed in insect cells - excludes cDNA...
Biology Products: