COLD SPRING HARBOR, N.Y. Conventional approaches to proteomics have recently been augmented with a new generation of technologies unfamiliar to many life science researchers. A new methods book, Proteomics: Methods Express, identifies the most powerful of these new technologies and presents them in a way that allows their robust implementation in even non-specialist labs.
Proteomics: Methods Express is an essential, comprehensive laboratory manual and research guide for scientists in all biomedical fields and at all levels, from postgraduate student to principal investigator. It is published by Scion Publishing Ltd. (www.scionpublishing.com) and distributed in the United States, Canada, and South America by Cold Spring Harbor Laboratory Press (www.cshlpress.com).
The vast molecular diversity of proteins has triggered the development of a proportionately large number of proteomic approaches, write the editors, C. David OConnor and B. David Hames, in the Preface to the book. [I]t confuses novices, who need a core set of reliable protocols that are relatively painless to implement but sufficiently powerful to yield valuable new scientific information. This book provides such a set of procedures.
The chapters are presented in the order in which the methods are performed, starting with sample preparation and isotope-labeling. The next chapters present standard separation methods for proteins, peptides, and protein complexes, including gel electrophoresis, chromatography, and mass spectrometry. These are followed by chapters on modern, innovative procedures such as DESI (desorption electrospray ionization) and protein microarrays, as well as clinical applications for proteomic profiling and the analysis of post-translational modifications. The final chapters discuss bioinformatic-based approaches for handling the enormous amounts of data that are produced during such studies.
Each chapter includes a discussion of the merits and limitations of various approaches and then provides selected protocols with useful hints and tips for successand troubleshooting for when things go wrong. They include descriptions of what can, and currently cannot, be achieved with the relevant procedures so that readers can make informed judgments prior to establishing the methods in-house.
|Contact: Jane Carter|
Cold Spring Harbor Laboratory