Navigation Links
Polarized microscopy technique shows new details of how proteins are arranged
Date:4/17/2011

, graduate student Claire Atkinson and Martin Kampmann, a former a member of Gnter Blobel's Laboratory of Cell Biology who is currently at the University of California, San Francisco. It takes advantage of the properties of polarized light to show how specific proteins are aligned in relation to one another. After genetically attaching fluorescent markers to individual components of the nuclear pore complex, the scientists replaced the cell's own copy of the gene that encodes the protein with the new form that has the fluorescent tag. Then, they used customized microscopes to measure the orientation of the waves of light the fluorescently tagged proteins emitted. By combining these measurements with known data about the structure of the complex, the scientists can confirm or deny the accuracy of previously suggested models.

"Our experimental approach to the structure is synergistic with other studies being conducted at Rockefeller, including analysis with X-ray crystallography in Gnter's lab and electron microscopy and computer analysis in Mike Rout's lab," says Simon. "By utilizing multiple techniques, we are able to get a more precise picture of these complexes than has ever been possible before."

The scientists used the technique to study nuclear pore complexes in both budding yeast and human cells. In the case of the human cells, their new data shows that multiple copies of a key building block of the nuclear pore complex, the Y-shaped subcomplex, are arranged head-to-tail, rather than like fence posts, confirming a model proposed by Blobel in 2007.

"As a graduate student with Gnter Blobel, I determined the three-dimensional structure of the Y-shaped subcomplex using electron microscopy," says Kampmann. "However, it was still a mystery how these 'Y's are arranged. The new technique we have developed reveals the orientation of building blocks in the cell, and we hope that it will eventually enable us to assemble individual crystal stru
'/>"/>

Contact: Zach Veilleux
zveilleux@rockefeller.edu
212-327-8982
Rockefeller University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Polarized light guides cholera-carrying midges that contaminate water supplies
2. Next generation microscopy: No stain, big gain
3. Milestone in live microscopy focus of $2 million NIH grant
4. The sound of light: Innovative technology shatters the barriers of modern light microscopy
5. NIH grant will boost electron microscopy at Brandeis
6. New microscopy technique offers close-up, real-time view of cellular phenomena
7. New microscopy technique reveals mechanics of blood cell membranes
8. Boost for world-leading microscopy project
9. Faster CARS, less damage: NIST chemical microscopy shows potential for cell diagnostics
10. Imaging in depth: 3-dimensional microscopy featured in Cold Spring Harbor Protocols
11. New microscopy method opens window on previously unseen cell features
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/17/2014)... . To date, ... overdoses, doctors are often limited to supportive therapy such as ... combination of drugs involved. So what can be done if ... ETH professor Jean-Christophe Leroux from the Institute of Pharmaceutical Sciences ... question. "The task was to develop an agent that could ...
(Date:10/16/2014)... can be cultivated efficiently, they are anything but ... by monoculture cultivation is becoming increasingly evident. Despite ... form and are regarded as the sole possibility ... quite wrongfully, finds Bernhard Schmid, an ecology professor ... novel form of agriculture and forestry. After all, ...
(Date:10/15/2014)... As our ability to assess the pandemic risk from ... developments, we must not allow ourselves to become complacent ... an international consortium of scientists. , Influenza pandemics arise ... have yet to develop widespread immunity – spreads in ... in the past 100 years, the worst of which ...
Breaking Biology News(10 mins):Emergency aid for overdoses 2Emergency aid for overdoses 3Plant communities produce greater yield than monocultures 2Plant communities produce greater yield than monocultures 3Scientists 'must not become complacent' when assessing pandemic threat from flu viruses 2
... to perfumes is the most common contact allergy in ... that even natural aromatic oils, which many deem harmless ... Roughly one in five adults in northern Europe ... or more chemicals. The most common is nickel allergy, ...
... of Bioinformatics and the Swiss National Center of Competence ... a new test to predict how breast cancer patients ... is delivered in the future. In an article, ,A ... breast cancer, i, published in Nature Medicine , ...
... are abdominal obesity and high levels of a type of ... a person,s risk for stroke. The potential complications are serious: ... kidney disease and stroke. Preserving the ... a type of poly-unsaturated fat thought to play an important ...
Cached Biology News:Even natural perfumes may cause allergies 2Stroma genomic signature predicts resistance to anthracyclin-based chemotherapy in breast cancer 2Stroma genomic signature predicts resistance to anthracyclin-based chemotherapy in breast cancer 3Fighting obesity and illness with a novel approach to nutrition 2Fighting obesity and illness with a novel approach to nutrition 3
(Date:10/19/2014)... NextCODE Health, which enables clinicians and researchers to ... today announced the launch of its new genomic data analysis ... American Society of Human Genetics (ASHG) annual meeting in ... and apply for free beta access, visit www.nextcode.com . ... be viewed here . The Exchange: ...
(Date:10/19/2014)... Orthopedic braces and support systems report defines and segments the ... The Orthopedic braces and support systems market in Asia is ... a developing CAGR of 4.4% from 2013 to 2018. , ... support systems market, to get an idea of the in-depth ... of orthopedic braces and support systems market in the same ...
(Date:10/18/2014)... The report "1,6-Hexanediol Market by Application and ... analyzes the global market on the basis of ... details such as the key drivers, current trends ... Browse 81 Market Data Tables and 29 Figures ... “1,6-Hexanediol Market by Application and by Geography - ...
(Date:10/18/2014)... The Biomass Power industry ... years to 2014. The industry's expansion has been propelled ... that encouraged the use of biomass power. Furthermore, the ... which require local utilities to generate electricity from renewable ... Increased campaigning for green technology also influenced industry performance ...
Breaking Biology Technology:The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 2The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 3The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 2The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 3The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 41,6-Hexanediol Market Projected to Reach $916.1 Million at a CAGR of 8.3% by 2019 – Report by MarketsandMarkets 21,6-Hexanediol Market Projected to Reach $916.1 Million at a CAGR of 8.3% by 2019 – Report by MarketsandMarkets 31,6-Hexanediol Market Projected to Reach $916.1 Million at a CAGR of 8.3% by 2019 – Report by MarketsandMarkets 41,6-Hexanediol Market Projected to Reach $916.1 Million at a CAGR of 8.3% by 2019 – Report by MarketsandMarkets 5Biomass Power in the US Industry Market Research Report from IBISWorld Has Been Updated 2Biomass Power in the US Industry Market Research Report from IBISWorld Has Been Updated 3
... 9, 2011) The brain is a black box. ... much like the inner workings of a computer chip. ... economy of an assembly line, neural circuits are impenetrable ... Department of Neurobiology have developed a technique for unraveling ...
... of Standards and Technology (NIST),have demonstrated an ... mechanical component 1,000 times,more vigorously than ever ... a new tool for processing,information and potentially ... object at the,smallest possible, or quantum, scale. ...
... NEW YORK, March 9, 2011 Reportlinker.com ... is available in its catalogue: ... and Market Forecasts to 2017 ... Retinopathy Therapeutics - Pipeline Assessment and Market ...
Cached Biology Technology:Web-crawling the brain 2Web-crawling the brain 3NIST electromechanical circuit sets record beating microscopic 'drum' 2NIST electromechanical circuit sets record beating microscopic 'drum' 3Reportlinker Adds Diabetic Retinopathy Therapeutics - Pipeline Assessment and Market Forecasts to 2017 2Reportlinker Adds Diabetic Retinopathy Therapeutics - Pipeline Assessment and Market Forecasts to 2017 3
Mouse polyclonal antibody raised against a partial recombinant LASS3. NCBI Entrez Gene ID = 204219...
... SM-2 adsorbents are macroporous polystyrene beads for ... surface area for adsorbing organics of molecular ... The beads are useful for the adsorption ... for example, the removal of detergents such ...
MAb to Catenin beta, N-terminal (exon 2) Catenin, beta, N-terminal (exon 2)...
... Phosphate Phosphatase 2 (LPP2) ... aqueous solution Solution ... containing 0.08% sodium azide. ... the human LPP2 protein. ...
Biology Products: