Navigation Links
Polarized microscopy technique shows new details of how proteins are arranged
Date:4/17/2011

, graduate student Claire Atkinson and Martin Kampmann, a former a member of Gnter Blobel's Laboratory of Cell Biology who is currently at the University of California, San Francisco. It takes advantage of the properties of polarized light to show how specific proteins are aligned in relation to one another. After genetically attaching fluorescent markers to individual components of the nuclear pore complex, the scientists replaced the cell's own copy of the gene that encodes the protein with the new form that has the fluorescent tag. Then, they used customized microscopes to measure the orientation of the waves of light the fluorescently tagged proteins emitted. By combining these measurements with known data about the structure of the complex, the scientists can confirm or deny the accuracy of previously suggested models.

"Our experimental approach to the structure is synergistic with other studies being conducted at Rockefeller, including analysis with X-ray crystallography in Gnter's lab and electron microscopy and computer analysis in Mike Rout's lab," says Simon. "By utilizing multiple techniques, we are able to get a more precise picture of these complexes than has ever been possible before."

The scientists used the technique to study nuclear pore complexes in both budding yeast and human cells. In the case of the human cells, their new data shows that multiple copies of a key building block of the nuclear pore complex, the Y-shaped subcomplex, are arranged head-to-tail, rather than like fence posts, confirming a model proposed by Blobel in 2007.

"As a graduate student with Gnter Blobel, I determined the three-dimensional structure of the Y-shaped subcomplex using electron microscopy," says Kampmann. "However, it was still a mystery how these 'Y's are arranged. The new technique we have developed reveals the orientation of building blocks in the cell, and we hope that it will eventually enable us to assemble individual crystal stru
'/>"/>

Contact: Zach Veilleux
zveilleux@rockefeller.edu
212-327-8982
Rockefeller University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Polarized light guides cholera-carrying midges that contaminate water supplies
2. Next generation microscopy: No stain, big gain
3. Milestone in live microscopy focus of $2 million NIH grant
4. The sound of light: Innovative technology shatters the barriers of modern light microscopy
5. NIH grant will boost electron microscopy at Brandeis
6. New microscopy technique offers close-up, real-time view of cellular phenomena
7. New microscopy technique reveals mechanics of blood cell membranes
8. Boost for world-leading microscopy project
9. Faster CARS, less damage: NIST chemical microscopy shows potential for cell diagnostics
10. Imaging in depth: 3-dimensional microscopy featured in Cold Spring Harbor Protocols
11. New microscopy method opens window on previously unseen cell features
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/12/2015)... 2015 The mascots of the Atlantic Coast ... of Wake Forest Baptist Medical Center. They stopped by ... ACC Men,s Basketball Tournament. Young patients enjoyed bonding with ... their autographs. Brenner Children,s Hospital is the ... only children,s hospital in northwest North Carolina ...
(Date:3/11/2015)... BOSTON , March 11, 2015   ... of its platform to scientifically measure and harness ... characteristics to real-time biometrics and objective measurements of ... of music at scale in large populations. It ... scientists, technologists and clinicians perform rigorous studies and ...
(Date:3/10/2015)... OXFORD, Conn. , March 10, 2015 /PRNewswire/ ... NXTDW) ("NXT-ID" or the "Company"), a biometric authentication ... reports the company,s Wocket™ smart wallet has been ... 2015" by Rethink Modern. Rethink Modern ... sharing modern and innovative items for your home and lifestyle that ...
Breaking Biology News(10 mins):Introducing The Sync Project, a Global Collaboration Harnessing the Scientific Potential of Music for Health 2Introducing The Sync Project, a Global Collaboration Harnessing the Scientific Potential of Music for Health 3NXT-ID's Wocket Smart Wallet Named Number One Best Tech Gift for 2015 by Rethink Modern 2NXT-ID's Wocket Smart Wallet Named Number One Best Tech Gift for 2015 by Rethink Modern 3NXT-ID's Wocket Smart Wallet Named Number One Best Tech Gift for 2015 by Rethink Modern 4
... Mutations in a single gene can cause several ... considered different disorders. With support from the National ... exome sequencing a new gene scanning technology that ... mutations. "This is going to change the way ...
... Disease A new compound may offer an effective ... researchers from Brazil. They report their findings in the ... Chemotherapy . Chagas, disease is an infection caused by ... 18 million people and causes up to 50,000 deaths per ...
... , Global plant productivity that once was on the rise ... on the decline because of regional drought according to a ... is a measure of the rate of the photosynthesis process ... and water to sugar, oxygen and eventually plant tissue. Compared ...
Cached Biology News:Gene scan finds link across array of childhood brain disorders 2Gene scan finds link across array of childhood brain disorders 3Tips from the journals of the American Society for Microbiology 2Tips from the journals of the American Society for Microbiology 3Tips from the journals of the American Society for Microbiology 4Drought drives decade-long decline in plant growth 2
(Date:3/26/2015)... -- Neogen Corporation (NASDAQ: NEOG ) announced today ... fiscal 2015, which ended Feb. 28, increased 13% to ... per share in the current quarter were $0.20, compared ... increased 17% over prior year to $24,142,000, or $0.65 ... for the same period a year ago. ...
(Date:3/26/2015)... March 26, 2015 ReliantHeart, Inc., an ... Transonic, a supplier of precision transit-time flow measurement solutions, ... greatly improve the efficiency of the HeartAssist5® ... patients to experience greater mobility and peace of mind. ... miniature flow board, will draw 1/6th of the power ...
(Date:3/26/2015)... Ale Gicqueau, Founder and President of Clinovo, will co-present ... Technology, on “ Conducting Postmarket Registries - Rationale and ... and Exhibition on April 25th through April 28th in ... presentation, Ale Gicqueau and Victor Chen will discuss the ... requirements and resources to conduct the registry, as well ...
(Date:3/25/2015)... the most attractive in the emerging European region, mainly ... and increasing government investment in healthcare, according to a ... set to grow at twice the pace of the ... reaching an approximate market value of $43-60 billion by ... Cross provides total healthcare solutions that address the evolving ...
Breaking Biology Technology:Neogen reports 13% increase in net income 2Neogen reports 13% increase in net income 3Neogen reports 13% increase in net income 4Neogen reports 13% increase in net income 5Neogen reports 13% increase in net income 6Neogen reports 13% increase in net income 7Neogen reports 13% increase in net income 8LVAD patients to have greater mobility and peace of mind through Transonic and ReliantHeart’s True Flow collaboration for the HeartAssist5® 2LVAD patients to have greater mobility and peace of mind through Transonic and ReliantHeart’s True Flow collaboration for the HeartAssist5® 3Clinovo Presents at ACRP 2015 in Salt Lake City, Utah on April 25th-28th 2Clinovo Presents at ACRP 2015 in Salt Lake City, Utah on April 25th-28th 3
... bacteria, Duke University chemists and engineers have introduced a ... create microdevices such as labs-on-a-chip. , Their inkless microcontact ... nanometer, or billionths of a meter, the Duke team ... Journal of Organic Chemistry. , "This has a ...
... (Nasdaq:,SIAL) is pleased to announce that Patrick M. ... for the Research Biotech business unit, effective,October 1, ... Sigma-Aldrich,s,leadership position through the development of new and ... involved with Sigma-Aldrich,since 2005 as a member of ...
... BIOM ),(TSX: BRA) (the "Company") today announced that, ... in the United States, it intends,to change its name ... shareholder and court approval of a plan of arrangement, ... parent corporation,of a successor corporation of the current Biomira ...
Cached Biology Technology:Using catalysts to stamp nanopatterns without ink 2Using catalysts to stamp nanopatterns without ink 3Sigma-Aldrich Names Patrick Sullivan New VP of Research and Development for Research Biotech 2Biomira announces plan to change name to Oncothyreon 2Biomira announces plan to change name to Oncothyreon 3Biomira announces plan to change name to Oncothyreon 4Biomira announces plan to change name to Oncothyreon 5Biomira announces plan to change name to Oncothyreon 6
...
Handy 100V Power Supply is Low-cost, compact and lightweight. Easy to operate - either 50 or 100 VDC is selectable as the output voltage. These voltages are sufficient for ordinary electrophoresis....
... power supply (Cat. No. Minis 150VS) is ... meet most electrophoresis needs in a personal, ... 150V power supply is capable of running ... designed for individuals to have his/her own ...
BD BioCoat Collagen IV 35 mm Culture Dishes, tissue-culture treated polystyrene with a uniform application of mouse collagen type IV....
Biology Products: