Navigation Links
Plasma in bags
Date:11/11/2011

Physicians are increasingly using live cells in their treatments: in blood transfusions and bone marrow transplants, as well as in stem cell therapies and following severe burns. Cells taken from the patients themselves are ideal for replacing burned skin, eliminating immune deficiencies, repairing degenerated cartilage or to treat injured bones as they are not rejected by the immune system. These cells have to be kept, cultivated, reproduced or even modified in a patient-specific manner. The problem, however, lies in the storability of the cell solutions used. As they can easily become infected by germs, they can only be stored for a few days in the containers conventionally used today. The joint project InnoSurf aims to remedy this problem: scientists from five research institutions, along with partners in the industry have developed innovative plastic surfaces and measuring methods for efficiently producing human cells for diagnostic and therapeutic applications. The work was coordinated by the Helmholz Centre for Infection Research (HZI) in Braunschweig, Germany.

The idea is to cultivate the cells in sealed, sterile plastic bags. The inner surface of the bags has to be modified so that they provide cells with good conditions for survival. A team led by Dr. Michael Thomas at the Fraunhofer Institute for Surface Engineering and Thin Films IST in Braunschweig, Germany, has now developed a plasma technology process for use at atmospheric pressure. "We fill the bags with a specific gas mixture and apply an electrical voltage" explains scientific assistant Dr. Kristina Lachmann. "Inside them, for a brief period, plasma is created, i.e. a luminescent, ionized gas, which chemically alters the plastic surface". During this process the bag remains sterile as plasmas also have a disinfecting action. "The advantage of the process is that it operates at atmospheric pressure and is therefore cost-effective, fast and flexible" emphasizes group leader Dr. Michael Thomas who specializes in the use of such atmospheric pressure plasmas to modify surfaces.

The new bags facilitate the sterile handling of cell cultures. Previously, researchers and clinicians had to use open Petri dishes, bottles or bioreactors. As these systems need to be opened, at least for filling, contamination can easily occur. By contrast, when using the new technology with its sealed bag system, the cells migrate directly into the bag via an injection needle or connected tube systems without coming into contact with their surroundings. The sterile interior of the bags contains nutrient medium and germ-free air or a suitable gas, which been added beforehand. Even during the cultivation period the containers do not have to be opened, and at the end the cells can be removed again by injection needle.

The researchers also maybe intend to use the disposable systems for growing artificial organs. If the bags are provided with a three-dimensional structure, cells could attach themselves to it and create artificial skin, nerves, cartilage or bone which could be used prosthetically in the patient. So far their cultivation has mainly failed because the stem cells have been reluctant to attach themselves to spatial structures. The plasma process developed at the IST could solve this problem. In collaboration with the University of Tbingen, Braunschweig City Hospital plans to isolate certain stem cells from tissue samples and investigate on which of the new plastic surfaces they could develop into bone or cartilage, for example. For this development by the group led by Dr. Michael Thomas, the IST was awarded "Selected Location 2011" as part of the "Land of Ideas" initiative. The prize will be awarded at the IST in Braunschweig on December 8, 2011.


'/>"/>
Contact: Dr. Michael Thomas
michael.thomas@ist.fraunhofer.de
49-531-215-5525
Fraunhofer-Gesellschaft
Source:Eurekalert  

Related biology news :

1. RevaTen platelet-rich plasma shows promise as potential treatment for heart attacks
2. MIT researchers study the danger of toxoplasma parasites
3. Scripps Research scientists redefine the role of plasma cells in the immune system
4. Grant advances quark-gluon plasma studies

5. Atmospheric pressure plasma jet from a grounded electrode
6. Toxoplasma gondii spreads in the habitat of the Iberian lynx
7. Modeling Toxoplasma focus of workshop
8. Plasma produces KO cocktail for MRSA
9. How Toxoplasma gondii gets noticed
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Plasma in bags
(Date:3/22/2017)...   Neurotechnology , a provider of high-precision ... the release of the SentiVeillance 6.0 ... recognition using up to 10 surveillance, security and ... new version uses deep neural-network-based facial detection and ... a Graphing Processing Unit (GPU) for enhanced speed. ...
(Date:3/20/2017)... -- At this year,s CeBIT Chancellor Dr. Angela Merkel ... Chancellor came to the DERMALOG stand together with the Japanese Prime Minster ... country. At the largest German biometrics company the two government leaders could ... recognition as well as DERMALOG´s multi-biometrics system.   ... ...
(Date:3/13/2017)... , March 13, 2017 Future of security: Biometric Face ... ... DERMALOGs Face Matching enables to match face pictures against ... basis to identify individuals. (PRNewsFoto/Dermalog Identification Systems) ... DERMALOG,s "Face Matching" is the fastest software for biometric Face Matching ...
Breaking Biology News(10 mins):
(Date:3/24/2017)... NEW YORK , March 24, 2017 ... Composite ended the trading session at 5,817.69, down 0.07%; ... finish at 20,656.58; and the S&P 500 closed at ... session as 4 sectors closed in green, 4 sectors ... the day. This Friday, Stock-Callers.com has initiated reports coverage ...
(Date:3/23/2017)... ... 2017 , ... Lajollacooks4u is proud to announce it has become the premiere team-building cooking event ... companies around the world, such as Illumina, HP and Qualcomm, and is ranked #1 in ... popularity is due to its new team building format, a way for teams to not ...
(Date:3/23/2017)... LOUISVILLE, Colo. , March 23, 2017  GlobeImmune, ... purchase agreement for the sale of 12,835,490 shares of ... the  NantWorks  ecosystem of companies. In connection with the sale ... GlobeImmune $100,000 in cash and issue to GlobeImmune 200,000 ... common stock. "We are pleased to ...
(Date:3/23/2017)... YORK , March 23, 2017 ... ... of death, putting significant strain on health care systems, in ... cancer diagnoses rises, so too does the development of innovative ... minimum side effects. Among the many types of cancer treatments, ...
Breaking Biology Technology: