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Plants recognise pathogenic and beneficial microorganisms
Date:11/1/2012

mechanisms involved in distinguishing between Nod factor molecules secreted from rhizobia and chitin secreted by pathogenic microorganisms.

The challenging task of purifying plant receptor proteins, which are present in very low amounts in roots of the model legume Lotus japonicus, was successfully accomplished by expressing the receptors in heterologeous plant-based systems and purifying them from membrane fractions.

Another challenge was the establishment of binding assays with the carbohydrate ligands. Nod factor labelling and Nod factor immobilisation facilitated this, following application of chemoselective chemistry.

The researchers behind the results that have just been published in the international journal PNAS are affiliated with the Danish National Research Foundation's Centre for Carbohydrate Recognition and Signalling at the Department of Molecular Biology and Genetics, Aarhus University (Denmark), Department of Chemistry, University of Copenhagen (Denmark) and Department of Microbiology and Immunology, University of Otago (New Zealand).


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Contact: Jens Stougaard
stougaard@mb.au.dk
45-60-20-26-49
Aarhus University
Source:Eurekalert  

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