Navigation Links
Plants recognise pathogenic and beneficial microorganisms
Date:11/1/2012

Plant roots are surrounded by thousands of bacteria and fungi living in the soil and on the root surface. To survive in this diverse environment, plants employ sophisticated detection systems to distinguish pathogenic microorganisms from beneficial microorganisms.

Here the so-called chitin molecules from microorganisms, along with modified versions, play an important role as they are detected by the plant surveillance system. Legumes, for example, build a defence against pathogenic microorganisms in response to simple chitin molecules.

However, when the plant detects a specific modified chitin molecule (called a Nod factor) that is secreted from the rhizobium soil bacteria, formation of new organs in the form of "root nodules" occurs. Rhizobium bacteria are allowed to enter and colonise in these symbiotic organs, and they ultimately produce nitrogen for the plant.

The plants' detection of ligands such as chitin and modified Nod factors takes place through protein receptors that are localised on the surface of cells. Research at the Centre for Carbohydrate Recognition and Signalling (CARB) has now shown that ligand recognition through direct Nod factor binding is a key step in the receptor-mediated signal transduction that leads to root nodule development in legumes.

High-affinity binding was observed in the nano-molar range, comparable to the biologically relevant concentrations where Nod factor has in vivo activity. In contrast to this, simple chitin molecules bind to the receptors with low affinity. Structure-dependent ligand specificity and ligands binding affinities at different receptors may therefore determine which response mechanism is activated in plants exposed to different microbes or microbial communities in the environment.

Interdisciplinary approaches combining advanced biochemistry, chemoselective chemistry and microbial genetics made it possible to investigate the molecular mechanisms involved in distinguishing between Nod factor molecules secreted from rhizobia and chitin secreted by pathogenic microorganisms.

The challenging task of purifying plant receptor proteins, which are present in very low amounts in roots of the model legume Lotus japonicus, was successfully accomplished by expressing the receptors in heterologeous plant-based systems and purifying them from membrane fractions.

Another challenge was the establishment of binding assays with the carbohydrate ligands. Nod factor labelling and Nod factor immobilisation facilitated this, following application of chemoselective chemistry.

The researchers behind the results that have just been published in the international journal PNAS are affiliated with the Danish National Research Foundation's Centre for Carbohydrate Recognition and Signalling at the Department of Molecular Biology and Genetics, Aarhus University (Denmark), Department of Chemistry, University of Copenhagen (Denmark) and Department of Microbiology and Immunology, University of Otago (New Zealand).


'/>"/>

Contact: Jens Stougaard
stougaard@mb.au.dk
45-60-20-26-49
Aarhus University
Source:Eurekalert  

Related biology news :

1. E. coli adapts to colonize plants
2. Plants provide accurate low-cost alternative for diagnosis of West Nile Virus
3. Pitt biologist receives $2 million to study genetic diversity of plants worldwide
4. Scientists uncover mechanism by which plants inherit epigenetic modifications
5. New gene could lead to better bug-resistant plants
6. Researchers use banker plants to help battle whitefly pests
7. Gardeners delight offers glimpse into the evolution of flowering plants
8. No more sneezing, allergen-free house plants
9. No more sneezing, allergen free house plants
10. Plants fungi allies may not help store climate changes extra carbon
11. Plants unpack winter coats when days get shorter
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Plants recognise pathogenic and beneficial microorganisms
(Date:4/13/2017)... 13, 2017 According to a new market research ... Analytics, Identity Administration, and Authorization), Service, Authentication Type, Deployment Mode, Vertical, and ... is expected to grow from USD 14.30 Billion in 2017 to USD ... 17.3%. ... MarketsandMarkets Logo ...
(Date:4/6/2017)... , April 6, 2017 ... RFID, ANPR, Document Readers, by End-Use (Transportation & Logistics, ... Facility, Oil, Gas & Fossil Generation Facility, Nuclear Power), ... Educational, Other) Are you looking for a ... sector? ...
(Date:4/3/2017)... , April 3, 2017  Data ... precision engineering platform, detected a statistically significant ... product prior to treatment and objective response ... the potential to predict whether cancer patients ... to treatment, as well as to improve ...
Breaking Biology News(10 mins):
(Date:5/23/2017)... (PRWEB) , ... May 23, 2017 , ... ... for advanced technology applications, has announced a facility expansion to accommodate its rapid ... square feet of new workspace and renovation of the existing areas. The expansion ...
(Date:5/23/2017)... Boston, Massachusetts (PRWEB) , ... May 23, 2017 , ... ... is making a splash at this year’s Bio-IT World Conference and Expo ... Anzo Smart Data Lake® 4.0 solution. The Anzo Smart Data Lake is also ...
(Date:5/22/2017)... ... May 22, 2017 , ... Baltimore biotech firm, PathSensors, announced ... community in developing and issuing recommendations to grow Maryland's biohealth industry and position ... 2023. , The recommendations are contained in a report from ...
(Date:5/21/2017)... ... May 20, 2017 , ... CNSDose is ... trial and error process by finding the right antidepressant faster. CNSDose speeds ... doctor-patient relationship through a personalized approach to treatment. , A peer-reviewed ...
Breaking Biology Technology: