Plant roots are surrounded by thousands of bacteria and fungi living in the soil and on the root surface. To survive in this diverse environment, plants employ sophisticated detection systems to distinguish pathogenic microorganisms from beneficial microorganisms.
Here the so-called chitin molecules from microorganisms, along with modified versions, play an important role as they are detected by the plant surveillance system. Legumes, for example, build a defence against pathogenic microorganisms in response to simple chitin molecules.
However, when the plant detects a specific modified chitin molecule (called a Nod factor) that is secreted from the rhizobium soil bacteria, formation of new organs in the form of "root nodules" occurs. Rhizobium bacteria are allowed to enter and colonise in these symbiotic organs, and they ultimately produce nitrogen for the plant.
The plants' detection of ligands such as chitin and modified Nod factors takes place through protein receptors that are localised on the surface of cells. Research at the Centre for Carbohydrate Recognition and Signalling (CARB) has now shown that ligand recognition through direct Nod factor binding is a key step in the receptor-mediated signal transduction that leads to root nodule development in legumes.
High-affinity binding was observed in the nano-molar range, comparable to the biologically relevant concentrations where Nod factor has in vivo activity. In contrast to this, simple chitin molecules bind to the receptors with low affinity. Structure-dependent ligand specificity and ligands binding affinities at different receptors may therefore determine which response mechanism is activated in plants exposed to different microbes or microbial communities in the environment.
Interdisciplinary approaches combining advanced biochemistry, chemoselective chemistry and microbial genetics made it possible to investigate the molecular
|Contact: Jens Stougaard|