Navigation Links
Plants' oil-desaturating enzymes pair up to channel metabolites
Date:5/9/2014

s) of Arabidopsis, a common experimental plant.

Ying Lou, a postdoctoral research fellow working in Shanklin's lab, used several independent methods of bi-molecular complementation-methods that produce a signal if two test proteins come together-to establish which desaturases interact with themselves or others.

The scientists found that all the plant membrane desaturases they examined are capable of forming self-associating dimers in plant cells-pairings of two identical desaturase enzyme molecules. They also found that certain desaturases with different functions could also pair up, but others could not.

"The naturally pairing enzymes turn out to have interesting patterns," Shanklin said. "They are found in the same subcellular locations within the cell, and are involved in subsequent steps of the same metabolic pathway, suggesting a physiological driver for the observed pairings.

"Other pairings between very similar desaturases from different locations that we expected to pair up didn't," he added.

To test the idea that the paired enzymes were working together, the scientists conducted another series of experiments called metabolic flux analysis, drawing on Brookhaven biochemist Jorg Schwender's expertise. This method follows mass-labeled compounds through the various reaction pathways.

"Think of a city map with lots of ways to get from A to B. This method traces how many molecules travel along each route," Schwender said.

The analysis showed that one of the natural enzyme pairings performed two steps of a particular metabolic process without releasing an intermediate product.

"This was clear evidence that these two linked enzymes were working in concert to channel metabolites through this metabolic pathway in an efficient manner in living plant cells," Shanklin said. "Our findings suggest genetic techniques may be used to engineer these kinds of interactions into other desaturase enzymes-in
'/>"/>

Contact: Karen McNulty Walsh
kmcnulty@bnl.gov
631-344-8350
DOE/Brookhaven National Laboratory
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Light-sensitive eyes in plants
2. Putting the endoparasitic plants Apodanthaceae on the map
3. Plants send out signals attracting harmful bacteria, MU study finds
4. Plants with dormant seeds give rise to more species
5. Genetically modified tobacco plants as an alternative for producing bioethanol
6. Berkeley graduate student brings extinct plants to life
7. Tracking sugar movement in plants
8. New gel to promote bone growth on implants used in surgical procedures
9. Planning and building products and production plants simultaneously
10. First evidence of plants evolving weaponry to compete in the struggle for selection
11. Study finds forest corridors help plants disperse their seeds
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Plants' oil-desaturating enzymes pair up to channel metabolites
(Date:7/8/2015)... Florida , July 8, 2015 ... evolving as this summer,s must have products such as wearables, ... demand as popularity for biometrics based devices continues to rapidly ... NXT-ID, Inc. (NASDAQ: NXTD ), Google Inc. (NASDAQ: ... (NYSE: FIT ), GoPro, Inc. (NASDAQ: GPRO ) ...
(Date:7/7/2015)... , July 7, 2015  Based on ... Frost & Sullivan recognizes Credence ID, LLC with ... Entrepreneurial Company of the Year Award. Credence ID ... aid in its mission of offering enrollment and ... globally. As Credence ID was formed by experts ...
(Date:7/2/2015)... DUBLIN , June 25, 2015 ... has announced the addition of the "Natural ... Hybrid), Technologies (Recognition, IVR, OCR, Pattern & Image ... to their offering. The key vendors ... Google, HP, IBM Corporation, Microsoft Corporation, NetBase Solutions, ...
Breaking Biology News(10 mins):Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 2Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 3Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 4Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 5Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 6Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 2Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 3Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 4Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 5Natural Language Processing Market by Type (Rule-Based, Statistical, and Hybrid), Technologies (Recognition, IVR, OCR, Pattern & Image Recognition) - Worldwide Forecast to 2020 2
... Abnormalities in cardiac conduction, the rate at which ... are a major cause of death and disability around ... developing a new method, Electromechanical Wave Imaging (EWI), that ... activation of the heart. Based on ultrasound imaging, EWI ...
... to impair normal muscle function in rats, an ... according to Penn State researchers. "Our findings demonstrate ... and carrying excess weight," said Rudolf J. Schilder, ... Penn State College of Medicine. "We show that, ...
... Hopkins scientists have discovered how two closely related proteins ... attracting and repelling these axons as they navigate the ... to make remarkably precise connections. The discovery, reported ... that proteins belonging to the "semaphorin" family of guidance ...
Cached Biology News:Columbia engineers develop new method to diagnose heart arrhythmias 2Obesity creates wimpy rats 2Johns Hopkins scientists reveal nerve cells' navigation system 2Johns Hopkins scientists reveal nerve cells' navigation system 3
(Date:7/30/2015)... , July 30, 2015  Discovery Laboratories, ... it has received the second $1.0M tranche under ... Research (SBIR) grant valued at up to $3.0 ... aerosolized KL4 surfactant as a potential medical countermeasure ...  Discovery Labs was awarded an initial $1.0 million ...
(Date:7/29/2015)... July 30, 2015 According to a ... Growth, Trends & Forecasts (2015-2020), , published by Mordor Intelligence, ... billion by the end of 2020, with North ... more than 40% of the global market size. The Global ... CAGR of 13.7% during the period of (2015-2020). ...
(Date:7/29/2015)... 29, 2015  AsureQuality and Ubiquitome have agreed ... applications in food and primary production sectors. The ... PCR device, the Freedom4. AsureQuality provides ... the supermarket shelf for producers, processors and Competent ... animal disease control and pest management and surveillance ...
(Date:7/29/2015)... YORK , July 29, 2015  Therapath ... testing, announces increased partnerships with VA and Military ... Epidermal Nerve Fiber Density (ENFD) and Sweat Gland ... small fiber neuropathy ( https://therapath.com/skin ). ... Nerve Fiber Density (ENFD) on punch skin biopsy ...
Breaking Biology Technology:Discovery Labs Receives $1.0 million under an SBIR Grant in Continuing Support of the Development of Aerosolized KL4 Surfactant to Address Radiation-Induced Lung Injury 2Discovery Labs Receives $1.0 million under an SBIR Grant in Continuing Support of the Development of Aerosolized KL4 Surfactant to Address Radiation-Induced Lung Injury 3Asia Pacific to Dominate the Global Stem Cell Market - Vericel Corporation, Ocata Therpeutics, Athersys Inc., Cryo-Cell International, Geron Corporation, StemCells, Inc. Among the Major Players 2Asia Pacific to Dominate the Global Stem Cell Market - Vericel Corporation, Ocata Therpeutics, Athersys Inc., Cryo-Cell International, Geron Corporation, StemCells, Inc. Among the Major Players 3AsureQuality and Ubiquitome Partner on Applications for Mobile Molecular Food Safety Testing 2Neuropathology services for VA and Military health systems 2
... option for asthma patients not fully controlled on other ... ... Dey, L.P., an,affiliate of Merck KGaA of Darmstadt, Germany, announced ... partner, Critical Therapeutics, Inc. (Nasdaq: CRTX ). ZYFLO CR(TM),offers twice-daily, ...
... properties are shaped by ecological interactions. This is ... closely reflect what each species eats. However, for ... and developmental interactions may also influence species-specific properties. ... of Biotechnology show how development affects the evolution ...
... As Immunotec,looks to the beginning of its twelfth ... Mr. C.H. (Chuck) Roberts announces,his retirement from his ... Immunotec, will continue his involvement with the corporation, ... Directors and as Co-founder, in a variety,of public ...
Cached Biology Technology:Dey L.P. and Critical Therapeutics Launch Twice-Daily ZYFLO CR(TM) (Zileuton) Extended-Release Tablets for the Chronic Treatment of Asthma 2Dey L.P. and Critical Therapeutics Launch Twice-Daily ZYFLO CR(TM) (Zileuton) Extended-Release Tablets for the Chronic Treatment of Asthma 3Mice teeth explain the troubles with human wisdom teeth 2Immunotec Inc. announces retirement of President & CEO C.H. (Chuck) Roberts 2