Navigation Links
Plants' oil-desaturating enzymes pair up to channel metabolites
Date:5/9/2014

UPTON, NY-Plant scientists at the U.S. Department of Energy's Brookhaven National Laboratory have found that certain enzymes responsible for desaturating fatty acids, the building blocks of oils, can link up to efficiently pass intermediate products from one enzyme to another. "Engineering these enzyme interactions to channel metabolites along desired metabolic pathways could be a new approach for tailoring plants to produce useful products," said Brookhaven biochemist John Shanklin, lead author on a paper reporting the results in the Journal of Biological Chemistry.

Getting plants to accumulate high levels of more healthful polyunsaturated fatty acids, or unusual fatty acids that could be used as raw materials in place of petroleum-derived chemicals in industrial processes, are a few possible outcomes.

Metabolic channeling

The idea would be to take advantage of a process called metabolic channeling, wherein enzymes that act sequentially in a particular metabolic pathway interact with each other so that they are able to pass molecules to each other without them entering the general metabolite pool of the cell. This close arrangement of enzymes also prevents intermediates from entering the metabolic channel.

Previous studies by Shanklin's group had shown that a distinct kind of desaturase enzyme that floats freely in plant plastids-mini plant cell "organs" where many metabolic processes, including photosynthesis, take place-pair up with themselves to form structures called dimers. The group had also studied baker's yeast and determined that its membrane-bound desaturase formed dimers too. But no studies had looked for these kinds of macromolecular arrangements in membrane-bound desaturases, called FADs, in higher plants. The current study used a molecular-genetic approach to explore the organization of membrane desaturases found in the plastids and endoplasmic reticulum (another membrane structure inside cells) of Arabidopsis, a common experimental plant.

Ying Lou, a postdoctoral research fellow working in Shanklin's lab, used several independent methods of bi-molecular complementation-methods that produce a signal if two test proteins come together-to establish which desaturases interact with themselves or others.

The scientists found that all the plant membrane desaturases they examined are capable of forming self-associating dimers in plant cells-pairings of two identical desaturase enzyme molecules. They also found that certain desaturases with different functions could also pair up, but others could not.

"The naturally pairing enzymes turn out to have interesting patterns," Shanklin said. "They are found in the same subcellular locations within the cell, and are involved in subsequent steps of the same metabolic pathway, suggesting a physiological driver for the observed pairings.

"Other pairings between very similar desaturases from different locations that we expected to pair up didn't," he added.

To test the idea that the paired enzymes were working together, the scientists conducted another series of experiments called metabolic flux analysis, drawing on Brookhaven biochemist Jorg Schwender's expertise. This method follows mass-labeled compounds through the various reaction pathways.

"Think of a city map with lots of ways to get from A to B. This method traces how many molecules travel along each route," Schwender said.

The analysis showed that one of the natural enzyme pairings performed two steps of a particular metabolic process without releasing an intermediate product.

"This was clear evidence that these two linked enzymes were working in concert to channel metabolites through this metabolic pathway in an efficient manner in living plant cells," Shanklin said. "Our findings suggest genetic techniques may be used to engineer these kinds of interactions into other desaturase enzymes-including enzymes that don't associate naturally-to push metabolites along desired pathways to produce useful products."


'/>"/>

Contact: Karen McNulty Walsh
kmcnulty@bnl.gov
631-344-8350
DOE/Brookhaven National Laboratory
Source:Eurekalert  

Related biology news :

1. Light-sensitive eyes in plants
2. Putting the endoparasitic plants Apodanthaceae on the map
3. Plants send out signals attracting harmful bacteria, MU study finds
4. Plants with dormant seeds give rise to more species
5. Genetically modified tobacco plants as an alternative for producing bioethanol
6. Berkeley graduate student brings extinct plants to life
7. Tracking sugar movement in plants
8. New gel to promote bone growth on implants used in surgical procedures
9. Planning and building products and production plants simultaneously
10. First evidence of plants evolving weaponry to compete in the struggle for selection
11. Study finds forest corridors help plants disperse their seeds
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Plants' oil-desaturating enzymes pair up to channel metabolites
(Date:3/18/2016)... --> --> Competitive Landscape ... Vehicles, Physical infrastructure and Perimeter Surveillance & Detection Systems ... market and the continuing migration crisis in the ... has led visiongain to publish this unique report, which is ... & security companies in the border security market and ...
(Date:3/14/2016)... http://www.apimages.com ) - ... - Renvoi : image disponible via AP Images ( ... --> DERMALOG, le leader de l,innovation ... d,empreintes digitales pour l,enregistrement des réfugiés en Allemagne. ... produire des cartes d,identité aux réfugiés. DERMALOG dévoilera ...
(Date:3/9/2016)... , March 9, 2016 This BCC Research ... states of the RNA Sequencing (RNA Seq) market for ... as instruments, tools and reagents, data analysis, and services. ... segments of the RNA-Sequencing market such as RNA-Sequencing tools ... the main factors affecting each segment and forecast their ...
Breaking Biology News(10 mins):
(Date:5/20/2016)... CA (PRWEB) , ... May 20, 2016 , ... The ... 10 of its most experienced veterinary clients have treated over 100 of their own ... edge technology to provide the highest level of care for their patients. , ...
(Date:5/19/2016)... MAPLE RIDGE, British Columbia , May 19, ... of AdvanTec Global Innovations Inc. (AGI), based out ... recently added Greenlane Biogas Ltd. to its ... a 2-year contract manufacturing agreement. AFS along with ... Bending Technologies (ABT) is a vertically integrated industrial ...
(Date:5/18/2016)... Irvine, Calif. (PRWEB) , ... May 18, 2016 , ... ... had awarded the Luis Villalobos Award to Cognition Therapeutics at the annual ACA Summit ... innovative company that is financed by one of ACA’s member angel groups. It is ...
(Date:5/18/2016)... ... May 18, 2016 , ... The Academy of Model Aeronautics has ... dream of reaching a total of $1 million in awarded scholarships. , The AMA ... school graduates from across the nation has helped bring the total of AMA scholarships ...
Breaking Biology Technology: