Navigation Links
Plant Min protein sits tight and rescues E. coli
Date:5/24/2009

This release is available in Chinese.

A protein vital for correct chloroplast division in plants is able to take on a similar role in bacterial cells, according to research published today in the open access journal BMC Microbiology. The Arabidopsis thaliana Min protein (AtMinD) localizes in E. coli cells' polar regions keeping cell division at its correct central location, yet unlike its E. coli homologue, AtMinD does not oscillate.

Making certain that E. coli cells divide in the centre is down to Min proteins (MinC, D and E). MinE oscillates from the middle of the cell to one pole or another, driving the MinCD complex with it. The MinCD complex prevents FtsZ polymerization at the poles but not at the mid-line of the cell, where FtsZ ring formation leads to cell division.

A team of Beijing-based scientists expressed the Arabidopsis MinD gene (AtMinD), in E. coli cells that lacked the bacterial genes for both MinD and MinE. Surprisingly, the minicell phenotype of this E. coli HL1 mutant (MinDE) was rescued by the plant AtMinD gene, even though the dynamic MinE protein was absent. The Arabidopsis homologue AtMinD behaved differently from its E. coli counterpart in that it did not oscillate between poles, instead taking a stand at the pointed ends (puncta) of the poles of E. coli cells. The scientists went on to show that the rescue by plant AtMinD required E. coli MinC, and that AtMinD bound EcMinC in these puncta, This is another remarkable finding because while Arabidopsis (and other plants) encode plastid homologs of bacterial MinD and MinE, MinC is either absent or has diverged beyond recognition.

"The complementation of E. coli HL1 mutant (MinDE) by AtMinD and the requirement of EcMinC for this complementation suggest that the function of MinD is conserved between bacteria and plants," says Yikun He, a member of the research team. "However, this complementation doesn't require the presence of EcMinE suggesting that AtMinD may have some characters different from that of EcMinD."

Exactly why and how the AtMinD localizes to the polar region in E. coli cells is unknown, but one possibility is a mechanism similar to that found in Bacillus subtilis. In this bacterium, MinCD proteins are localized to polar regions without oscillation and there is no MinE. Instead another protein, known as DivIVA, tethers MinCD to cell poles, preventing division at the cell ends.

Chloroplasts originated from cyanobacteria that colonised primitive plant cells, and the conservation of MinD, MinE and FtsZ genes in plants was already an indication of some conservation of function. Nonetheless it is unexpected and exciting to find that plant MinD can collaborate with bacterial MinC to convert E. coli from an oscillating to a Bacillus-type mechanism of Min action, and this finding opens new avenues for exploring Min function in both bacteria and plants.


'/>"/>

Contact: Charlotte Webber
charlotte.webber@biomedcentral.com
44-782-531-7342
BioMed Central
Source:Eurekalert

Related biology news :

1. ISU researcher identifies genetic pathway responsible for much of plant growth
2. MIT’s implantable device offers continuous cancer monitoring
3. Bacteria create aquatic superbugs in waste treatment plants
4. Photoselective film proves effective for controlling height in potted gardenia plants
5. Fertilization intensifies competition for light and endangers plant diversity
6. International team finds key gene that allows plants to survive drought
7. Center to investigate plant cells for better biomass fuels
8. Plants could override climate change effects on wildfires
9. Plant gene mapping may lead to better biofuel production
10. Device protects transplanted pancreatic cells from the immune system
11. Tiny super-plant can clean up animal waste and be used for ethanol production
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/30/2017)... , March 30, 2017  On April 6-7, 2017, ... the Genome hackathon at Microsoft,s headquarters in ... competition will focus on developing health and wellness apps ... Hack the Genome is the first hackathon ... The world,s largest companies in the genomics, tech and ...
(Date:3/29/2017)... , March 29, 2017  higi, the health IT ... North America , today announced a ... the acquisition of EveryMove. The new investment and acquisition ... of tools to transform population health activities through the ... data. higi collects and secures data today ...
(Date:3/24/2017)... , March 24, 2017 The Controller General of ... Mr. Abdulla Algeen have received the prestigious international IAIR Award ... Continue Reading ... ... and Deputy Controller Abdulla Algeen (small picture on the right) have received ...
Breaking Biology News(10 mins):
(Date:10/7/2017)... ... October 06, 2017 , ... ... and applications consulting for microscopy and surface analysis, Nanoscience Instruments is now ... Analytical offers a broad range of contract analysis services for advanced applications. ...
(Date:10/7/2017)... , Oct. 6, 2017  The 2017 ... of three scientists, Jacques Dubochet, Joachim Frank ... developments in cryo-electron microscopy (cryo-EM) have ... within the structural biology community. The winners worked ... can now routinely produce highly resolved, three-dimensional images ...
(Date:10/6/2017)... ... October 06, 2017 , ... ... lunch discussion and webinar on INSIGhT, the first-ever adaptive clinical trial for glioblastoma ... Cancer Institute. The event is free and open to the public, but registration ...
(Date:10/5/2017)... ... , ... Understanding the microbiome, the millions of bacteria that live in our ... My Future, the newest exhibit on display at the University City Science Center’s Esther ... the lens of the gut microbiome. , Gut Love opens October 12, 2017, ...
Breaking Biology Technology: