Navigation Links
Pitt team recreates brain cell networks with new view of activity behind memory formation

PITTSBURGHUniversity of Pittsburgh researchers have reproduced the brain's complex electrical impulses onto models made of living brain cells that provide an unprecedented view of the neuron activity behind memory formation.

The team fashioned ring-shaped networks of brain cells that were not only capable of transmitting an electrical impulse, but also remained in a state of persistent activity associated with memory formation, said lead researcher Henry Zeringue [zuh-rang], a bioengineering professor in Pitt's Swanson School of Engineering. Magnetic resonance images have suggested that working memories are formed when the cortex, or outer layer of the brain, launches into extended electrical activity after the initial stimulus, Zeringue explained. But the brain's complex structure and the diminutive scale of neural networks mean that observing this activity in real time can be nearly impossible, he added.

The Pitt team, however, was able to generate and prolong this excited state in groups of 40 to 60 brain cells harvested from the hippocampus of ratsthe part of the brain associated with memory formation. In addition, the researchers produced the networks on glass slides that allowed them to observe the cells' interplay. The work was conducted in Zeringue's lab by Pitt bioengineering doctoral student Ashwin Vishwanathan, who most recently reported the work in the Royal Society of Chemistry (UK) journal, Lab on a Chip. Vishwanathan coauthored the paper with Zeringue and Guo-Qiang Bi, a neurobiology professor in Pitt's School of Medicine. The work was conducted through the Center for the Neural Basis of Cognition, which is jointly operated by Pitt and Carnegie Mellon University.

To produce the models, the Pitt team stamped adhesive proteins onto silicon discs. Once the proteins were cultured and dried, cultured hippocampus cells from embryonic rats were fused to the proteins and then given time to grow and connect to form a natural network. The researchers disabled the cells' inhibitory response and then excited the neurons with an electrical pulse.

Zeringue and his colleagues were able to sustain the resulting burst of network activity for up to what in neuronal time is 12 long seconds. Compared to the natural duration of .25 seconds at most, the model's 12 seconds permitted an extensive observation of how the neurons transmitted and held the electrical charge, Zeringue said.

Unraveling the mechanics of this network communication is key to understanding the cellular and molecular basis of memory creation, Zeringue said. The format developed at Pitt makes neural networks more accessible for experimentation. For instance, the team found that when activity in one neuron is suppressed, the others respond with greater excitement.

"We can look at neurons as individuals, but that doesn't reveal a lot," Zeringue said. "Neurons are more connected and interdependent than any other cell in the body. Just because we know how one neuron reacts to something, a whole network can react not only differently, but sometimes in the complete opposite manner predicted."

Zeringue will next work to understand the underlying factors that govern network communication and stimulation, such as the various electrical pathways between cells and the genetic makeup of individual cells.


Contact: Morgan Kelly
University of Pittsburgh

Related biology news :

1. CCNY-led interdisciplinary team recreates colonial hydrology
2. Penn biophysicists create new model for protein-cholesterol interactions in brain and muscle tissue
3. During exercise, the human brain shifts into high gear on alternative energy
4. Millisecond brain signals predict response to fast-acting antidepressant
5. Food for thought -- regulating energy supply to the brain during fasting
6. Risk and reward compete in brain
7. Brainy genes, not brawn, key to success on mussel beach
8. Brain-nourishing molecule may predict schizophrenia relapse
9. Brain structure provides key to unraveling function of bizarre dinosaur crests
10. MU brain imaging center provides research for autism, schizophrenia and Parkinsons disease
11. Key to function of dinosaur crests found in brain structure
Post Your Comments:
Related Image:
Pitt team recreates brain cell networks with new view of activity behind memory formation
(Date:11/17/2015)... 17, 2015  Vigilant Solutions announces today that Mr. ... of Directors. --> --> ... from the partnership at TPG Capital, one of the ... $140 Billion in revenue.  He founded and led TPG,s ... TPG companies, from 1997 to 2013.  In his first ...
(Date:11/12/2015)... CAMBRIDGE, Mass. , Nov. 12, 2015 /PRNewswire/ ... Broad Institute of MIT and Harvard for use ... chemical discovery information management tools. The partnership will ... share both biological and chemical research information internally ... tools will be used for managing the Institute,s ...
(Date:11/10/2015)... , Nov. 10, 2015 ... behavioral biometrics that helps to identify and verify ... Signature is considered as the secure and accurate ... identification of a particular individual because each individual,s ... accurate results especially when dynamic signature of an ...
Breaking Biology News(10 mins):
(Date:11/25/2015)... and HOLLISTON, Mass. , ... Inc. (Nasdaq: HART ), a biotechnology company developing ... CEO Jim McGorry will present at the ... 1, 2015 at 2:30 p.m. PT. The presentation will ... for 30 days. Management will also be available at ...
(Date:11/25/2015)... SAN DIEGO , Nov. 25, 2015 ... that management will participate in a fireside chat discussion ... New York . The discussion is ... Time. .  A replay will ... Contact:  Media Contact:McDavid Stilwell  , Julie NormartVP, Corporate ...
(Date:11/24/2015)... ... ... The United States Golf Association (USGA) today announced Dr. Bruce Clarke, of ... since 1961, the USGA Green Section Award recognizes an individual’s distinguished service to the ... of Iselin, N.J., is an extension specialist of turfgrass pathology in the department of ...
(Date:11/24/2015)... ... November 24, 2015 , ... Copper is ... it is bound to proteins, copper is also toxic to cells. With a ... Polytechnic Institute (WPI) will conduct a systematic study of copper in the bacteria ...
Breaking Biology Technology: