Navigation Links
Penn scientists pioneer new method for watching proteins fold
Date:12/23/2011

nd laser acts as the camera, following the motions of the protein's constituent amino acids.

"The protein is made of different groups of atoms, and the different groups can be thought of as springs," Gai said. "Each spring has a different frequency with which it moves back and forth, which is based on the mass of the atom on either end. If the mass is bigger, the spring oscillates slower. Our 'camera' can detect the speed of that motion and we can relate it to the atoms it is made of and how that segment of the protein chain moves."

Even in a simple protein like Trp-cage, however, there are many identical bonds, and the researchers need to be able to distinguish one from another in order to see which of them are moving while the protein folds. One strategy they used to get around this problem was to employ the molecular equivalent of a tracking device.

"We use an amino acid with a carbon isotope marker," Culik said. "If it's incorporated into the protein correctly, we'll know where it is."

With a single carbon atom of the Trp-cage slightly heavier than the others, the research team can use its signature to infer the position of the other atoms as they fold. The researchers could then "tune" the frequency of their laser to match different parts of the protein, allowing them to isolate them in their analyses.

Similar isotopes could be inserted in more complicated molecules, allowing their folds to also be viewed with infrared spectroscopy.

"This technique enhances our structural resolution. It allows us to see which part is moving," Gai said. "That would allow us to see exactly how a protein is misfolding in a disease, for example."


'/>"/>

Contact: Evan Lerner
elerner@upenn.edu
215-573-6604
University of Pennsylvania
Source:Eurekalert

Page: 1 2

Related biology news :

1. Jefferson scientists deliver toxic genes to effectively kill pancreatic cancer cells
2. Scientists identify novel inhibitor of human microRNA
3. Argonne scientists peer into heart of compound that may detect chemical, biological weapons
4. MU scientists go green with gold, distribute environmentally friendly nanoparticles
5. Scientists identify gene that may contribute to improved rice yield
6. Scientists discover why a mothers high-fat diet contributes to obesity in her children
7. MU scientists see how HIV matures into an infection
8. Earth scientists keep an eye on Texas
9. Thinking it through: Scientists call for policy to guide biofuels industry toward sustainability
10. Scientists identify a molecule that coordinates the movement of cells
11. Scientists Find new migratory patterns for Mediterranean and Western Atlantic bluefin tuna
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/22/2014)... of Yale researchers will lead a five-year, $3 million ... events is affecting the transport of dissolved organic matter ... say could alter the chemical composition and water quality ... funding from the National Science Foundation,s MacroSystems Biology program, ... the watershed, which begins in Canada and runs through ...
(Date:4/22/2014)... 22, 2014) Biotechnology scientists must be aware of ... and licensing guidelines, according to a new paper from ... in the current issue of the journal Regenerative ... 2013 U.S. Supreme Court ruling in the case Association ... occurring genes are unpatentable. The court case and rulings ...
(Date:4/22/2014)... University of Massachusetts Amherst and the Instituto Nacional de ... they have discovered a new genus and species of ... in the Amazonia State of Brazil. , Professor Cristina ... Jos Antnio Alves-Gomes of INPA, describe the new bluntnose ... Proceedings of the Natural Sciences of Philadelphia . ...
Breaking Biology News(10 mins):Connecticut River watershed study will assess impacts of extreme rain events 2Connecticut River watershed study will assess impacts of extreme rain events 3New patenting guidelines are needed for biotechnology 2New patenting guidelines are needed for biotechnology 3New electric fish genus and species discovered in Brazil's Rio Negro 2
... a $3 million five-year contract to Biomark Inc., of ... equipment to conduct on-going fish studies throughout the 17 ... endangered fish recovery programs. "This contract provides ... behavior that we rely upon to inform our river ...
... at the recent United Nations meeting emphasized nutrition as ... of State Hillary Clinton said a key focus is ... of Missouri nutrition experts say getting back to basics ... families and communities in many ways. As ...
... University,s Courant Institute of Mathematical Sciences, was today named ... highest honor bestowed by the United States government on ... of this year,s National Medal of Science will receive ... year. Varadhan, who has been recognized for his ...
Cached Biology News:Contract awarded for equipment to support on-going fish studies in the West 2Eating balanced meals, farm-fresh produce benefits families, communities, nutrition researchers say 2NYU mathematician Varadhan named recipient of National Medal of Science 2
(Date:1/14/2014)... DC (PRWEB) January 14, 2014 Carahsoft ... for Thursday, January 23, 2014 at 2pm EST (11am ... Knowledge.” The topic focuses on how technology can turn ... critical decisions for government agencies. The online webinar will ...
(Date:1/14/2014)... pet owners drew up their lists of New Year,s resolutions for ... of my furry companion." Nowadays there are pet salons, dog and ... take the little canine or feline darlings along wherever mommy or ... pricey toys at the pet store. But anyone who has ever ...
(Date:1/14/2014)... DIEGO and BETHESDA, Md. ... are joining together with two institutes from the National ... reliable tools for bringing safer, more effective treatments to ... the National Center for Advancing Translational Sciences (NCATS) and ...
(Date:1/14/2014)... 14, 2014  RXi Pharmaceuticals Corporation (OTCQX: RXII), a ... innovative therapies addressing major unmet medical needs using ... Notice of Allowance from the United States Patent ... compounds (sd-rxRNA®), for the treatment of fibrosis. The ...
Breaking Biology Technology:Webcast - Natural Language Processing: Converting Raw Data into Actionable Knowledge – Hosted by Carahsoft and CDS Federal Services 2New Year's Resolution: Give Pets the Gift of Top-Notch Health Care 2Organovo Announces Collaboration with National Institutes of Health 2Organovo Announces Collaboration with National Institutes of Health 3RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 2RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 3
... age rests on our ability to control how electric ... circuitry. Doing so requires particular precision, for applications ranging ... task falls to semiconductors. Now, a research team ... Applied Science and Arts and Sciences has shown how ...
... (Nasdaq: SGMO ) today announced the pricing of ... common stock at a price to the public of $7.70 ... or around Wednesday, April 13, 2011, subject to customary closing ... this offering and Sangamo has granted the underwriter a thirty ...
... Inc. (Nasdaq: SGMO ) announced today that it ... pursuant to an effective shelf registration statement in an underwritten ... in this offering and Sangamo will grant the underwriter a ... shares. The securities described above are being ...
Cached Biology Technology:Penn research advances understanding of lead selenide nanowires 2Penn research advances understanding of lead selenide nanowires 3Sangamo Announces Pricing of Public Offering of Common Stock 2Sangamo Announces Pricing of Public Offering of Common Stock 3Sangamo Announces Public Offering of Common Stock 2Sangamo Announces Public Offering of Common Stock 3
Mouse monoclonal [H4B4] to LAMP2 - Lysosome Marker (Phycoerythrin) ( Abpromise for all tested applications). entrezGeneID: 3920 SwissProtID: P13473...
... Nikon's NIS-Elements revolutionizes imaging software for ... intelligence to microscopes, cameras, components and ... and achiving tools. Its intuitive interface ... acquisition times while providing powerful features ...
AVOID FREEZE/THAW CYCLES. Recognizes the ~32 kDa DNase IIα chain and the ~40 kDa proenzyme. DNase II causes both DNA condensation and DNA fragmentation. ...
Vented Caps for 75 cm2 Flasks...
Biology Products: