Navigation Links
Penn biologists identify a key enzyme involved in protecting nerves from degeneration
Date:3/30/2012

arget and identified the wing of adult flies as a prime option.

The fly wing is not only translucent and a site of lengthy nerve fibers that can be easily observed, but it can also be cut to cause injury without killing the fly. That way, the researchers can follow the animal's response to nerve injury for weeks.

Using various reagents to manipulate the fly's genetic traits, the team confirmed that the cut wing nerve underwent Wallerian degeneration. They then tested versions of Nmnat and another protein called WldS, all of which had previously been shown to protect nerves from degeneration, to see if any of these might stop the process. All significantly delayed neurodegeneration. Even a form of Nmnat that hadn't worked in other animal models suppressed degeneration, although to a lesser extent.

"That indicates that our assay is really sensitive," Bonini said. "This sensitivity could help us identify genes that have moderate although important functionality at protecting against nerve degeneration."

Their investigations into the wing nerve also showed that the degenerating axon "died back," fragmenting first from the axon terminals, the side farthest from the nerve cell bodya pattern similar to what has been seen in other disorders.

Doing more genetic tinkering, the researchers showed that when the animal's own Nmnat was depleted, the nerves fragmented in the same way as if the axon was physically cut. And when Nmnat and the other "rescue" proteins were added back to these genetically modified flies, they were able to block degeneration, highlighting that Nmnat is critical to maintaining healthy axons.

In a final set of experiments, the biologists sought to narrow where in the nerve cells Nmnat might be working. They focused on mitochondria, the powerhouses of cells. When they created a genetic line of flies that blocked mitochondria from entering the axon fibers, the nerve tract degenerated, again, in a dyin
'/>"/>

Contact: Katherine Unger Baillie
kbaillie@upenn.edu
215-898-9194
University of Pennsylvania
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Caltech biologists spy on the secret inner life of a cell
2. Biologists, educators recognize excellence in evolution education
3. Caltech geobiologists discover unique magnetic death star fossil
4. Biologists discover gene behind plant sex mystery
5. Queens University biologists find new environmental threat in North American lakes
6. Biologists learn structure, mechanism of powerful molecular motor in virus
7. Biologists discover link between CGG repeats in DNA and neurological disorders
8. Biologists solve mystery of black wolves
9. Cleaning up oil spills can kill more fish than spills themselves, say Queens biologists
10. Plant biologists discover gene that switches on essence of male
11. Two innovative University of Texas at Austin biologists become HHMI Early Career Scientists
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/28/2014)... Clinic researcher and his collaborators have developed an online ... process of re-engineering cells for biomedical investigation. CellNet is ... to aid stem cell engineering. Details of CellNet and ... two back-to-back papers in the journal Cell . ... uses for all types of cell-based investigations and can ...
(Date:8/28/2014)... MD FASEB MARC (Maximizing Access to Research ... for the Genetics Society of America,s 27th Annual ... October 3, 2014 in Pacific Grove, California. ... of students, post doctorates and scientists from underrepresented ... community and to encourage the participation of young ...
(Date:8/28/2014)... -- Scientists have identified the developmental on-off switch for ... produce more than two-thirds of the world,s naturally derived ... to see whether it is possible to manipulate this ... , The study, appearing August 28 in Cell ... molecule called cyclic-di-GMP and a larger protein called BldD ...
Breaking Biology News(10 mins):New tool aids stem cell engineering for medical research 2Small molecule acts as on-off switch for nature's antibiotic factory 2Small molecule acts as on-off switch for nature's antibiotic factory 3
... The World Science Festival launches its first full day with ... will feature a keynote address by Harold Varmus, co-chair of ... well as a live simulcast of the announcement of the ... Oslo, Norway. A panel discussion follows, moderated by Elizabeth ...
... (May 14, 2010)--The New York Stem Cell Foundation (NYSCF) ... Robertson Foundation, established by Julian and Josie Robertson. ... will provide funding for NYSCF-Robertson Investigators in NYSCF,s new ... the NYSCF-Robertson Stem Cell Prize. This award ...
... 2010 The same digital technology that enables young people ... immerse them in a simulated coral reef environment where they ... project led by Field Museum scientists will link students from ... reef environments in the Pacific Ocean, engage in the scientific ...
Cached Biology News:Field Museum technology project wins prestigious award 2
(Date:8/28/2014)... Ca. (PRWEB) August 28, 2014 Best ... rating for an alcohol-based hand sanitizer, asks food processors ... by comparing the hand sanitizer they’re currently using to ... . Hand hygiene is critical to fighting cross-contamination and ... Best Sanitizers believes there are key criteria that ...
(Date:8/28/2014)... 28, 2014 The ability for organizations ... that will allow individuals to perform at the highest ... for employers looking to compete in this post–recession era. ... this trend, as the sector increasingly focuses on the ... which will have real results on the organization's development ...
(Date:8/28/2014)... TALLAHASSEE, Fla. Exciting new work by a Florida ... molecular system that can take your temperature, emit white ... , And, the molecule looks like a butterfly. , ... and Biomedical Engineering in the FAMU-FSU College of Engineering, ... ago, but has continued to discover that his creation ...
(Date:8/28/2014)... San Diego, CA (PRWEB) August 28, 2014 ... products, Pipette.com is offering a promotion for ... enables Pipette.com customers to purchase the highest quality ... Centrifuge Tubes are ideal for customers doing chemical, ... benefit lab technicians who are not regularly able ...
Breaking Biology Technology:Food Processors and Food Handlers are Encouraged to Prepare for Fall Harvest by Evaluating Their Current Hand Sanitizer Using Nine Key Criteria 2Food Processors and Food Handlers are Encouraged to Prepare for Fall Harvest by Evaluating Their Current Hand Sanitizer Using Nine Key Criteria 3Klein Hersh International Leads Philadelphia Area Life Science Industry in Company Culture 2Klein Hersh International Leads Philadelphia Area Life Science Industry in Company Culture 3Novel 'butterfly' molecule could build new sensors, photoenergy conversion devices 2Eppendorf Safe Lock Centrifuge Tube Promotion Now Available at Major Pipette Distributor Pipette.com 2Eppendorf Safe Lock Centrifuge Tube Promotion Now Available at Major Pipette Distributor Pipette.com 3
... Veteran healthcare executive brings solid experience with international ... healthcare and technology companies, ... management tools, announced the appointment of,Guillermo Moreno as its vice ... for developing and implementing strategic,initiatives to grow the company,s business ...
... Corporation,(Nasdaq: SIAL ) will be presenting at the ... at 8:30 AM ET at the Grand Hyatt in,New ... broadcast over the,Internet available at http://ir.sigmaaldrich.com . Users ... the webcast on, http://ir.sigmaaldrich.com users will need to ...
... FREMONT, Calif., Sept. 17 Quark ... and developing novel,RNA interference (RNAi)-based therapeutics, ... the 5th Annual RNAi Europe, taking ... Three of Quark,s scientists,Igor Mett, Hagar ...
Cached Biology Technology:Guillermo Moreno Named Aurillion's Vice President of Sales and Marketing 2Quark Pharmaceuticals Announces Poster Presentations at RNAi Europe 2008 2Quark Pharmaceuticals Announces Poster Presentations at RNAi Europe 2008 3
... Isothermal Block is an anodized aluminum, solvent-resistant ... and to provide efficient thermal transfer to ... design of the HT96 Isothermal Block replaces ... product design allowing for compatibility with robotic ...
Human monocyte-derived dendritic cells, For immunohistochemistry (IHC) Cell Chip with Human Peripheral Blood Dendritic Cells and EPCs...
...
...
Biology Products: