Navigation Links
Penn biologists discover how 'silent' mutations influence protein production
Date:4/9/2009

Sciences, and colleagues at Harvard University and the University of Edinburgh engineered a synthetic library of 154 genes that varied randomly at synonymous sites. All the genes encoded the same green fluorescent protein, enabling the researchers to easily study the effects of such mutations on protein levels when expressed in the bacterium Escherichia coli.

The silent mutations changed the amount of fluorescent protein by as much as 250-fold, without changing the properties of the protein. Codon bias, the probability that one codon of three adjacent nucleotides will code for one amino acid over another, was previously thought to be the cause for protein expression variance, but it did not correlate with gene expression in these experiments.

"At first we were stumped," Plotkin said. "How were the silent mutations influencing protein levels? Eventually, we looked at mRNA structure and discovered that this was the underlying mechanism."

The stability of mRNA folding near the ribosomal binding site explained more than half the variation in protein levels. To understand this observation, the researchers simulated the spatial arrangement of the messenger RNA molecule that carries the information from genes to proteins. They found that the inefficient genes produced tightly folded mRNA molecules that could not be accessed by the protein-making machinery. According to their analysis, mRNA folding and associated rates of translation initiation play a predominant role in shaping expression levels of individual genes, whereas codon bias influences global translation efficiency and cellular fitness.


'/>"/>

Contact: Jordan Reese
jreese@upenn.edu
215-573-6604
University of Pennsylvania
Source:Eurekalert  

Page: 1 2

Related biology news :

1. UIC biologists use DNA to study migration of threatened whale sharks
2. Penn biologists demonstrate that size matters... in snail shells
3. Two innovative University of Texas at Austin biologists become HHMI Early Career Scientists
4. Plant biologists discover gene that switches on essence of male
5. Cleaning up oil spills can kill more fish than spills themselves, say Queens biologists
6. Biologists solve mystery of black wolves
7. Biologists discover link between CGG repeats in DNA and neurological disorders
8. Biologists learn structure, mechanism of powerful molecular motor in virus
9. Queens University biologists find new environmental threat in North American lakes
10. Biologists discover gene behind plant sex mystery
11. Caltech geobiologists discover unique magnetic death star fossil
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Penn biologists discover how 'silent' mutations influence protein production
(Date:9/2/2014)... Air pollution regulations over the last decade in Taiyuan, ... there, accounting for a greater than 50% reduction in ... 2001 and 2010, according to researchers at the Columbia ... School of Public Health, the Shanxi Medical University, the ... and Shanghai Fudan University School of Public Health. , ...
(Date:9/2/2014)... Bavaria, the Tithonian Konservat-Lagersttte of lithographic limestone is ... of emblematic fossils from that area (for example, ... found fossil insects in the French equivalent of ... species representing the oldest known water treader. , ... Bavarian outcrops, fewer fossils have been obtained from ...
(Date:9/2/2014)... others are done in by it. New research at ... so-called stress gap in mice with very similar genetic ... better understand the development of psychiatric disorders such as ... unique experiences as it goes through its life. And ... expression of genes, and as a result, affect an ...
Breaking Biology News(10 mins):Clean air halves health costs in Chinese city 2Exceptionally well preserved insect fossils from the Rhône Valley 2Discovery hints at why stress is more devastating for some 2Discovery hints at why stress is more devastating for some 3
... In April 2010, the US government adopted a new nuclear ... defense systems will reliably protect the continental United States in ... Bulletin of Atomic Scientists , published by SAGE, shows ... and would not be effective in real combat conditions. ...
... the human immune system,s assassin a protein called perforin ... funded by the Biotechnology and Biological Sciences Research Council (BBSRC) ... where they used powerful electron microscopes to study the mechanism ... research is published today (1800hrs, 31 October) in Nature ...
... have discovered a new member of a gene family that ... weight. The gene is the third member of the agouti ... One helps determine skin and hair color, and the other ... new gene, called agrp2, has been found exclusively in bony ...
Cached Biology News:US nuclear safety claim is a 'dangerous fantasy' 2US nuclear safety claim is a 'dangerous fantasy' 3Immune system assassin's tricks visualised for the first time 2Newly discovered gene enables fish to 'disappear' 2
(Date:9/2/2014)... ON , Sept. 2, 2014 /PRNewswire/ - BIOREM Inc. (TSXV: ... totalling $2.1 million. The orders consist of three large odour ... Canada . "These orders are consistent with ... said Peter Bruijns , President and CEO. "After a ... market, we are now beginning to see sales growth. Our ...
(Date:9/1/2014)... NEW YORK , Sept. 1, 2014 /PRNewswire/ ... report is available in its catalogue: ... Opportunities, 2019 http://www.reportlinker.com/p02350445/India-Solid-Waste-Management-Vehicles-Market-Forecast-and-Opportunities-2019.html ... municipal corporations are the main governing bodies responsible ... management in cities. On an average, around 135,000 ...
(Date:8/31/2014)... Dallas, Texas (PRWEB) August 31, 2014 ... Global Polyacrylamide Industry” is a professional and ... The report provides basic Polyacrylamide information, including ... structure as well as industry overview. This ... domestic market as well as global industry ...
(Date:8/29/2014)... August 29, 2014 The global ... billion in 2019. It is expected to grow ... 2019, and was valued at $1.8 billion in ... Transparency Market Research. , For more information regarding ... . , The research report, titled “Companion Diagnostics ...
Breaking Biology Technology:India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 2India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 3India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 4India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 5India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 6India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 7India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 8International Polyacrylamide Industry & Copper Iodide Market Data Analysis Now Available at DeepResearchReports.com 2International Polyacrylamide Industry & Copper Iodide Market Data Analysis Now Available at DeepResearchReports.com 3Companion Diagnostics Market to Hit $5.6 Billion in 2019: Transparency Market Research 2Companion Diagnostics Market to Hit $5.6 Billion in 2019: Transparency Market Research 3
... Biotech and pharma,companies are increasingly relying on ... Engineering & Biotechnology News (GEN). The,technique can provide ... drug,candidates for toxic effects, according to an article ... )., "Better screening technologies, noninvasive monitoring, and ...
... /PRNewswire-FirstCall/ - Oncothyreon Inc.,(Nasdaq: ONTY ) (TSX:ONY) ... and preclinical studies of PX-866 were presented at ... Research (AACR) in,San Diego, held April 12-16, 2008. ... plans for these product candidates. Oncothyreon,is currently conducting ...
... Anavex Life,Sciences Corp. ("ANAVEX") (OTCBB: AVXL) today announced ... of Clinical Development as the,company prepares to enter ... the next 12 months., Dr. Goundis has ... of,clinical trials, including leadership positions at Speedel AG ...
Cached Biology Technology:Genetic Engineering & Biotechnology News Reports on Growing Use of Cell-Based Assays 2Data for Oncothyreon's PX-12 and PX-866 presented at AACR Annual Meeting 2Data for Oncothyreon's PX-12 and PX-866 presented at AACR Annual Meeting 3Data for Oncothyreon's PX-12 and PX-866 presented at AACR Annual Meeting 4Data for Oncothyreon's PX-12 and PX-866 presented at AACR Annual Meeting 5Anavex appoints Dr. Dimitri Goundis to lead Clinical Development 2Anavex appoints Dr. Dimitri Goundis to lead Clinical Development 3Anavex appoints Dr. Dimitri Goundis to lead Clinical Development 4
Collected from animals of U.S. origin.Collected from animals of U.S. origin. *Can be used in cell growth studies and immunoassays. *Sterility tested, * Mycoplasma tested upon request, *Endoto...
...
Mouse monoclonal [Ley 37D/G6] to LKB1 ( Abpromise for all tested applications). entrezGeneID: 6794 SwissProtID: Q15831...
... Solid phase reagent ... purification; Easy & unique ... Compatible with detergents and ... & recover non-envelop viruses ...
Biology Products: