Navigation Links
Penn Veterinary Medicine report new strategy to create genetically-modified animals
Date:9/20/2007

Researchers at the University of Pennsylvania School of Veterinary Medicine have demonstrated the potential of a new strategy for genetic modification of large animals. The method employs a harmless gene therapy virus that transfers a genetic modification to male reproductive cells, which is then passed naturally on to offspring.

Ina Dobrinski, associate professor and director of the Center for Animal Transgenesis and Germ Cell Research at Penn Vet, and her colleagues introduced adeno-associated virus, AAV, to male germline stem cells in both goats and mice. The study showed that AAV stably transduced male germ line stem cells and led to transgene transmission through the male germ line.

The findings, available online in The FASEB Journal and in the February 2008 print edition, are the first report of transgenesis via germ cell transplantation in a non-rodent species, a promising approach to germ line genetic modification. It also demonstrates that germline transduction and germ cell transplantation in large animals provides an approach that is potentially less costly than microinjection and cloning, the traditional methods used to generate transgenic large animal models for biomedical research.

Researchers used mouse germ cells harvested from experimentally induced cryptorchid donor testes that were then exposed in vitro to AAV vectors carrying a green fluorescent protein transgene and transplanted to germ cell-depleted recipient testes, resulting in colonization of the recipient testes by transgenic donor cells.

When researchers mated these recipient males with wild-type females, 10 percent of offspring carried the gene originally introduced into the transplanted germ cells, meaning the genetic modification had been passed on. To broaden the approach to non-rodent species, AAV-transduced germ cells from goats were transplanted to recipient males in which endogenous germ cells had been depleted by fractionated testicular
'/>"/>

Contact: Jordan Reese
jreese@pobox.upenn.edu
215-573-6604
University of Pennsylvania
Source:Eurekalert

Page: 1 2

Related biology news :

1. Researcher at UGA College of Veterinary Medicine identifies new way of combating viral diseases
2. Veterinary scientists explore poultry virus as cancer killer
3. Recent breakthroughs in common adult leukemia highlighted in New England Journal of Medicine
4. Leukemia Drug Breakthrough Study In New England Journal Of Medicine
5. Transport System Smuggles Medicines Into Brain
6. Papers of DNA Pioneer and Nobel Laureate Francis Crick Added to National Library of Medicine’s Profiles in Science Web Site
7. Affymetrix and the Karolinska Institutet Announce Translational Medicine Strategic Alliance
8. FDA Works To Speed The Advent Of New, More Effective Personalized Medicines
9. Genpathway and Baylor College of Medicine Identify New Genes in Breast Cancer
10. Computational Tool Predicts How Drugs Work In Cells, Advancing Efforts To Design Better Medicines
11. $6.5 Million Grant for Microarray Center at Yale School of Medicine
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/25/2014)... at the University of Houston, has written a ... development., "Introduction to Tissue Engineering: Applications and Challenges" ... field of artificial organ development. Metin Akay, founding ... served as a series editor on the book., ... books on the subject of growing artificial organs ...
(Date:7/25/2014)... research at the University of Adelaide has opened the way ... to powdery mildew. , In Australia, annual barley production is ... Powdery mildew is one of the most important diseases of ... have discovered the composition of special growths on the cell ... fungus into the leaf. , The research, by the ARC ...
(Date:7/25/2014)... (SB) is a complex congenital central nervous system ... of the neural tubes during the embryonic phase. ... and fecal incontinence and neurocognitive retardation. Such problems ... of life. Researchers at Ankara Physical Medicine and ... functional performance in children with SB, using the ...
Breaking Biology News(10 mins):University of Houston researcher publishes textbook on tissue engineering 2New hope for powdery mildew resistant barley 2
... FINDINGS: Short pieces of RNA, called microRNAs, control protein ... as messenger RNA or mRNA) to be disabled by ... Researchers have known that mammalian microRNAs control protein production ... wondered how much additional effects microRNAs impart by jamming ...
... OTO EXPO of the American Academy of Otolaryngology Head ... nose, and throat doctors in the world, will convene September ... 305 scientific research sessions, 594 posters, and several hundred instruction ... opportunity for journalists from around the world to cover breaking ...
... the cause of some of the most repugnant smells on ... forms actually has a sense of smell of its own. ... for the first time that bacteria have a molecular "nose" ... ammonia. Published today in Biotechnology Journal , their study ...
Cached Biology News:RNA snippets control protein production by disabling mRNAs 2RNA snippets control protein production by disabling mRNAs 3Bacteria breakthrough is heaven scent 2
(Date:7/25/2014)... At the request of the AMA, the ... for the public comment period (Docket No. FAA-2014-0396) for ... established by Congress as part of the FAA Modernization ... the new deadline for comments as September 23, 2014. ... published in the Federal Register on July 25, 2014, ...
(Date:7/24/2014)... New York, NY - July 24, 2014 -- Keryx ... results from the long-term, randomized, active control Phase 3 ... ferric iron-based phosphate binder, for the treatment of hyperphosphatemia ... The PERFECTED study (PhosphatE binding and iRon delivery with ... the Journal of the American Society of Nephrology ...
(Date:7/24/2014)... CA (PRWEB) July 24, 2014 ... the Global Supply Chain of the biotech industry. ... SCM Innovation Awards, brought to you by the ... global organization of supply chain management professionals for ... given to stakeholders of the Biotech industry – ...
(Date:7/24/2014)... PARK, Calif. , July 24, 2014 /PRNewswire/ ... company in the emerging field of regenerative medicine, ... webcast of a presentation to investors on Tuesday, ... p.m. PDT.  The presentation will include an overview ... To access the webcast, visit http://www.ustream.tv/channel/asterias-biotherapeutics ...
Breaking Biology Technology:FAA Grants Comment Extension at the Request of AMA 2Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 2Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 3Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 4Call for Submission: BSMA’s Supply Chain Management Innovation Awards 2Call for Submission: BSMA’s Supply Chain Management Innovation Awards 3Asterias Biotherapeutics Announces Live Investor Webcast 2Asterias Biotherapeutics Announces Live Investor Webcast 3Asterias Biotherapeutics Announces Live Investor Webcast 4
... leading,supplier of virtual reality based training applications within the ... of the Board and,Goran Malmberg as President and CEO ... and brings with him a vast,array of experience in ... of several Swiss private and charitable foundations. Mr.,Ruscoe is ...
... Ltd. (ASX: PXL),CEO Dr Jenny Harry has been ... in Washington DC at the Partnering for Global ... Foundation and organised by Bio,Ventures in Global Health, ... http://pgh.bio.org/opencms/PGH/2008/program/program.jsp , The Forum follows a report ...
... researchers at the National Institute of Standards and ... in solution as they dart around in three ... plans to patent, will lead to a better ... and, ultimately, process control techniques to optimize the ...
Cached Biology Technology:Mentice AB Announces Leadership Changes Within Its Worldwide Operations 2The Bill & Melinda Gates Foundation, BIO, and Bio Ventures for Global Health Invite Proteome Systems' CEO to Speak on TB Diagnostics Solutions At Partnering for Global Health Forum, Washington D.C. 2The Bill & Melinda Gates Foundation, BIO, and Bio Ventures for Global Health Invite Proteome Systems' CEO to Speak on TB Diagnostics Solutions At Partnering for Global Health Forum, Washington D.C. 3All done with mirrors: NIST microscope tracks nanoparticles in 3-D 2
... The ProteinChip Antibody Capture ... development and optimization steps ... antibody-antigen interactions. This kit ... differentiation of multiple antigens ...
...
... CLS number is a new product ... product number. If showing no availability ... Sigma-Aldrich number (R4276) or contact customer ... polystyrene (expanded surface, easy grip) ...
... Services offer a full range of peptide ... Services include: Peptide synthesis, crude product ... synthesis, purified product (70-95% pure) ... fluorescein, myristylation, phosphorylation, and rhodamine) ...
Biology Products: