Navigation Links
Pathogen study explores blocking effect of E. coli O157:H7 protein
Date:12/13/2013

MANHATTAN, KAN. -- Often the key to any victory is to fully understand your opponent. This is especially true when that opponent is a significant foodborne bacterial pathogen such as E. coli O157:H7.

Philip Hardwidge, associate professor at the College of Veterinary Medicine at Kansas State University, is studying how pathogens such as E. coli use proteins to block a host's innate immune system. This system is the body's first defense against infection, often presented in the body's mucosal surfaces such as those found in the intestine.

"In terms of infectious disease, this inhibition of the human innate immune response is absolutely critical for the bacteria's ability to cause an infection," said Hardwidge, who works in the diagnostic medicine and pathobiology department. "If we can identify choke points in the interaction between the bacterium and the host, we may be able to inhibit the bacterium and prevent its survival in an infected human being."

Hardwidge's lab received a multiyear grant from the National Institutes of Health to explore a protein expressed by pathogenic E. coli known as NleH1, which inhibits an important cellular signaling pathway called IKK/NF-B, or I-Kappa-Kinase/N-F-Kappa-B.

"This protein is one example of an injected bacterial protein that is able to block the innate immune system," Hardwidge said. "This protein has kind of an unusual mechanism that had not been seen in other bacterial or viral pathogens, so we're interested in understanding more about how this protein really works and whether it represents a good target for future therapeutics.

The exploration of these host-pathogen interactions requires the lab to use multidisciplinary approaches, including using animal models and advanced technologies such as quantitative polymerase chain reaction, or PCR.

"One of beauties of QPCR, or quantitative PCR, is that it gives a really reliable and easily to define comparative number of gene expression," said Mike Hays, microbiologist III in Hardwidge's lab. "It looks at a snapshot in time in that cellular environment and it could tell us at that snapshot in time, in that window, what the expression levels are of the genes that we're interested in."

Understanding how these bacterial proteins function in the host-pathogen interaction may also have applications for other human diseases.

"For example, many autoimmune diseases, many cancers and even diabetes are caused in part by an overactive component of this innate immune system," Hardwidge said. "Using information from bacteria and viruses that have evolved to block this overactive immune response, we may be able to engineer some of these bacteria proteins as potential therapeutics."

Through collaborations at Kansas State University and his position as a Chinese Academy of Sciences' senior international scientist, Hardwidge's future research will also explore both the role that the microbes that naturally live in the human body have in host-pathogen interactions and other forms of E. coli that afflict humans. Armed with this knowledge, researchers at the College of Veterinary Medicine will be able to reveal new strategies for defeating pathogens such as E. coli O157:H7.


'/>"/>

Contact: Philip Hardwidge
hardwidg@vet.k-state.edu
785-532-2506
Kansas State University
Source:Eurekalert  

Related biology news :

1. New technique identifies pathogens in patient samples faster, in great detail
2. Study to identify functions of hypothetical genes in 2 infectious disease pathogens
3. Tuberculosis genomes portray secrets of pathogens success
4. Helper cells aptly named in battle with invading pathogens
5. Resistance gene found against Ug99 wheat stem rust pathogen
6. Recent progress in gene-sensing strategies for rapid detection of foodborne pathogens
7. Discovery of how a gene that regulates factors involved in bacteria pathogenicity acts
8. University of Toronto breakthrough allows fast, reliable pathogen identification
9. Pathogen turns protein into a virulence factor in 1 easy step
10. Predicting the next eye pathogen; analysis of a novel adenovirus
11. Vaccine adjuvant uses host DNA to boost pathogen recognition
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Pathogen study explores blocking effect of E. coli O157:H7 protein
(Date:11/15/2016)... Research and Markets has announced the addition of the ... offering. ... The global bioinformatics market is ... Billion in 2016, growing at a CAGR of 21.1% during the ... driven by the growing demand for nucleic acid and protein sequencing, ...
(Date:6/22/2016)... ANGELES , June 22, 2016 /PRNewswire/ ... identity management and verification solutions, has partnered ... edge software solutions for Visitor Management, Self-Service ... provides products that add functional enhancements ... partnership provides corporations and venues with an ...
(Date:6/15/2016)... ALBANY, New York , June 15, 2016 ... published a new market report titled "Gesture Recognition Market ... Trends and Forecast, 2016 - 2024". According to the ... at USD 11.60 billion in 2015 and is ... and reach USD 48.56 billion by 2024.  ...
Breaking Biology News(10 mins):
(Date:12/5/2016)... 5, 2016  Renova™ Therapeutics, a biotechnology company ... and other chronic diseases, announced that Catherine ... Chief Financial Officer (CFO), effective today. ... of experience in financial management for a variety ... companies. Most recently, Ms. Bovenizer was the Vice ...
(Date:12/5/2016)... -- Research and Markets has announced the addition ... Markets and Companies" to their offering. ... , , ... genome variations, development of sequencing technologies, and their applications. Current ... developing them. Various applications of sequencing are described including those ...
(Date:12/4/2016)... , Dec. 3, 2016  In five studies ... Hematology (ASH) Annual Meeting and Exposition in ... engineering methods to improve the delivery of life-saving treatments ... new methods are designed to carry therapies directly to ... most, which could provide a substantial advantage over traditional, ...
(Date:12/2/2016)... NORWALK, Conn. , Dec. 2, 2016  The ... research presentations from the MMRF CoMMpass Study SM —the ... and accelerating precision medicine in multiple myeloma—will be presented ... (ASH) Annual Meeting & Exposition in San ... are helping to optimize treatment strategies, as well as ...
Breaking Biology Technology: