Recent years have seen significant outbreaks of listeriosis on both sides of the Atlantic. Although the disease can usually be treated successfully, it is occasionally fatal, most frequently in pregnant women or immunocompromized people. And even when treatment is effective, the symptoms are anything but pleasant and include fever and muscle aches along with diarrhoea and other gastrointestinal symptoms. The old adage is clearly true: prevention is better than cure.
Prevention of listeriosis relies on killing the causative agent, normally the bacterium Listeria monocytogenes, in dairies and other food-processing facilities. A number of disinfectants are used for this purpose, most often quaternary ammonium compounds such as benzalkonium chloride (BC). Unfortunately, however, many strains of listeria seem to be developing resistance to these agents, although the underlying mechanisms have remained obscure. Together with colleagues in Ireland, the group of Stephan Schmitz-Esser of the Vetmeduni's Institute for Milk Hygiene has provided convincing evidence that a novel piece of DNA in the bacteria is involved.
The scientists used next-generation sequencing techniques to determine the DNA sequences of two strains of listeria known to be resistant to BC. When they examined the sequences they noticed a region of DNA of ca. 5 kb that was strikingly different in composition from the remainder of the genome. The bacteria seem to have acquired this novel element fairly recently and Schmitz-Esser termed it Tn6188 (so-called transposons are frequent in bacterial genomes, explaining the high number).
Of course, the presence of Tn6188 in two strains resistant to BC might merely be a coincidence. The researchers thus screened an additional 90 strains of listeria for the element, finding it in ten of them. The ten strains harbouring Tn6188 turned out to be far less sensitive to benzalkonium chloride. One of the five proteins that could be
|Contact: Susanna Kautschitsch|
University of Veterinary Medicine -- Vienna