The construction of the maize optical map represents the first physical map of a eukaryotic genome larger than 400 Mb that was created de novo from individual genomic DNA molecules. "The maize optical map is by far the most complex example of genome analysis via single molecules," says Dr. David Schwartz of the University of Wisconsin-Madison. "It was created using completely de novo techniques which greatly surpass conventional sequencing and all available next-generation sequencing methods and platforms in terms of completeness, speed, accuracy and cost. This work points the way for new platforms dealing with personal genomics."
Maize Inbreds Exhibit High Levels of Copy Number Variation (CNV) and Presence/Absence Variation (PAV) in Genome Content
Springer NM, Ying K, Fu Y, Ji T, Yeh C-T, et al.
There is a growing appreciation for the role of genome structural variation in creating phenotypic variation within a species. Springer et al. used comparative genomic hybridization to compare the genome structures of two maize inbred lines, B73 and Mo17, and observed that whole genes are missing in one inbred relative to the other. The data reinforce the view that maize is highly polymorphic (assuming different forms) but also show that there are often large genomic regions that have little or no variation.
Sequencing, Mapping, and Analysis of 27,455 Maize Full-Length cDNAs
Soderlund C, Descour A, Kudrna D, Bomhoff M, Boyd L, et al.
To complement the completion of sequencing the maize B73 genome, Yu, Soderlund and Walbot sequenced 27,455 full-length cDNAs from two maize B73 libraries, representing the gene transcripts from most tissues and common abiotic stress conditions. They discovered about 1,600 unique maize genes, not found in other plant databases, that they anticipate will allow a better understanding o
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