Navigation Links
P[acman]-generated fruit fly gene 'library': A new research tool
Date:5/24/2009

HOUSTON -- (May 24, 2009) -- Using a specially adapted tool called P[acman], a collaboration of researchers led by Baylor College of Medicine has established a library of clones that cover most of the genome of Drosophila melanogaster (fruit fly) and should speed the pace of genetic research.

In a report in the current online issue of the journal Nature Methods, Dr. Hugo Bellen (http://flypush.imgen.bcm.tmc.edu/lab/), a professor of molecular and human genetics at BCM and a Howard Hughes Medical Institute investigator, and his colleagues describe the new libraries.

P[acman] developed by Dr. Koen Venken (http://flypush.imgen.bcm.tmc.edu/lab/koenv/index.html) in Bellen's laboratory allows scientists to study large chunks of DNA in living flies. The vector officially P/phiC31 artificial chromosome for manipulation combines different technologies: a specially designed bacterial artificial chromosome (BAC) that allows maintenance of large pieces of DNA in bacteria, recombineering that allows the manipulation of large pieces of DNA in bacteria, and the ability to insert the genomic DNA into the genome of the fly at a specific site using phiC31-mediated transgenesis.

Venken adapted the P[acman] vector to create genomic libraries, so that a researcher can choose a gene and find the corresponding clones in the library that cover that gene. Their collaborators at Lawrence Berkeley National Laboratory, Drs. Roger Hoskins and Joseph Carlson, played a key role in the design, construction, and annotation of the libraries.

"You can insert a single copy of a gene and rescue a mutation, or do a structure/function analysis of the gene," Bellen said. "If you don't know where the gene is expressed, you can tag it, put it back and locate where it is expressed."

The library is available at

Contact: Glenna Picton
picton@bcm.edu
713-798-4710
Baylor College of Medicine
Source:Eurekalert

Page: 1 2

Related biology news :

1. Drinking 100 percent fruit juice is associated with lower risk of obesity and metabolic syndrome
2. Caltech researchers train computers to analyze fruit-fly behavior
3. Fruit flies sick from mating
4. Tuning in on cellular communication in the fruit fly
5. Scientists unlock possible aging secret in genetically altered fruit fly
6. When it comes to sleep research, fruit flies and people make unlikely bedfellows
7. Women double fruit, veggie intake with switch to Mediterranean diet plan
8. How eating fruit and vegetables can improve cancer patients response to chemotherapy
9. Substance found in fruits and vegetables reduces likelihood of the flu
10. Biochemists manipulate fruit flavor enzymes
11. Parents shape whether their children learn to eat fruits and vegetables
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/23/2015)... , Feb. 23, 2015 NXT-ID, Inc. (NASDAQ: ... "Company"), a biometric authentication company focused on the growing ... provisional patent 62114357 for DISTRIBUTED VOICE DIRECTED ... NXT-ID introduces a new groundbreaking payment method. Payment accounts ... account may only be accessed if both the speech ...
(Date:2/13/2015)... Feb. 13, 2015 ACT Genomics Co., ... aims to transform cancer genomic information into precision ... company has raised US$ 8 million in the its ... Taipei, Taiwan , ACT Genomics ... With the aim to implement next generation sequencing ...
(Date:2/11/2015)... DALLAS , February 11, 2015 /PRNewswire/ ... market research report "Access Control Market by Product ... (Commercial, Residential, Military and Defense, Government, Industrial, Healthcare, ... , published by MarketsandMarkets, the Access Control ... by 2020, growing at a CAGR of ...
Breaking Biology News(10 mins):NXT-ID Patents Voice Directed Payments 2NXT-ID Patents Voice Directed Payments 3ACT Genomics Raises $8 Million in its First Private Funding Round 2Access Control Market Worth $10.4 Billion by 2020 2Access Control Market Worth $10.4 Billion by 2020 3Access Control Market Worth $10.4 Billion by 2020 4
... to decisions about land use than climate change, population ... Mountains of Morocco, individual religious beliefs in local Saints ... use their environment and manage local resources. These findings1 ... Barcelona in Spain, and his colleagues, published online in ...
... call it comparing apples and oranges, but lining up ... Scientists can see how species have evolved, pinpoint which ... probably are crucial to the animals, survival, or sketch ... is spotty. , But the tools used to align ...
... Ore. Researchers at Oregon State University have made an ... toward robots that not only can walk and run effectively, ... an optimal approach with robotic mechanisms, studies are moving closer ... military, create prosthetic limbs for humans that work much better, ...
Cached Biology News:Farmers' beliefs on a higher plain 2Genome comparison tools found to be susceptible to slip-ups 2Advances made in walking, running robots 2Advances made in walking, running robots 3
(Date:2/27/2015)... The fully automated supercritical fluidic chromatography-based ... analyze up to 48 samples utilizing automatic extraction ... targets by mass spectrometry. , The Nexera ... requirements of a wide range of applications, including ... search for disease biomarkers, additives in forming polymers, ...
(Date:2/27/2015)... CA (PRWEB) February 27, 2015 Cytokinetics, ... forces on February 28 with patients and health care ... awareness for Rare Disease Day®. Rare Disease Day ... and calling attention to the special challenges faced by ... them. , “Cytokinetics is proud to stand alongside patients ...
(Date:2/26/2015)... 2015 On behalf of biotech client ... Wilentz, Goldman & Spitzer, P.A. (Wilentz) secured a significant ... Inc. in excess of $10 million. The jury determined ... and that it improperly hired one or more of ... for judgment notwithstanding the verdict, a new trial, and ...
(Date:2/26/2015)... 2015   Regulus Therapeutics Inc . (NASDAQ: ... and development of innovative medicines targeting microRNAs, today announced ... Chief Executive Officer of Regulus, will present a company ... Annual Healthcare Conference on Wednesday, March 4, 2015 at ... the Boston Marriott Copley Place.  The ...
Breaking Biology Technology:Shimadzu Corporation Launches Nexera UC - The World’s Foremost Unified Chromatography System 2Shimadzu Corporation Launches Nexera UC - The World’s Foremost Unified Chromatography System 3Cytokinetics Joins Global Movement to Raise Awareness for Rare Diseases 2Cytokinetics Joins Global Movement to Raise Awareness for Rare Diseases 3Cytokinetics Joins Global Movement to Raise Awareness for Rare Diseases 4Cytokinetics Joins Global Movement to Raise Awareness for Rare Diseases 5Cytokinetics Joins Global Movement to Raise Awareness for Rare Diseases 6Wilentz Client GenScript Awarded $10 Million in Trade Secrets Case Against Genewiz 2Wilentz Client GenScript Awarded $10 Million in Trade Secrets Case Against Genewiz 3Regulus to Present at Cowen and Company 35th Annual Healthcare Conference 2Regulus to Present at Cowen and Company 35th Annual Healthcare Conference 3
... ... has recently gained popularity for treatment of fungal nails. OnychoLase™ Laser Nail ... “prescreening” practioners and making sure that they satisfy the requirements to bear the brand ... , ...
... Silence Therapeutics plc (AIM: SLN) ("Silence" ... States patent 7,723,316, titled Compositions and Methods of ... Disease, by the United States Patent and Trademark Office ... portfolio of RNA interference (RNAi) intellectual property, is broadly ...
... 2010 Hailed as a "cross between a high-speed ... have developed a new, practical technology for fabricating tiny ... Badrossamay to the fairground treat of spun sugar is ... and pushes out 100 nanometer-diameter polymer-based threads using a ...
Cached Biology Technology:OnychoLase™ Laser Nail Center Provides New Laser Nail Therapy to Help Fight Against Fungal Nails 2OnychoLase™ Laser Nail Center Provides New Laser Nail Therapy to Help Fight Against Fungal Nails 3Silence Therapeutics Issued Novel RNAi Patent Covering High-Value Cancer Target in United States 2Silence Therapeutics Issued Novel RNAi Patent Covering High-Value Cancer Target in United States 3Silence Therapeutics Issued Novel RNAi Patent Covering High-Value Cancer Target in United States 4Inspired by a cotton candy machine, engineers put a new spin on creating tiny nanofibers 2Inspired by a cotton candy machine, engineers put a new spin on creating tiny nanofibers 3
Porcine Aortic Endothelial Cells (PAOEC) (>500,000 cells)...
Bovine Endometrium Epithelial Cells (BEnEpC) (>500,000 cells)...
PC based system for advanced chemifluorescent, chemiluminescent, fluorescent and colorimetric, microarray and microscope imaging and analysis....
Pendrin (H-195)...
Biology Products: