Navigation Links
Open science journal publishes attempt to reproduce high-profile stem cell acid bath study
Date:5/8/2014

In a study published today in F1000Research, Professor Kenneth Lee of the Chinese University of Hong Kong reveals the full experimental results of an attempt to replicate a controversial study published in Nature recently that suggested that bathing somatic cells in acid can reprogram them to induced pluripotent stem cells (iPS cells). With systematically collected and fully available data, Lee and his colleagues report that carefully replicating the original acid-treatment method does not induce pluripotency in two types of mouse somatic cells, including those used in the original study.

iPS cells have the unique ability to give rise to most other cell types, and have potentially wide therapeutic applications. This characteristic can be triggered in other cells by transforming them into iPS cells, which further increases the potential for stem cell therapies. Earlier this year, a group from Japan reported a greatly simplified procedure for producing iPS cells by bathing somatic cells in an acid bath.

To verify these claims, Kenneth Lee set out to replicate the work in his own lab, and documented his experiments online in the process, leading to lively online discussions between stem cell scientists who provided feedback and commentary along the way. Using both white blood cells isolated from the spleen of neonatal mice - the same cells used in the original study - and lung fibroblasts, Lee was unable to replicate the original findings, and has now published the full results of his study in F1000Research.

Unlike most journals, all research papers in the open access journal F1000Research are accompanied by the full underlying datasets so other researchers will be able to re-analyse and more easily reproduce the work. In addition, F1000Research employs open peer review by invited expert scientists, which occurs after publication and is published in full online alongside the paper, removing much of the potential bias that often occurs in traditional anonymous pre-publication peer review.

"Publishing Professor Lees attempted replication study in this unique and open manner will facilitate a less-hyped and more informed debate and demonstrates the value of this extra level of transparency in the publication process", says Daniel Marovitz, CEO of F1000.

Lee's study will now undergo this transparent invited peer review process and anyone interested to see referees' views as they come in can follow the paper by clicking 'Track' on the published article.


'/>"/>

Contact: Eleanor Howell
press@f1000.com
44-020-763-19129
Faculty of 1000
Source:Eurekalert

Related biology news :

1. National Academy of Sciences elects Cary Institute of Ecosystem Studies scientist
2. National Academy of Sciences elects 5 from UChicago, Marine Biological Laboratory
3. Study in Science finds missing piece of biogeochemical puzzle in aquifers
4. Jerry Melillo of MBL Ecosystems Center elected to National Academy of Sciences
5. Arizona State University professor is elected to the National Academy of Sciences
6. BRIC carries big science in small canisters
7. Two San Diego scientists elected to the National Academy of Sciences
8. New Translational Addiction Sciences Center poised to make headway in treatment of addiction
9. ESA to publish the Journal of Insect Science
10. Bioethicists use theatrical narratives to bridge the gap between society and science
11. PETA science consortium to present hazard testing strategy at nanotoxicology meeting
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/30/2017)... 2017   Invitae Corporation (NYSE: ... companies, today announced that it will report its fourth ... guidance on Monday, February 13, 2017, and Invitae,s management ... 4:45 p.m. Eastern / 1:45 p.m. Pacific. ... review financial results, guidance, and recent developments and will ...
(Date:1/25/2017)... The Elements of Enterprise Information Security ... of a comprehensive set of business processes and ... identities and providing a secured and documented access ... number of programs opted by enterprises to maintain ... processes and changing policies. However, there are some ...
(Date:1/23/2017)... , Jan. 23, 2017  The latest mobile ... smartphone prices have dropped dramatically. The quarterly average price ... 2013 to $276 in Q4 2016.  There are now ... price of $116, up from just 28 a year ... According to Maxine Most , Acuity Market ...
Breaking Biology News(10 mins):
(Date:2/21/2017)... Scientists From Two Companies to Accelerate Development ... ... STEER, creator of advanced materials platform technologies that effectively ... pharmaceuticals, food and nutraceuticals, biomaterials and biorefining, today announced a ... company, on creating co-rotating twin screw extruder technology to ...
(Date:2/20/2017)...  This Report analyzes the worldwide markets for Bioinformatics in US$ ... separate comprehensive analytics for the US, Canada , ... Asia-Pacific , Latin America , and ... http://www.reportlinker.com/p04707099-summary/view-report.html ... Annual estimates and forecasts are provided for the period 2015 through ...
(Date:2/20/2017)... , ... February 20, 2017 ... ... therapeutic success among radiotherapy patients, prevent chest wall collapses in pre-term infants ... alignments will receive a total of $600,000 in funding through the ninth ...
(Date:2/19/2017)... ... February 19, 2017 , ... Expanding ... ventured outside of weighing equipment with the goal of expanding the reach of ... the line of Starter water analysis meters were introduced into the market in ...
Breaking Biology Technology: