Navigation Links
Open science journal publishes attempt to reproduce high-profile stem cell acid bath study
Date:5/8/2014

In a study published today in F1000Research, Professor Kenneth Lee of the Chinese University of Hong Kong reveals the full experimental results of an attempt to replicate a controversial study published in Nature recently that suggested that bathing somatic cells in acid can reprogram them to induced pluripotent stem cells (iPS cells). With systematically collected and fully available data, Lee and his colleagues report that carefully replicating the original acid-treatment method does not induce pluripotency in two types of mouse somatic cells, including those used in the original study.

iPS cells have the unique ability to give rise to most other cell types, and have potentially wide therapeutic applications. This characteristic can be triggered in other cells by transforming them into iPS cells, which further increases the potential for stem cell therapies. Earlier this year, a group from Japan reported a greatly simplified procedure for producing iPS cells by bathing somatic cells in an acid bath.

To verify these claims, Kenneth Lee set out to replicate the work in his own lab, and documented his experiments online in the process, leading to lively online discussions between stem cell scientists who provided feedback and commentary along the way. Using both white blood cells isolated from the spleen of neonatal mice - the same cells used in the original study - and lung fibroblasts, Lee was unable to replicate the original findings, and has now published the full results of his study in F1000Research.

Unlike most journals, all research papers in the open access journal F1000Research are accompanied by the full underlying datasets so other researchers will be able to re-analyse and more easily reproduce the work. In addition, F1000Research employs open peer review by invited expert scientists, which occurs after publication and is published in full online alongside the paper, removing much of the potential bias that often occurs in traditional anonymous pre-publication peer review.

"Publishing Professor Lees attempted replication study in this unique and open manner will facilitate a less-hyped and more informed debate and demonstrates the value of this extra level of transparency in the publication process", says Daniel Marovitz, CEO of F1000.

Lee's study will now undergo this transparent invited peer review process and anyone interested to see referees' views as they come in can follow the paper by clicking 'Track' on the published article.


'/>"/>

Contact: Eleanor Howell
press@f1000.com
44-020-763-19129
Faculty of 1000
Source:Eurekalert

Related biology news :

1. National Academy of Sciences elects Cary Institute of Ecosystem Studies scientist
2. National Academy of Sciences elects 5 from UChicago, Marine Biological Laboratory
3. Study in Science finds missing piece of biogeochemical puzzle in aquifers
4. Jerry Melillo of MBL Ecosystems Center elected to National Academy of Sciences
5. Arizona State University professor is elected to the National Academy of Sciences
6. BRIC carries big science in small canisters
7. Two San Diego scientists elected to the National Academy of Sciences
8. New Translational Addiction Sciences Center poised to make headway in treatment of addiction
9. ESA to publish the Journal of Insect Science
10. Bioethicists use theatrical narratives to bridge the gap between society and science
11. PETA science consortium to present hazard testing strategy at nanotoxicology meeting
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/2/2016)... June 2, 2016 The Department of ... awarded the 44 million US Dollar project, for the ... Plates including Personalization, Enrolment, and IT Infrastructure , ... the production and implementation of Identity Management Solutions. Numerous renowned ... Decatur was selected for the most ...
(Date:6/1/2016)... NEW YORK , June 1, 2016 ... Biometric Technology in Election Administration and Criminal Identification to ... According to a recently released TechSci Research report, " ... Sector, By Region, Competition Forecast and Opportunities, 2011 - ... $ 24.8 billion by 2021, on account of growing ...
(Date:5/16/2016)... NEW YORK , May 16, 2016   ... authentication solutions, today announced the opening of an IoT ... to strengthen and expand the development of embedded ... provides an unprecedented level of convenience and security with ... to authenticate one,s identity aside from DNA. EyeLock,s platform ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... ... June 24, 2016 , ... While the majority of commercial spectrophotometers and ... and the 6000i models are higher end machines that use the more unconventional z-dimension ... light beam from the bottom of the cuvette holder. , FireflySci has developed ...
(Date:6/23/2016)... , ... June 23, 2016 , ... ... release of its second eBook, “Clinical Trials Patient Recruitment and Retention Tips.” Partnering ... retention in this eBook by providing practical tips, tools, and strategies for clinical ...
(Date:6/23/2016)... 23, 2016   Boston Biomedical , an ... designed to target cancer stemness pathways, announced that ... Orphan Drug Designation from the U.S. Food and ... cancer, including gastroesophageal junction (GEJ) cancer. Napabucasin is ... inhibit cancer stemness pathways by targeting STAT3, and ...
(Date:6/23/2016)... -- A person commits a crime, and the detective uses ... criminal down. An outbreak of foodborne illness makes ... uses DNA evidence to track down the bacteria that caused ... not. The FDA has increasingly used a complex, cutting-edge technology ... Put as simply as possible, whole genome sequencing is a ...
Breaking Biology Technology: