Navigation Links
October 2008 Biology of Reproduction highlights
Date:9/18/2008

The role of the Dicer. MicroRNAs are emerging as major transcriptional and post-transcriptional regulators of gene expression. DICER, an RNaseIII enzyme, plays a role in the biogenesis of microRNAs; recently there has been a flurry of activity to determine the developmental role of DICER by whole-organism and conditional gene disruptions. In a paper on p. 696 of this issue, Maatouk et al. add important new information to the emerging story by demonstrating that DICER1 is required for normal spermatogenesis in the mouse. These authors used a conditional knockout (CKO) approach to delete Dicer1 in the germline. Because the CKO mice exhibited transient fertility, an elegant reporter strategy was used to demonstrate that elongated spermatids present in the CKO testes did express the Cre recombinase; therefore, it is likely that differentiation to this stage can occur in the absence of DICER1. Nonetheless, Dicer1 CKO spermatogenic cells exhibited disruption of microRNA processing and both quantitative and qualitative defects leading to the formation of functionally abnormal sperm. These data, together with other recent publications, suggest complex roles for microRNAs and their processing enzymes in both male and female germ cells and promise much interesting work to come.

Danielle M. Maatouk, Kate L. Loveland, Michael T. McManus, Karen Moore, and Brian D. Harfe. Dicer1 Is Required for Differentiation of the Mouse Male Germline. Biol Reprod 2008; 79:696-703. Published online in BOR-Papers In Press 16 July 2008; DOI 10.1095/biolreprod.108.067827


Perils of culture. There is growing interest in the developmental origins of adult disease, and effects of both nutrition and environment on oocytes and embryos on adult health of offspring have been demonstrated. However, mechanisms by which deleterious effects are established during these early developmental stages are unknown, although epigenetic processes have been suspected. Morgan et al. elegantly bolster the epigenetic hypothesis in a paper on p. 618 of this issue. The authors used a mouse "reporter" model for changes in methylation: the IAP (intracisternal A-particle) element that controls expression at the coat-color Avy (agouti viable yellow) allele; mice bearing this allele exhibit coat-color differences reflective of the extent of methylation on the IAP element. Morgan et al. found highly significant differences in color coats among offspring derived from either in vivo or in vitro preimplantation development, demonstrating that conditions for preimplantation development affected epigenetic regulation of the Avy locus. Thus, culture conditions used in this study did not mimic the normal (in vivo) environment for preimplantation development and this difference resulted in a clear effect on the adult phenotype. The Avy model will facilitate optimization of preimplantation culture of mouse embryos, and lessons learned from this approach in turn should be valuable for optimizing human preimplantation culture environments.

Hugh D. Morgan, Xing L. Jin, Aiqing Li, Emma Whitelaw, and Chris O'Neill. The Culture of Zygotes to the Blastocyst Stage Changes the Postnatal Expression of an Epigentically Labile Allele, Agouti Viable Yellow, in Mice. Biol Reprod 2008; 79: 618-623. Published online in BOR-Papers In Press 18 June 2008; DOI 10.1095/biolreprod.108.068213


'/>"/>

Contact: Judith Jansen
ssradmin@ssr.org
608-256-2777
Society for the Study of Reproduction
Source:Eurekalert

Related biology news :

1. October Geology and GSA Today media highlights
2. Story tips from the Department of Energys Oak Ridge National Laboratory, October 2007
3. Tips from the Journals of the American Society for Microbiology
4. Society for General Microbiology 161st Meeting, University of Edinburgh
5. Systems Biology poised to revolutionize the understanding of cell function and disease
6. Europe needs collective effort on System Biology, says ESF Task Force
7. American Society for Microbiology honors Lucas R. Hoffman
8. American Society for Microbiology honors Joanna Kubler-Kielb
9. American Society for Microbiology honors Hung Ton-That
10. American Society for Microbiology honors Thomas E. Wellems
11. American Society for Microbiology honors Jason T. Blackard
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/24/2016)... care by providing unparalleled technology to leaders of the medical imaging industry.  As such, ... to the range of products distributed by Ampronix. Photo - http://photos.prnewswire.com/prnh/20160524/371420 ... ... ... With ...
(Date:5/3/2016)... May 3, 2016  Neurotechnology, a provider of ... MegaMatcher Automated Biometric Identification System (ABIS) , a ... projects. MegaMatcher ABIS can process multiple complex biometric ... combination of fingerprint, face or iris biometrics. It ... and MegaMatcher Accelerator , which have ...
(Date:4/19/2016)... DUBAI , UAE, April 20, 2016 ... can be implemented as a compact web-based "all-in-one" system ... in the biometric fingerprint reader or the door interface ... requirements of modern access control systems. The minimal dimensions ... the ID readers into the building installations offer considerable ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... , June 27, 2016  Global demand for ... percent through 2020 to $7.2 billion.  This market ... beverages, cleaning products, biofuel production, animal feed, and ... diagnostics, and biocatalysts). Food and beverages will remain ... by increasing consumption of products containing enzymes in ...
(Date:6/27/2016)... , ... June 27, 2016 , ... ... bring innovative medical technologies, services and solutions to the healthcare market. The company's ... of various distribution, manufacturing, sales and marketing strategies that are necessary to help ...
(Date:6/24/2016)... June 24, 2016 Epic Sciences unveiled ... cancers susceptible to PARP inhibitors by targeting homologous ... (CTCs). The new test has already been incorporated ... multiple cancer types. Over 230 clinical ... response pathways, including PARP, ATM, ATR, DNA-PK and ...
(Date:6/23/2016)... CAMBRIDGE, Mass. , June 23, 2016 /PRNewswire/ ... the development of novel compounds designed to target ... compound, napabucasin, has been granted Orphan Drug Designation ... in the treatment of gastric cancer, including gastroesophageal ... cancer stemness inhibitor designed to inhibit cancer stemness ...
Breaking Biology Technology: