Navigation Links
ORNL protein analysis investigates marine worm community
Date:5/9/2012

Techniques used by researchers from the Department of Energy's Oak Ridge National Laboratory to analyze a simple marine worm and its resident bacteria could accelerate efforts to understand more complex microbial communities such as those found in humans.

In a paper published in the Proceedings of the National Academy of Sciences, a multi-institutional research team analyzed the proteins found in a marine worm known as Olavius algarvensis. The worm lacks a digestive system and relies on microbes that live in its body to process its waste and provide energy. Previous research, however, had not untangled the metabolic details of this mutually beneficial, or symbiotic, relationship.

"This community is like the simplest form of the human gut," said coauthor Nathan VerBerkmoes of ORNL. "It is a model system to understand symbiosis."

While some complex microbial communities such as the human gastrointestinal system contain hundreds of thousands of microbes, the marine worm relies on only four to five bacteria. Understanding the simple network of relationships between the worm and its symbionts, however, still required novel techniques to analyze the functions of a whole system rather than individual parts.

"Very often, you'll have a community that does function x," VerBerkmoes said. "But if you try to isolate any one of those microbes out of the community and get it to do that function, one microbe alone can't do it. Furthermore these microbes cannot be easily isolated and studied by traditional molecular approaches. "

To unravel the interactions of the worm community, an ORNL team led by VerBerkmoes used metaproteomics, a form of analysis that identifies and categorizes the proteins in an organism's cell. Genomics, which yields the DNA sequence of an organism, can predict a cell's behavior, whereas proteomics gives scientists a real-time snapshot of what actually happens in the cell's metabolism.

The combination of genomics and proteomics can also help explain apparent redundancies in a system where several organisms appear at first glance to perform the same function.

"One of the key questions in metagenomic analyses of complex symbiotic consortia, including those of the human gut, is why there is so much functional redundancy," said lead author Nicole Dubilier of the Max Planck Institute for Marine Microbiology. "Our metaproteomic analyses of the bacteria found in O. algarvensis indicate functional differences in the metabolism of two symbionts despite their genetic similarities. This appears to be a common theme in microbial communities."

Metaproteomics provided indirect evidence for the research team's hypotheses about the worm community's metabolism, such as the potential use of hydrogen and carbon monoxide as energy sources. VerBerkmoes adds that while their analysis rapidly provided a broad overview of the system, the techniques do not confirm the specifics of individual protein behavior or function, which must be established via further direct biochemical studies.

The research is published as "Metaproteomics of a gutless marine worm and its symbiotic microbial community reveal unusual pathways for carbon and energy use." Coauthors include ORNL's Jacque Young, Yun-Juan Chang and Manesh Shah, and researchers from the Max Planck Institute for Marine Microbiology, the University of Greifswald, the University of Freiburg, the Institute of Marine Biotechnology in Greifswald, and the HYDRA Institute for Marine Sciences. The project was lead by Manuel Kleiner and Nicole Dubilier from the Max Planck Institute for Marine Microbiology.


'/>"/>

Contact: Morgan McCorkle
mccorkleml@ornl.gov
865-574-7308
DOE/Oak Ridge National Laboratory
Source:Eurekalert

Related biology news :

1. Discovery of a new family of key mitochondrial proteins for the function and viability of the brain
2. Protein signal is crucial for accurate control of insect size
3. Key proteins newly discovered form and function may provide novel cancer treatment target
4. In protein folding, internal friction may play a more significant role than previously thought
5. High levels of TRAIL protein in breast milk might contribute to anticancer activity
6. New study finds a protein combination is best to consume post-workout for building muscle
7. HUNT Biosciences and SomaLogic collaborate to validate protein biomarkers of cardiovascular risk
8. Researchers identified a protein useful in predicting the risk of pulmonary metastases in breast cancer patients
9. Newly found protein helps cells build tissues
10. Plant research reveals new role for gene silencing protein
11. Protein jailbreak helps breast cancer cells live
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/14/2016)... 2016 BioCatch ™, the ... announced the appointment of Eyal Goldwerger as ... Goldwerger,s leadership appointment comes at a time of ... deployment of its platform at several of the world,s ... discerns unique cognitive and physiological factors, is a winner ...
(Date:3/23/2016)... , March 23, 2016 ... erhöhter Sicherheit Gesichts- und Stimmerkennung mit Passwörtern ... (NASDAQ: MESG ), ein führender ... das Unternehmen mit SpeechPro zusammenarbeitet, um erstmals ... Finanzdienstleistungsbranche, wird die Möglichkeit angeboten, im Rahmen ...
(Date:3/17/2016)... ABI Research, the leader in transformative ... market will reach more than $30 billion by ... Consumer electronics, particularly smartphones, continue to boost the ... reach two billion shipments by 2021 at a ... Research Analyst at ABI Research. "Surveillance is also ...
Breaking Biology News(10 mins):
(Date:4/28/2016)... MA (PRWEB) , ... April 28, 2016 , ... ... Asymmetrex will deliver a talk on its first-in-class technologies for tissue ... 2016 Meeting on RNAiMicroRNA Biology to Reprogramming & CRISPR-based Genome Engineering ...
(Date:4/27/2016)... ... ... Cambridge Semantics, the leading provider of Smart Data analytic and data management ... The Silicon Review’s “20 Fastest Growing Big Data Companies of 2016.” , “From ... end users facing some of the most complex data challenges in the industry,” said ...
(Date:4/27/2016)... , ... April 27, 2016 , ... ... Touch screen mobile devices with fingerprint recognition for secure access, voice recognition for ... a few ways consumers are interacting with biometrics technology today. But if ...
(Date:4/27/2016)... ... 27, 2016 , ... The Board of Directors of Biohaven ... Tilton as Chief Commercial Officer.  Mr. Tilton joined Biohaven from Alexion Pharmaceuticals, Inc. ... responsible for the commercialization of multiple orphan drug indications. Mr. Tilton has ...
Breaking Biology Technology: