Navigation Links
ORNL process improves catalytic rate of enzymes by 3,000 percent
Date:4/17/2012

Light of specific wavelengths can be used to boost an enzyme's function by as much as 30 fold, potentially establishing a path to less expensive biofuels, detergents and a host of other products.

In a paper published in The Journal of Physical Chemistry Letters, a team led by Pratul Agarwal of the Department of Energy's Oak Ridge National Laboratory described a process that aims to improve upon nature and it happens in the blink of an eye.

"When light enters the eye and hits the pigment known as rhodopsin, it causes a complex chemical reaction to occur, including a conformational change," Agarwal said. "This change is detected by the associated protein and through a rather involved chain of reactions is converted into an electrical signal for the brain."

With this as a model, Agarwal's team theorized that it should be possible to improve the catalytic efficiency of enzyme reactions by attaching chemical elements on the surface of an enzyme and manipulating them with the use of tuned light.

The researchers introduced a light-activated molecular switch across two regions of the enzyme Candida antarctica lipase B, or CALB which breaks down fat molecules -- identified through modeling performed on DOE's Jaguar supercomputer.

"Using this approach, our preliminary work with CALB suggested that such a technique of introducing a compound that undergoes a light-inducible conformational change onto the surface of the protein could be used to manipulate enzyme reaction," Agarwal said.

While the researchers obtained final laboratory results at industry partner AthenaES, computational modeling allowed Agarwal to test thousands of combinations of enzyme sites, modification chemistry, different wavelengths of light, different temperatures and photo-activated switches. Simulations performed on Jaguar also allowed researchers to better understand how the enzyme's internal motions control the catalytic activity.

"This modeling was very important as it helped us identify regions of the enzyme that were modified by interactions with chemicals," said Agarwal, a member of ORNL's Computer Science and Mathematics Division. "Ultimately, the modeling helped us understand how the mechanical energy from the surface can eventually be transferred to the active site where it is used to conduct the chemical reaction."

Agarwal noted that enzymes are present in every organism and are widely used in industry as catalysts in the production of biofuels and countless other every day products. Researchers believe this finding could have immense potential for improving enzyme efficiency, especially as it relates to biofuels.


'/>"/>

Contact: Ron Walli
wallira@ornl.gov
865-576-0226
DOE/Oak Ridge National Laboratory
Source:Eurekalert

Related biology news :

1. Study resolves debate on human cell shut-down process
2. Antioxidant may disrupt Alzheimers disease process
3. KIT: Processes at the surface of catalysts
4. Study shows unified process of evolution in bacteria and sexual eukaryotes
5. Does depression contribute to the aging process?
6. Van Andel research institute study provides new details of fundamental cellular process
7. Cellular processing of proteins found in Congolese child birthing tea now revealed
8. NIH answers call to streamline technology transfer process
9. Trees adapt to poor levels of sunlight to effectively process carbon, study shows
10. USC researchers discover key aspect of process that activates breast cancer genes
11. UCLA biologists slow the aging process in fruit flies
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/16/2017)... , May 16, 2017  Veratad Technologies, LLC ... of online age and identity verification solutions, announced today ... Identity Conference 2017, May 15 thru May 17, 2017, ... Building and International Trade Center. Identity ... globe and in today,s quickly evolving digital world, defining ...
(Date:4/19/2017)... New York , April 19, 2017 ... competitive, as its vendor landscape is marked by the ... the market is however held by five major players ... Safran. Together these companies accounted for nearly 61% of ... of the leading companies in the global military biometrics ...
(Date:4/13/2017)... According to a new market research report "Consumer IAM Market ... Authorization), Service, Authentication Type, Deployment Mode, Vertical, and Region - Global Forecast ... from USD 14.30 Billion in 2017 to USD 31.75 Billion by 2022, ... ... MarketsandMarkets Logo ...
Breaking Biology News(10 mins):
(Date:5/23/2017)... ... May 23, 2017 , ... Customers often ... control technology again and again. METTLER TOLEDO has released two new videos that ... illustrate how integration of the ACT350 into Siemens and Allen Bradley PLCs is ...
(Date:5/22/2017)... Pittsburgh, PA (PRWEB) , ... May 22, 2017 ... ... Inc. announced today that it is exhibiting in booth B2 at the Association ... in Pittsburgh, May 22-25. , In addition to demonstrating its Cancer Diagnostic ...
(Date:5/19/2017)... Shirley, New York (PRWEB) , ... May 19, 2017 , ... ... therapy techniques in treating gait disorders, Biodex Medical Systems, Inc. announces the release of ... time music therapy has been joined with a biomedical system to aid in rehabilitating ...
(Date:5/18/2017)... ... May 18, 2017 , ... ... Tapas Cooking Challenge is a two-hour team-building package designed for groups of 10-30 ... Chef Jodi Abel, which include items, such as Blackened Shrimp with Edamame Salad, ...
Breaking Biology Technology: