Navigation Links
Novel test streamlines testing for Huntington Disease
Date:2/12/2013

f Utah, Salt Lake City, UT. Normal samples were included as well as those with a wide range of CAG repeats. The samples were blinded and analyzed.

The results showed that TP PCR correctly sized 240 of the 246 samples. All of the 100 samples in the normal and low risk groups were correctly sized. In the 146 samples of those known to be affected by HD (those with > 39 CAG repeats), the results for 140 correctly matched that found with other methods whereas the number of CAG repeats differed by 1 in 6 samples, a difference said by the authors to be within the precision of the method at higher repeat numbers. Up to 101 CAG repeats could be accurately sized with this test. Even samples that were found to be challenging to analyze with other methods could be assessed solely and accurately by TP PCR.

Another advantage of this new method is its ability to identify true homozygous normal samples, thus avoiding further testing. With other methodologies, if a sample appears homozygous for the normal allele, additional testing, often with Southern blot analysis, is still recommended because of the risk of false negatives. "Southern blotting is expensive, labor intensive, requires high concentrations of DNA, and can delay turnaround time," says Dr. Lyon. However, when HD is suspected in children, Dr. Lyon and colleagues recommend that even with TP PCR, apparently homozygous samples should undergo further testing.

TP PCR uses a forward and reverse chimeric primer to amplify from multiple priming sites within the trinucleotide repeat. TP PCR produces a characteristic ladder on a fluorescence electropherogram that allows the rapid and inexpensive identification and quantification of expanded repeats. Major peaks and minor peaks (stutters) representing CAG repeats can be analyzed and sized automatically using commercially available software.


'/>"/>

Contact: David Sampson
jmdmedia@elsevier.com
215-239-3171
Elsevier Health Sciences
Source:Eurekalert

Page: 1 2

Related biology news :

1. New tool for mining bacterial genome for novel drugs
2. Novel aptamer boosts T cell-based immune response to therapeutic vaccines
3. Novel technique reveals dynamics of telomere DNA structure
4. Novel approach to track migration of arctic-breeding avian species
5. USDA explores using novel genetic labs for faster detection of E. coli
6. Scientists develop novel method to study parasite numbers in wild seabirds
7. Gladstone scientists discover novel mechanism by which calorie restriction influences longevity
8. Research shows immune system response is detrimental to novel brain cancer therapy
9. A novel therapeutic advancement in the search for heart muscle progenitor cells
10. Texas Biomed files patent for a novel HIV vaccine strategy
11. Location, location, location: Membrane residence gives proteases novel abilities
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/30/2014)... reefs face a suite of perilous threats in ... development and climate change, fragile coral ecosystems are ... this trend, some species of corals surrounding the ... natural protector in their tropical environment: coral guard-crabs. ... History,s Smithsonian Marine Station scientist Seabird McKeon and ...
(Date:9/29/2014)... A species of gut bacteria called Clostridium ramosum , ... weight. The work is published this week in mBio ... Microbiology. , A research team from the German Institute ... human gut bacteria including C. ramosum gained weight ... C. ramosum were less obese even when consuming ...
(Date:9/29/2014)... September 29: Between 1970 and 2010 populations of ... globe dropped 52 percent, says the 2014 Living ... (WWF). This biodiversity loss occurs disproportionately in low-income ... high-income countries. , In addition to the precipitous ... to other warning signs about the overall health ...
Breaking Biology News(10 mins):Smithsonian scientists discover coral's best defender against an army of sea stars 2Gut bacteria promote obesity in mice 2Half of global wildlife lost, says new WWF report 2
... Philadelphia, PA, December 12, 2012 Cell aging, or ... physiological response to tumor development. Activated oncogenes are able ... oncogene-induced senescence (OIS) could play a key role in ... unknown mechanism in the regulation of OIS. This study ...
... WINSTON-SALEM, N.C., Dec. 11, 2012 When Teresa Thorn started ... something she,d had years earlier. As the days ... changing, something her co-workers noticed. Finally one Sunday, she and ... Wake Forest Baptist Medical Center, where a battery of tests ...
... research, presented this morning at the 54th Annual ... identified important associations between Plasmodium falciparum ... help researchers identify young children who are more susceptible ... malaria infection alone was considered the important factor, this ...
Cached Biology News:Previously unknown mechanism identified in oncogene-induced senescence 2Vaccine offers promise as post-surgery treatment for pancreatic cancer patients 2Vaccine offers promise as post-surgery treatment for pancreatic cancer patients 3Vaccine offers promise as post-surgery treatment for pancreatic cancer patients 4New research helps predict susceptibility to Burkitt lymphoma 2
(Date:9/30/2014)... Follow us on LinkedIn –Cholesterol ... cholesterol carrier or lipoprotein in the blood. Such tests ... in the body, especially along the walls of blood ... other cardiovascular disorders. Cardiovascular Diseases (CVD) is the leading ... on a regular basis, to assess an individual’s risk ...
(Date:9/30/2014)... 2014 CORD:USE Cord Blood Bank – ... has entered into an Equity and Exclusive Services Agreement ... series of patented technologies for the isolation of human ... Tianhe is performing Phase I/II clinical trials in ... using Tianhe,s Stem Cell Educator Therapy showing that ...
(Date:9/30/2014)... Switzerland (PRWEB) September 30, 2014 ... software solutions for drug discovery and related ... FUNGITECT consortium has chosen Genedata Selector as ... sequencing (NGS) data. FUNGITECT is a European ... invasive fungal diseases via individualized anti-fungal drug ...
(Date:9/30/2014)...  KemPharm, Inc., a clinical-stage specialty pharmaceutical company ... new molecular entity (NME) prodrugs, announced today that ... issued U.S. Patent No. 8,816,083 to KemPharm for ... Derivatives and Heteroaryl Carboxylic Acid Conjugates of Hydromorphone, ... The patent, which extends through 2032, provides ...
Breaking Biology Technology:Surging Diabetic, Obese and Hypertensive Population Drives the Global Cholesterol Testing Market, According to New Report by Global Industry Analysts, Inc. 2Surging Diabetic, Obese and Hypertensive Population Drives the Global Cholesterol Testing Market, According to New Report by Global Industry Analysts, Inc. 3Surging Diabetic, Obese and Hypertensive Population Drives the Global Cholesterol Testing Market, According to New Report by Global Industry Analysts, Inc. 4CORD:USE is Pleased to Announce it has Partnered with Tianhe Stem Cell Biotechnologies, Developer of Cord Blood Stem Cell Therapy Showing Promising Results in Clinical Trials for the Treatment of Diabetes and Autoimmune Diseases 2CORD:USE is Pleased to Announce it has Partnered with Tianhe Stem Cell Biotechnologies, Developer of Cord Blood Stem Cell Therapy Showing Promising Results in Clinical Trials for the Treatment of Diabetes and Autoimmune Diseases 3CORD:USE is Pleased to Announce it has Partnered with Tianhe Stem Cell Biotechnologies, Developer of Cord Blood Stem Cell Therapy Showing Promising Results in Clinical Trials for the Treatment of Diabetes and Autoimmune Diseases 4FUNGITECT Consortium Adopts Genedata Selector as Data Analysis Platform for Anti-fungal R 2FUNGITECT Consortium Adopts Genedata Selector as Data Analysis Platform for Anti-fungal R 3KemPharm, Inc. Receives Patent from the USPTO for Novel Prodrug of Hydromorphone, KP511 2
... Non-HDL and apoB measurement helps physicians guide therapy for ... cardiometabolic ... of LDL,non-HDL and apoB are now recognized as valid, accurate ... for patients with,cardiometabolic risk (CMR). The VAP(R) Cholesterol Test (Atherotech, ...
... - CuraGen to receive approximately $45 million in cash, stock and future ... milestone payments ... Provides updated financial guidance for 2008 - ... BRANFORD, Conn., April 22 CuraGen Corporation,(Nasdaq: CRGN ), a clinical-stage ...
... April 22 /Xinhua-PRNewswire/ -- Fufeng Group Limited,("Fufeng" or ... "the Group"; HKEx: 546), a leading vertically integrated,manufacturer ... results for the year ended 31 December 2007., ... sales volume of major,products, the Group,s turnover increased ...
Cached Biology Technology:Atherotech VAP Cholesterol Test Meets Updated ADA, ACC Measurement and Treatment Guidelines for High Risk Patients 2Atherotech VAP Cholesterol Test Meets Updated ADA, ACC Measurement and Treatment Guidelines for High Risk Patients 3CuraGen Sells Ownership of Belinostat to TopoTarget A/S 2CuraGen Sells Ownership of Belinostat to TopoTarget A/S 3CuraGen Sells Ownership of Belinostat to TopoTarget A/S 4CuraGen Sells Ownership of Belinostat to TopoTarget A/S 5Fufeng Announces 2007 Annual Results 2Fufeng Announces 2007 Annual Results 3
MOUSE ANTI BOVINE TROPONIN I...
Ready-to-use; 40mg/ml X-Gal and 32mg/ml IPTG...
Normal donkey serum collected from healthy normal donkeys....
This BioSolution package is excellent for doing fluorescence thermal melt experiments on nucleic acids, studies involving biomolecular interactions, protein folding experiments, and more!...
Biology Products: