Navigation Links
Novel technique to produce stem cells from peripheral blood
Date:11/1/2012

Stem cells are a valuable resource for medical and biological research, but are difficult to study due to ethical and societal barriers. However, genetically manipulated cells from adults may provide a path to study stem cells that avoid any ethical concerns. A new video-protocol in JoVE (Journal of Visualized Experiments), details steps to generate human induced pluripotent stem cells (iPSC) from cells in the peripheral blood. The technique has been developed by Boston University's Dr. Gustavo Mostoslavsky and his colleagues.

Stem cells are unique because they can self-renew, differentiate into multiple cell types (pluripotency), and are immortal. Fertilized eggs produce embryonic stem cells with the potential to differentiate into any other cell type. Despite their value, embryonic stem cells pose a variety of ethical, legal and political implications that cause scientists to look for less controversial paths of study. "As opposed to human embryonic stem cells that originate in fertilized eggs, human induced pluripotent stem cells can be generated from any cell, and there are no ethical barriers to this," Dr. Mostoslavsky explains.

"Our article describes a methodology to obtain high quality, induced pluripotent stem cell lines," Dr. Mostoslavsky continues. His work is particularly interesting to the stem cell research community because "blood is an easily accessible sample for most laboratories. Our procedure uses a vector we created and produced a few years ago, and is very efficient. Our colleagues are very interested in that high efficiency. We published in JoVE because there are some technical details in the protocol that are best conveyed with a video-protocol. "

Dr. Mostoslavsky uses induced pluripotent stem cells to model genetic lung diseases, providing a fast track to study rare diseases in the laboratory. This technique will prove a valuable time and cost saving resource for other scientists interested in genetic disorders. The video-protocol was published in JoVE on October 31, 2012. JoVE Senior Science Editor Dr. Nandita Singh tells us, "We are excited to describe the generating of human iPSC using a lentiviral vector the Yamanaka factors. This vector was developed in Dr. Mostoslavsky's lab. The ease of obtaining the tissue sample combined with the high efficiency of reprogramming makes this method a very valuable tool in the field. The JoVE format will help the scientific community to accurately replicate this methodology.


'/>"/>

Contact: Neal Moawed
press@jove.com
617-245-0137
The Journal of Visualized Experiments
Source:Eurekalert

Related biology news :

1. Researchers discover novel therapy for Crohns disease
2. New clinical trial explores novel noninvasive colon cancer screening test
3. Notre Dame researchers using novel method to combat malaria drug resistance
4. Unique adaptations to a symbiotic lifestyle reveal novel targets for aphid insecticides
5. Key proteins newly discovered form and function may provide novel cancer treatment target
6. Novel discovery by NUS scientists paves the way for more effective treatment of cancers
7. London researchers discover novel mechanism involved in key immune response
8. Mexican rock heroes trial novel green trading system
9. VTT and GE Healthcare developing novel biomarkers to predict Alzheimers disease
10. Cleveland Clinic researchers receive $5 million grant to discover novel pathways to heart disease
11. Novel nano-structures to realize hydrogens energy potential
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/3/2017)... , April 3, 2017  Data captured ... engineering platform, detected a statistically significant association ... prior to treatment and objective response of ... potential to predict whether cancer patients will ... treatment, as well as to improve both pre-infusion ...
(Date:3/30/2017)... 2017 Trends, opportunities and forecast in this ... technology (fingerprint, AFIS, iris recognition, facial recognition, hand geometry, ... end use industry (government and law enforcement, commercial and ... and others), and by region ( North America ... Asia Pacific , and the Rest of the ...
(Date:3/27/2017)... CENTRE, N.Y. , March 27, 2017 /PRNewswire-USNewswire/ ... Healthcare Information and Management Systems Society (HIMSS) Analytics ... Outpatient EMR Adoption Model sm . In addition, ... 12% of U.S. hospitals using an electronic medical ... CHS for its high level of EMR usage ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... ... launched Rosalind™, the first-ever genomics analysis platform specifically designed for life science ... in honor of pioneering researcher Rosalind Franklin, who made a major contribution ...
(Date:10/11/2017)... ... 11, 2017 , ... The CRISPR-Cas9 system has ... and avoiding the use of exogenous expression plasmids. The simplicity of programming this ... gain-of-function studies. , This complement to loss-of-function studies, such as with RNAi ...
(Date:10/11/2017)... ... , ... ComplianceOnline’s Medical Device Summit is back for its 4th year. The ... Francisco, CA. The Summit brings together current and former FDA office bearers, regulators, industry ... officials from around the world to address key issues in device compliance, quality and ...
(Date:10/11/2017)... INDIANAPOLIS , Oct. 11, 2017  VMS BioMarketing, a ... of a nationwide oncology Clinical Nurse Educator (CNE) network, which ... growing need for communication among health care professionals to enhance ... physicians, nurses, office staff, and other health care professionals to ... for breast cancer. ...
Breaking Biology Technology: