Navigation Links
Novel technique to produce stem cells from peripheral blood

Stem cells are a valuable resource for medical and biological research, but are difficult to study due to ethical and societal barriers. However, genetically manipulated cells from adults may provide a path to study stem cells that avoid any ethical concerns. A new video-protocol in JoVE (Journal of Visualized Experiments), details steps to generate human induced pluripotent stem cells (iPSC) from cells in the peripheral blood. The technique has been developed by Boston University's Dr. Gustavo Mostoslavsky and his colleagues.

Stem cells are unique because they can self-renew, differentiate into multiple cell types (pluripotency), and are immortal. Fertilized eggs produce embryonic stem cells with the potential to differentiate into any other cell type. Despite their value, embryonic stem cells pose a variety of ethical, legal and political implications that cause scientists to look for less controversial paths of study. "As opposed to human embryonic stem cells that originate in fertilized eggs, human induced pluripotent stem cells can be generated from any cell, and there are no ethical barriers to this," Dr. Mostoslavsky explains.

"Our article describes a methodology to obtain high quality, induced pluripotent stem cell lines," Dr. Mostoslavsky continues. His work is particularly interesting to the stem cell research community because "blood is an easily accessible sample for most laboratories. Our procedure uses a vector we created and produced a few years ago, and is very efficient. Our colleagues are very interested in that high efficiency. We published in JoVE because there are some technical details in the protocol that are best conveyed with a video-protocol. "

Dr. Mostoslavsky uses induced pluripotent stem cells to model genetic lung diseases, providing a fast track to study rare diseases in the laboratory. This technique will prove a valuable time and cost saving resource for other scientists interested in genetic disorders. The video-protocol was published in JoVE on October 31, 2012. JoVE Senior Science Editor Dr. Nandita Singh tells us, "We are excited to describe the generating of human iPSC using a lentiviral vector the Yamanaka factors. This vector was developed in Dr. Mostoslavsky's lab. The ease of obtaining the tissue sample combined with the high efficiency of reprogramming makes this method a very valuable tool in the field. The JoVE format will help the scientific community to accurately replicate this methodology.


Contact: Neal Moawed
The Journal of Visualized Experiments

Related biology news :

1. Researchers discover novel therapy for Crohns disease
2. New clinical trial explores novel noninvasive colon cancer screening test
3. Notre Dame researchers using novel method to combat malaria drug resistance
4. Unique adaptations to a symbiotic lifestyle reveal novel targets for aphid insecticides
5. Key proteins newly discovered form and function may provide novel cancer treatment target
6. Novel discovery by NUS scientists paves the way for more effective treatment of cancers
7. London researchers discover novel mechanism involved in key immune response
8. Mexican rock heroes trial novel green trading system
9. VTT and GE Healthcare developing novel biomarkers to predict Alzheimers disease
10. Cleveland Clinic researchers receive $5 million grant to discover novel pathways to heart disease
11. Novel nano-structures to realize hydrogens energy potential
Post Your Comments:
(Date:5/16/2016)... , May 16, 2016   EyeLock LLC , ... announced the opening of an IoT Center of Excellence ... and expand the development of embedded iris biometric applications. ... level of convenience and security with unmatched biometric accuracy, ... identity aside from DNA. EyeLock,s platform uses video technology ...
(Date:4/28/2016)... 28, 2016 First quarter 2016:   ... compared with the first quarter of 2015 The gross ... M (loss: 18.8) and the operating margin was 40% (-13) ... Cash flow from operations was SEK 249.9 M (21.2) , ... is unchanged, SEK 7,000-8,500 M. The operating margin for ...
(Date:4/15/2016)... Research and Markets has announced ... 2016-2020,"  report to their offering.  , ... global gait biometrics market is expected to grow ... 2016-2020. Gait analysis generates multiple variables ... to compute factors that are not or cannot ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... -- Global demand for enzymes is forecast to grow ... billion.  This market includes enzymes used in industrial ... animal feed, and other markets) and specialty applications ... beverages will remain the largest market for enzymes, ... containing enzymes in developing regions.  These and other ...
(Date:6/27/2016)... , ... June 27, 2016 , ... Parallel 6 ... trials, announced today the Clinical Reach Virtual Patient Encounter CONSULT module which ... with the physician and clinical trial team. , Using the CONSULT module, patients and ...
(Date:6/27/2016)... , June 27, 2016   Ginkgo Bioworks ... to industrial engineering, was today awarded as one ... selection of the world,s most innovative companies. Ginkgo ... scale for the real world in the nutrition, ... engineers work directly with customers including Fortune 500 ...
(Date:6/24/2016)... ... June 24, 2016 , ... Researchers at the Universita ... miRNAs in people with peritoneal or pleural mesothelioma. Their findings are the subject of ... now. , Diagnostic biomarkers are signposts in the blood, lung fluid or tissue ...
Breaking Biology Technology: