Navigation Links
Novel technique reveals dynamics of telomere DNA structure
Date:1/17/2013

s the G-quadruplex to unfold.

"Unlike other DNA structures, the G-quadruplex structure is fairly brittle. It takes very little perturbation to make the whole thing fall apart," Stone said. "We also found that the unfolded state has a highly compacted conformation, which tells us that it still has interactions that favor the folding reaction."

These findings have implications for understanding the molecular mechanisms of telomere-associated proteins and enzymes involved in the unfolding reaction, as well as for rational design of anti-cancer drugs, Stone said. Small molecules that bind to and stabilize telomere DNA G-quadruplexes have shown promise as anti-cancer drugs.

The integration of fluorescence measurements and magnetic tweezers is a powerful method for monitoring DNA structural dynamics, and as biophysical techniques go, it is not hard to implement, Stone said. His lab worked with DNA molecules containing the G-quadruplex sequence from human telomere DNA, attaching one end of the DNA to a glass slide and the other end to a tiny magnetic bead. A magnet held above the sample pulled on the bead, exerting a stretching force on the DNA molecule that varied according to how close the magnet was to the sample.

At the same time, the researchers used a fluorescence technique called single-molecule FRET (Frster resonance energy transfer) to monitor small-scale structural changes in the DNA. "FRET can be thought of as a molecular ruler," Stone said. As energy from one fluorescent dye molecule is transferred to a second dye molecule, the efficiency of the energy transfer can be measured in real time. The dye molecules can be coupled directly to the DNA molecule at specific sites, allowing researchers to monitor the molecular dynamics of the system as it is being manipulated by the magnetic tweezers.

"You don't have to be a specialist to use this technique, so it can be easily transferred to other labs and broadly employed i
'/>"/>

Contact: Tim Stephens
stephens@ucsc.edu
831-459-2495
University of California - Santa Cruz
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Researchers discover novel therapy for Crohns disease
2. New clinical trial explores novel noninvasive colon cancer screening test
3. Notre Dame researchers using novel method to combat malaria drug resistance
4. Unique adaptations to a symbiotic lifestyle reveal novel targets for aphid insecticides
5. Key proteins newly discovered form and function may provide novel cancer treatment target
6. Novel discovery by NUS scientists paves the way for more effective treatment of cancers
7. London researchers discover novel mechanism involved in key immune response
8. Mexican rock heroes trial novel green trading system
9. VTT and GE Healthcare developing novel biomarkers to predict Alzheimers disease
10. Cleveland Clinic researchers receive $5 million grant to discover novel pathways to heart disease
11. Novel nano-structures to realize hydrogens energy potential
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/31/2014)... is developing drugs that are highly effective against a ... patient. Such properties are directly related to the 3D ... have a shape that is perfectly complementary to a ... specificity. Publishing in Nature Chemistry , EPFL scientists ... the 3D structure of bioactive peptides and enhance their ...
(Date:8/31/2014)... Nature Climate Change , suggests that if current ... exceed, the global targets for total greenhouse gas (GHG) emissions ... all think carefully about the food we choose and its ... is just one of a number of actions that need ... there is enough food for all. , As populations ...
(Date:8/29/2014)... have made some surprising discoveries about the body,s ... virus (SIV), the team found that specialized cells ... responders to viral invasion and are the source ... interleukin-1 beta (IL-1β). , Though aimed at ... the gut epithelium that provides a barrier to ...
Breaking Biology News(10 mins):A new synthetic amino acid for an emerging class of drugs 2A new synthetic amino acid for an emerging class of drugs 3Changing global diets is vital to reducing climate change 2Changing global diets is vital to reducing climate change 3Changing global diets is vital to reducing climate change 4The early cost of HIV 2
... in billions, sometimes - like the snow bunting ... distances and enduring the most extreme frigid weather conditions. ... a stunning success of biological adaptation with Snow ... north as the Arctic Circle. Indeed, there is nothing ...
... be a household name even among farm families, but ... environmentally-friendly commercial biofuel feedstock, particularly for biodiesel and jet ... has just awarded $5.08 million to a team of ... Grain Science and Industry, Xiuzhi "Susan" Sun, to study ...
... coughs, soot or black carbon turns out to ... only to carbon dioxide, according to a four-year assessment by ... the soot particles in smoke and smog, contributes about twice ... the 2007 Intergovernmental Panel on Climate Change. "We were surprised ...
Cached Biology News:Novel approach to track migration of arctic-breeding avian species 2Novel approach to track migration of arctic-breeding avian species 3Researcher awarded $5.08 million to study oilseed camelina as biofuel feedstock 2Researcher awarded $5.08 million to study oilseed camelina as biofuel feedstock 3International study: Where there's smoke or smog, there's climate change 2International study: Where there's smoke or smog, there's climate change 3
(Date:8/29/2014)... San Antonio, TX (PRWEB) August 29, 2014 ... 9001:2008 and ISO 13485:2003 certified, GAMP® 5 compliant ... been awarded an international Phase II clinical trial ... non-Hodgkin’s lymphoma. , Throughout this trial, Intrinsic Imaging ... but not limited to, protocol and charter development, ...
(Date:8/28/2014)... 2014 Best Sanitizers, Inc., a leader ... processing industry, is asking industry professionals to prepare for ... the E2 soap they’re currently using to the Best ... Hand hygiene is critical to fighting cross-contamination and ... Six key criteria are identified to evaluate the effectiveness ...
(Date:8/28/2014)... Lawrence, MA (PRWEB) August 28, 2014 “This ... single test to screen from 0 to 150 ppb,” said ... test is a significant benefit to plant owners and USDA-GIPSA ... corn and other commodities. Testing can take place in a ... Not only can the plant test the feed and grain ...
(Date:8/28/2014)... important devices in spintronics, an electronic which is ... but also on their spin and the spin-related ... into magnetic signals and vice versa. Recently, the ... Institute of Physics at Johannes Gutenberg University Mainz ... and Japan, has for the first time realised ...
Breaking Biology Technology:Intrinsic Imaging Awarded Phase II Clinical Trial to Assess New Treatment for Non-Hodgkin’s Lymphoma 2Best Sanitizers, Inc. Asks Food Industry Professionals: With Fall Harvest On the Way, Is Your E2 Hand Soap Up to the Task? 2A new, tunable device for spintronics 2A new, tunable device for spintronics 3
... Neuftec,Limited, the proprietor of the original nano-particulate ... litigation in the,UK against Oxonica Energy Limited, a ... issued in the High Court of Justice, allege ... an injunction against Oxonica as well as damages,in ...
... Senesco,Technologies, Inc. ("Senesco" or the "Company") (Amex: ... presenting at the Business Forum during BIO 2008,the ... held at the San Diego Convention Center from ... Senesco, will deliver the,Company,s corporate presentation on Wednesday, ...
... CHURCH, Va., June 12 CSC (NYSE: ... HealthTrio LLC to add portal and personal,health record ... and other payer clients. The added components include ... October 2007, FirstPortfolio provides users with a,collection of ...
Cached Biology Technology:Neuftec Issues Patent Infringement Proceedings in the UK Against Oxonica 2Senesco Technologies to Present at BIO 2008 Business Forum 2CSC and HealthTrio Team to Offer Portal and Personal Health Record Solution 2CSC and HealthTrio Team to Offer Portal and Personal Health Record Solution 3
... Glycosylation is a post-translational modification ... biological functions, including immune regulation, inflammation, ... Biotechnology has developed two lectin based ... and Wheat Germ Agglutinin (WGA), that ...
... carrier proteins to simplify the production ... Carrier Proteins are commonly used carriers ... heterobifunctional cross-linker (Sulfo-SMCC). This activation results ... Thus allowing the maleimide-activated carrier ...
Cell Culture Flask, 25 cm, tissue-culture treated polystyrene...
Cell Culture Flask, nontreated polystyrene...
Biology Products: