Navigation Links
Novel technique reveals dynamics of telomere DNA structure
Date:1/17/2013

s the G-quadruplex to unfold.

"Unlike other DNA structures, the G-quadruplex structure is fairly brittle. It takes very little perturbation to make the whole thing fall apart," Stone said. "We also found that the unfolded state has a highly compacted conformation, which tells us that it still has interactions that favor the folding reaction."

These findings have implications for understanding the molecular mechanisms of telomere-associated proteins and enzymes involved in the unfolding reaction, as well as for rational design of anti-cancer drugs, Stone said. Small molecules that bind to and stabilize telomere DNA G-quadruplexes have shown promise as anti-cancer drugs.

The integration of fluorescence measurements and magnetic tweezers is a powerful method for monitoring DNA structural dynamics, and as biophysical techniques go, it is not hard to implement, Stone said. His lab worked with DNA molecules containing the G-quadruplex sequence from human telomere DNA, attaching one end of the DNA to a glass slide and the other end to a tiny magnetic bead. A magnet held above the sample pulled on the bead, exerting a stretching force on the DNA molecule that varied according to how close the magnet was to the sample.

At the same time, the researchers used a fluorescence technique called single-molecule FRET (Frster resonance energy transfer) to monitor small-scale structural changes in the DNA. "FRET can be thought of as a molecular ruler," Stone said. As energy from one fluorescent dye molecule is transferred to a second dye molecule, the efficiency of the energy transfer can be measured in real time. The dye molecules can be coupled directly to the DNA molecule at specific sites, allowing researchers to monitor the molecular dynamics of the system as it is being manipulated by the magnetic tweezers.

"You don't have to be a specialist to use this technique, so it can be easily transferred to other labs and broadly employed i
'/>"/>

Contact: Tim Stephens
stephens@ucsc.edu
831-459-2495
University of California - Santa Cruz
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Researchers discover novel therapy for Crohns disease
2. New clinical trial explores novel noninvasive colon cancer screening test
3. Notre Dame researchers using novel method to combat malaria drug resistance
4. Unique adaptations to a symbiotic lifestyle reveal novel targets for aphid insecticides
5. Key proteins newly discovered form and function may provide novel cancer treatment target
6. Novel discovery by NUS scientists paves the way for more effective treatment of cancers
7. London researchers discover novel mechanism involved in key immune response
8. Mexican rock heroes trial novel green trading system
9. VTT and GE Healthcare developing novel biomarkers to predict Alzheimers disease
10. Cleveland Clinic researchers receive $5 million grant to discover novel pathways to heart disease
11. Novel nano-structures to realize hydrogens energy potential
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/22/2014)... Society of Environmental Toxicology and Chemistry (SETAC) held a ... the scientific research needed to understand the risks of ... The effort was extended, and results were published ... journal, Integrated Environmental Assessment and Management (IEAM). ... interviewing the lead author of the study, Murray Rudd ...
(Date:9/22/2014)... homes, if they could locate people, places and objects. ... lasers, which have difficulty reliably recognizing things and can ... way robots can "sense" what is around them is ... (UHF RFID) tags. Inexpensive self-adhesive tags can be stuck ... room for the correct tag,s signal, even when the ...
(Date:9/22/2014)... More than 7,000 geoscientists will gather in Vancouver, ... Geological Society of America,s 126th Annual Meeting & Exposition ... topics as the transportation of crude oil, earthquake prediction ... field guide, Trials and Tribulations of Life on an ... the Vancouver area. , A broad range of topics ...
Breaking Biology News(10 mins):Priorities for research on pharmaceutical and personal care products in the environment 2New RFID technology helps robots find household objects 2New RFID technology helps robots find household objects 3Geoscience topics in the spotlight -- Vancouver, BC 2Geoscience topics in the spotlight -- Vancouver, BC 3Geoscience topics in the spotlight -- Vancouver, BC 4Geoscience topics in the spotlight -- Vancouver, BC 5
... and other parts of the U.S. plus increasing worldwide ... Texas AgriLife Research and Extension Center in Uvalde even ... includes develop methods of augmenting aquifers, ways to determine ... water taken from them," said Dr. Bill Holloway of ...
... team of scientists from the National Institute of Oceanography ... expedition on research vessel Polarstern. The cooperative project Lohafex ... But it has dampened hopes on the potential of ... dioxide (CO 2 ) and thus mitigate global warming. ...
... a unique technique for sensing motion may inspire a new ... Although members of the fish species ... the movement of water around them with gel-covered hairs that ... and navigate through their lightless environment inspired a group of ...
Cached Biology News:Uvalde Center water research could have national, international applications 2Uvalde Center water research could have national, international applications 3Lohafex provides new insights on plankton ecology 2Lohafex provides new insights on plankton ecology 3Lohafex provides new insights on plankton ecology 4Researchers develop flow sensors based on hair structures of blind cavefish 2Researchers develop flow sensors based on hair structures of blind cavefish 3
(Date:9/23/2014)... Dr. Williams says, “Find The Black ... offers a three phase process that can lead to ... that would go far beyond anything previously imagined. First ... of healthcare.” , “Every effort to improve the quality ... discernable progress throughout that system because all of the ...
(Date:9/23/2014)... England , September 23, 2014 ... launched with funding from Imperial Innovations, Cambridge Innovation Capital ... Inivata, a clinical cancer genomics company focused on ... analysis to improve cancer testing and treatment, today ... round led by Imperial Innovations and including Cambridge ...
(Date:9/22/2014)... and SANTA CLARA, Calif. , Sept. ... WBMD ), the leading source of health information, ... survey that provide novel insights regarding physician and ... and care.  Dr. Eric Topol , a leading ... who serves as both Editor-in-Chief of Medscape and Chief ...
(Date:9/22/2014)... it is to stop bleeding, are very simple. They ... the physical environment around them, researchers at Emory and ... with greater stiffness by increasing their stickiness, the degree ... components of the clotting system, the researchers found. , ... in that they are able to sense the physical ...
Breaking Biology Technology:Dr. Ira Williams Offers the Healthcare Solution Necessary for Patient Safety Improvement 2Inivata Raises £4m to Realise the Potential of ctDNA Analysis to Improve Cancer Treatment Through Simple Blood Tests 2Inivata Raises £4m to Realise the Potential of ctDNA Analysis to Improve Cancer Treatment Through Simple Blood Tests 3Inivata Raises £4m to Realise the Potential of ctDNA Analysis to Improve Cancer Treatment Through Simple Blood Tests 4WebMD/Medscape Digital Technology Survey Reveals Unique Insights Into How Patients and Physicians Perceive the Role, Potential and Risks Associated with Digital Health Technologies 2WebMD/Medscape Digital Technology Survey Reveals Unique Insights Into How Patients and Physicians Perceive the Role, Potential and Risks Associated with Digital Health Technologies 3WebMD/Medscape Digital Technology Survey Reveals Unique Insights Into How Patients and Physicians Perceive the Role, Potential and Risks Associated with Digital Health Technologies 4Platelets modulate clotting behavior by 'feeling' their surroundings 2
... NexBio announced top line data from a Phase 2 ... and prophylaxis of Influenza-Like Illness (ILI) caused by all ... pandemic influenza strains. In a double-blind, placebo-controlled, Phase 2 ... treatment with DAS181 resulted in a significant reduction in ...
... that takes just fifteen minutes to analyze a sample ... US researchers. The technology amplifies any anthrax DNA present ... just 40 microscopic cells of the deadly bacteria Bacillus ... a potentially lethal microbe that might be used intentionally ...
... globe, many nations are realizing that the potential for ... And because an intentional introduction of bacteria, a virus ... for Animal Health is issuing a paper aimed at ... think about maintaining international trade needs to be aware ...
Cached Biology Technology:NexBio Reports Safety and Significant Antiviral Activities of DAS181 (Fludase)* from a Dose-Ranging Phase II Clinical Trial 2NexBio Reports Safety and Significant Antiviral Activities of DAS181 (Fludase)* from a Dose-Ranging Phase II Clinical Trial 3NexBio Reports Safety and Significant Antiviral Activities of DAS181 (Fludase)* from a Dose-Ranging Phase II Clinical Trial 4Nano detector for deadly anthrax 2Global bioterrorism threat analyzed for world animal health office 2
... V is a fully integrated system for ... processing that deliver excellent quality microarray images. ... quality microarray scanner that is based on ... background rejection and spatial resolution. The DNAscope ...
Ca2+ Channel Ca gamma 2 Purified Anti-Mouse, Anti-Rat, Anti-Human clone Polyclonal, Isotype Rabbit Ig, 50 µl Consult technical datasheet for details....
Rabbit polyclonal antibody to NCOA2 [Agarose Immobilized]...
Monocarboxylate (lactate) transporter 2...
Biology Products: