Navigation Links
Novel microscopy method offers sharper view of brain's neural network
Date:8/23/2012

region that scientists have tried to survey in mice with LSM is the neural pathway, the billion-fold network of neurons that underlie the functioning of the brain. While the LSM method yields high-resolution views of tissue excised from mouse brains and fixed in position, whole brain samples scatter the emitted light and create background fluorescence that reduces contrast and blurs the perceived image. Obviously, this aberration makes it impossible to resolve and reconstruct the entire neuronal network with a high contrast.

To correct the problem, Pavone and his colleagues combined the advantages of light sheet illumination with confocal microscopy, an imaging method that uses a filter to remove photons that stray from the single plane of the thin sheet.

"We found that the combined system, which we call confocal light sheet microscopy or CLSM, filtered the scattered photons that were emitted and recovered the normally lost image contrast in real time without the need for multiple acquisitions or any post-processing of the acquired data," Pavone says. "This allows us to obtain sharp image slices that can be easily reconstructed into a macroscopic view of whole organs or entire organisms with a resolution of a few microns and faster acquisition time."

The images obtained with CLSM, Pavone says, are 100 percent sharper than those acquired with conventional LSM.

The researchers validated the background rejection capabilities of CLSM by showing that it results in significantly sharper images than conventional LSM microscopy and than a variation of LSM that requires redundant slices and post-processing to remove scattered light. CLSM proved superior to the other two methods when used to view three samples from mouse brains: the hippocampus, the cerebellum and the whole brain. The 3-D reconstruction ability of CLSM also was put to the test when the researchers used it to map the micron-scale neuroanatomy of mouse Purkinje cells (large
'/>"/>

Contact: Angela Stark
astark@osa.org
202-416-1443
Optical Society of America
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Novel nano-structures to realize hydrogens energy potential
2. Cleveland Clinic researchers receive $5 million grant to discover novel pathways to heart disease
3. VTT and GE Healthcare developing novel biomarkers to predict Alzheimers disease
4. Mexican rock heroes trial novel green trading system
5. London researchers discover novel mechanism involved in key immune response
6. Novel discovery by NUS scientists paves the way for more effective treatment of cancers
7. Key proteins newly discovered form and function may provide novel cancer treatment target
8. Unique adaptations to a symbiotic lifestyle reveal novel targets for aphid insecticides
9. Notre Dame researchers using novel method to combat malaria drug resistance
10. New clinical trial explores novel noninvasive colon cancer screening test
11. Researchers discover novel therapy for Crohns disease
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Novel microscopy method offers sharper view of brain's neural network
(Date:8/28/2014)... to Research Careers) Program has announced the travel award ... October 18 22, 2014 in San Diego, California. ... of students, post doctorates and scientists from underrepresented groups ... to encourage the participation of young scientists at the ... conferred 16 awards totaling $29,600., The FASEB MARC Program ...
(Date:8/28/2014)... developmental on-off switch for Streptomyces , a group ... the world,s naturally derived antibiotic medicines. , Their ... possible to manipulate this switch to make nature,s antibiotic ... 28 in Cell , found that a unique ... larger protein called BldD ultimately controls whether a bacterium ...
(Date:8/28/2014)... and imaging how quickly blood flows in the brain ... abuse affects the brain, which may aid in improving ... treatment options for recovering drug addicts. The new method, ... University in New York, USA and the U.S. National ... Society,s (OSA) open-access journal Biomedical Optics Express ...
Breaking Biology News(10 mins):Small molecule acts as on-off switch for nature's antibiotic factory 2Small molecule acts as on-off switch for nature's antibiotic factory 3This is your brain's blood vessels on drugs 2This is your brain's blood vessels on drugs 3
... dayand starting this week, they will be part of a multinational ... mines left over from as long ago as the First and ... naval exercises in the Baltic Sea this year, where more than ... all part of a day,s work for U.S. Navy divers, who ...
... in air pollution were associated with changes in biomarkers of ... as measures of cardiovascular physiology in healthy young persons, according ... JAMA, a theme issue on Global Health. ... but the mechanisms by which air pollution leads to CVD ...
... A review of studies reporting estimates of the ... malaria over the past 20 years suggests that a ... women attending antenatal (before birth) facilities in sub-Saharan Africa, ... published in the May 16 issue of JAMA ...
Cached Biology News:Undersea warriors, undersea medicine: The future force 2Air pollution level changes in Beijing linked with biomarkers of cardiovascular disease 2Air pollution level changes in Beijing linked with biomarkers of cardiovascular disease 3Air pollution level changes in Beijing linked with biomarkers of cardiovascular disease 4Considerable prevalence of both malaria, STIs exist among pregnant women in sub-Saharan Africa 2
(Date:8/28/2014)... this month, a report was created by Colorado ... Force on the performance of vehicles using an eco-friendly, ... fuel made from mustard seeds, offered great potential as ... friendly future. In fact, every branch of the U.S. ... foreign oil over the next few years. They will ...
(Date:8/27/2014)... (PRWEB) August 27, 2014 Varian Medical ... expand its current Salt Lake City manufacturing facility and ... of Utah to ultimately create 1,000 new full time ... , “Utah is home to hardworking people with ... be credited with Varian’s success in the manufacturing of ...
(Date:8/27/2014)... Aug. 27, 2014 Reportlinker.com announces ... available in its catalogue: Global ... http://www.reportlinker.com/p02316661/Global-Biodegradable-Packaging-Materials-Market-2014-2018.html About ... that are readily decomposed by the action ... non-degradable packaging in terms of raw materials, ...
(Date:8/27/2014)... NEW YORK , Aug. 27, 2014 /PRNewswire/ ... report is available in its catalogue: ... http://www.reportlinker.com/p02316662/Global-Chelating-Agents-Market-2014-2018.html About Chelating Agent ... can form covalent bonds with metal ions, thereby ... effect that some metal-ions have on chemical processes, ...
Breaking Biology Technology:Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 2Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 3Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 4Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 5Varian Medical Systems Groundbreaking on Facility Expansion Triggers Influx of New Jobs to Salt Lake 2Varian Medical Systems Groundbreaking on Facility Expansion Triggers Influx of New Jobs to Salt Lake 3Global Biodegradable Packaging Materials Market 2014-2018 2Global Biodegradable Packaging Materials Market 2014-2018 3Global Biodegradable Packaging Materials Market 2014-2018 4Global Biodegradable Packaging Materials Market 2014-2018 5Global Biodegradable Packaging Materials Market 2014-2018 6Global Biodegradable Packaging Materials Market 2014-2018 7Global Chelating Agents Market 2014-2018 2Global Chelating Agents Market 2014-2018 3Global Chelating Agents Market 2014-2018 4
... (Nasdaq: MYL ) today announced that Matrix ... a 71.2% controlling interest, has been selected by the ... the primary supplier of five antiretroviral (ARV) drugs used ... selected as the primary supplier of seven ARVs used ...
... Key Data are New Releases for the Company ... Technology Discoveries --DENVER, April 20 DXL625 ... commercial-stage antibodies in their ability to attach and ... to research conducted at InNexus Biotechnology Inc. (Toronto ...
... Psylin Neurosciences Inc., a joint venture biotechnology ... PsychoGenics, Inc. announced today that it has initiated ... the treatment of depression.(Logo: ... Amylin,s proprietary polypeptide hormone library, PHORMOL, was identified ...
Cached Biology Technology:Mylan's Matrix Selected by the Clinton HIV/AIDS Initiative and UNITAID as the Primary Supplier of Five Second-Line and Seven Pediatric HIV/AIDS Treatments 2Mylan's Matrix Selected by the Clinton HIV/AIDS Initiative and UNITAID as the Primary Supplier of Five Second-Line and Seven Pediatric HIV/AIDS Treatments 3AACR 100th Annual Meeting Highlights Superior Ability of InNexus' DXL-Modified Anti-CD20 Antibody to Attach and Kill NHL With Safety Comparable to Approved Antibody Therapies in Preclinical Studies 2AACR 100th Annual Meeting Highlights Superior Ability of InNexus' DXL-Modified Anti-CD20 Antibody to Attach and Kill NHL With Safety Comparable to Approved Antibody Therapies in Preclinical Studies 3Psylin Neurosciences Commences Development of Compound for Depression 2Psylin Neurosciences Commences Development of Compound for Depression 3