Navigation Links
Novel mechanism revealed for increasing recombinant protein yield in tobacco
Date:8/8/2009

Elastin-like polypeptides (ELPs) cause plants to store GM proteins in special 'protein bodies', insulating them from normal cellular degradation processes and increasing the overall protein yield. Researchers writing in the open access journal BMC Biology have visualised the mechanism by which the synthetic biopolymer increases the accumulation of recombinant proteins.

Rima Menassa worked with a team of researchers from Agriculture and Agri-Food Canada in London, Ontario, to develop and test the ELP tags by targeting an ELP-green fluorescent protein (GFP) fusion to various organelles in the leaves of the tobacco plant. Tobacco is well-suited as a production system for recombinant proteins but the mechanism by which ELP fusions increase production yields in transgenic tobacco leaves was previously unknown. Menassa said, "ELP was shown to almost double the yield of GFP to 11% of total soluble protein when hyperexpressed in the endoplasmic reticulum (ER)".

Based on their confocal and electron microscopy analyses, the researchers suggest that ELP fusions targeted to the ER induce the formation of novel mobile protein body-like structures in leaves, which appear similar in size and morphology to the prolamin-based protein bodies naturally found in plant seeds. These bodies may be responsible for ELP's positive effect on recombinant protein accumulation by excluding the heterologous protein from normal physiological turnover.

The researchers targeted their ELP fusions to the cytoplasm, chloroplasts, apoplast and ER in Nicotiana benthamiana tobacco plants. They found that the ER was the only intracellular compartment in which the ELP significantly enhanced recombinant protein accumulation. They conclude, "An ER-targeted ELP fusion approach provides an effective strategy for depositing large amounts of concentrated heterologous protein within the limited space of the cell".


'/>"/>

Contact: Graeme Baldwin
graeme.baldwin@biomedcentral.com
44-203-192-2165
BioMed Central
Source:Eurekalert

0

GOOD

Page: 1

Related biology news :

1. La Jolla Institute discovers novel tumor suppressor
2. Got zinc? New zinc research suggests novel therapeutic targets
3. Funxional successfully completes initial clinical trial of FX125L, an anti-inflammatory drug with a novel mechanism of action
4. Novel handheld device detects anthrax with outstanding accuracy and reliability
5. International collaboration by scientists culminates in novel ion channels database
6. UC Davis grant zeroes in on novel asthma diagnosis and treatment
7. Novel vaccine approach offers hope in fight against HIV
8. ExcellGene and Khner achieved milestone in development of novel 250 Liter mammalian cell culture bioreactors
9. Study finds novel genetic risk factors for kidney disease
10. Novel antibody prevents infection by hepatitis C virus
11. IVCC and Syngenta start project for novel insecticidal active ingredient for malaria control
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/17/2013)... and Sustainable Global Logistics Operations) is aiming to cut ... of intermodal loading systems and the boosting of intelligent ... organisations with experience in the logistics sector and in ... and a duration of three and a half years. ... semantic components and ontologies shared by the users, objects ...
(Date:5/17/2013)... Bethesda, MD The Federation of American Societies for ... of National Institutes of Health (NIH) funding trends highlighting ... to support critical research. The FASEB analysis follows a ... Francis Collins, MD, PhD, who testified that the $1.6 ... to sequestration is "having a substantial impact on the ...
(Date:5/16/2013)... 2013) Illustrating a commitment to the support ... Association (AGA) Research Foundation has announced the inaugural ... Award recipients. Supported by the National Institute of ... new award helps underrepresented minority students to further ... research. , "By establishing this new award, AGA ...
Breaking Biology News(10 mins):New logistics services that will cut energy consumption and CO2 emissions 2Underrepresented minority students receive fellowships in digestive disease and nutrition research 2
... in partnership with Oak Ridge National Laboratory (ORNL) will ... the National Science Foundation (NSF) has deemed the "most ... The two will team up to participate ... continental-scale ecological observatory, to answer questions that deal with ...
... Mass. A six-year campaign to control invasive winter ... Elkinton of the University of Massachusetts Amherst now has ... been established and is attacking the pest at four ... beginning of the end for the decade-long defoliation of ...
... (Santa Barbara, Calif.) Sensors made from custom DNA molecules ... quality of stem cells, according to an international team of ... University of Rome Tor Vergata. The new nanosensors can ... which serve as the master control switches of life. The ...
Cached Biology News:University of Tennessee chosen to join national network to study climate change 2UMass Amherst entomologists begin to control winter moth infestation in eastern Massachusetts 2Nanosensors made from DNA may light path to new cancer tests and drugs 2Nanosensors made from DNA may light path to new cancer tests and drugs 3
(Date:5/17/2013)... May 17, 2013  Cellular Biomedicine Group (OTCQB: ... enrollment of the total projected enrollment required for ... and preliminary efficacy of the medical technology haMPC ... Osteoarthritis (KOA). To date the trial has had ... The Phase I open label clinical research trial ...
(Date:5/17/2013)... 17, 2013 /PRNewswire-iReach/ -- Aridis is pleased to ... Switzerland -based Kenta Biotech to ... antibody (mAb) products, and technologies. This asset portfolio ... treatment of infections by common pathogens including drug ... Pseudomonas aeruginosa , Acinetobacter baumannii ...
(Date:5/17/2013)... Men’s skin is biologically different than women’s skin,( says ... been known to react more aggressively when hormones change, and ... puberty, other acne causing factors like P.acnes bacteria and excess ... with unsightly acne scars. Adult acne treatment, Probiotic Action shares ... how to reduce and prevent acne scarring. , In order ...
(Date:5/17/2013)... MD (PRWEB) May 17, 2013 ... of ezColony version 4.1, a versatile refinement to ... premier software solution, improves its advanced methodical management ... facilities with ezColony 4.1. The network-based platform provides ... conduct effective research. While continuing to emphasize streamlined ...
Breaking Biology Technology:Cellular Biomedicine Group Achieves 50% Enrollment Milestone in Phase I Clinical Trial for Knee Osteoarthritis 2Aridis Pharmaceuticals Announces Acquisition of Monoclonal Antibody Products and Technologies From Kenta Biotech 2Aridis Pharmaceuticals Announces Acquisition of Monoclonal Antibody Products and Technologies From Kenta Biotech 3Adult Acne Treatment, Probiotic Action Shares New Insight on Why Men’s Skin Scars from Acne and How to Prevent It 2RURO Introduces ezColony 4.1 – The Versatile Transgenic Animal Colony Management Software 2
... ... and Ralph M. Lerner named Senior Vice President of Commercial Development , ... Marlborough, Mass. (Vocus) October 23, ... technology turns biomass into cellulosic ethanol, has welcomed Kevin F. McLaughlin and Ralph M. ...
... Thompson, President and CEO of Oncolytics Biotech Inc. (TSX: ... overview of the Company at the 8th Annual BIO ... 3:00 p.m. PT. The conference will be held at ... and 29, 2009. , A live audio link to ...
... Oct. 23 /PRNewswire-FirstCall/ - AEterna Zentaris Inc. (NASDAQ: ... global biopharmaceutical company focused on endocrine therapy and ... previously announced registered direct offering of US$5.5 million ... share purchase warrants to certain institutional investors. AEterna ...
Cached Biology Technology:Cellulosic Biofuels Leader Qteros Adds Key Positions 2Cellulosic Biofuels Leader Qteros Adds Key Positions 3Media Advisory - Oncolytics Biotech Inc. to Present at BIO Investor Forum 2009 2AEterna Zentaris Receives US$5.5 Million from Institutional Investors 2AEterna Zentaris Receives US$5.5 Million from Institutional Investors 3
Peptide-affinity Purified Polyclonal Antibody to Prolactin Releasing Hormone Receptor (GPR10)...
Peptide-affinity Purified Polyclonal Antibody to Sphingolipid Receptor Edg8/S1P5...
Peptide-affinity Purified Polyclonal Antibody to MAML1 / Mastermind Peptide with sequence VLPTCPMAEFALPR, from N Terminus of the protein sequence according to NP_055572....
DNA sequencing - plasmids or PCR fragments, 3-7 working days service....
Biology Products: