Navigation Links
Novel mechanism revealed for increasing recombinant protein yield in tobacco
Date:8/8/2009

Elastin-like polypeptides (ELPs) cause plants to store GM proteins in special 'protein bodies', insulating them from normal cellular degradation processes and increasing the overall protein yield. Researchers writing in the open access journal BMC Biology have visualised the mechanism by which the synthetic biopolymer increases the accumulation of recombinant proteins.

Rima Menassa worked with a team of researchers from Agriculture and Agri-Food Canada in London, Ontario, to develop and test the ELP tags by targeting an ELP-green fluorescent protein (GFP) fusion to various organelles in the leaves of the tobacco plant. Tobacco is well-suited as a production system for recombinant proteins but the mechanism by which ELP fusions increase production yields in transgenic tobacco leaves was previously unknown. Menassa said, "ELP was shown to almost double the yield of GFP to 11% of total soluble protein when hyperexpressed in the endoplasmic reticulum (ER)".

Based on their confocal and electron microscopy analyses, the researchers suggest that ELP fusions targeted to the ER induce the formation of novel mobile protein body-like structures in leaves, which appear similar in size and morphology to the prolamin-based protein bodies naturally found in plant seeds. These bodies may be responsible for ELP's positive effect on recombinant protein accumulation by excluding the heterologous protein from normal physiological turnover.

The researchers targeted their ELP fusions to the cytoplasm, chloroplasts, apoplast and ER in Nicotiana benthamiana tobacco plants. They found that the ER was the only intracellular compartment in which the ELP significantly enhanced recombinant protein accumulation. They conclude, "An ER-targeted ELP fusion approach provides an effective strategy for depositing large amounts of concentrated heterologous protein within the limited space of the cell".


'/>"/>

Contact: Graeme Baldwin
graeme.baldwin@biomedcentral.com
44-203-192-2165
BioMed Central
Source:Eurekalert

Page: 1

Related biology news :

1. La Jolla Institute discovers novel tumor suppressor
2. Got zinc? New zinc research suggests novel therapeutic targets
3. Funxional successfully completes initial clinical trial of FX125L, an anti-inflammatory drug with a novel mechanism of action
4. Novel handheld device detects anthrax with outstanding accuracy and reliability
5. International collaboration by scientists culminates in novel ion channels database
6. UC Davis grant zeroes in on novel asthma diagnosis and treatment
7. Novel vaccine approach offers hope in fight against HIV
8. ExcellGene and Khner achieved milestone in development of novel 250 Liter mammalian cell culture bioreactors
9. Study finds novel genetic risk factors for kidney disease
10. Novel antibody prevents infection by hepatitis C virus
11. IVCC and Syngenta start project for novel insecticidal active ingredient for malaria control
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/31/2016)... RATON, Florida , March 31, 2016 ... LEGX ) ("LegacyXChange" or the "Company") ... for potential users of its soon to be launched ... video ( https://www.youtube.com/channel/UCyTLBzmZogV1y2D6bDkBX5g ) will also provide ... the use of DNA technology to an industry that ...
(Date:3/22/2016)... , PROVO and ... Newborn Screening Ontario (NSO), which operates the highest sample ... molecular testing, and Tute Genomics and UNIConnect, leaders in ... respectively, today announced the launch of a project to ... (NGS) testing panel. NSO has been ...
(Date:3/15/2016)... , March 15, 2016 Yissum ... , the technology-transfer company of the Hebrew University, announced ... of remote sensing technology of various human biological indicators. ... raising $2.0 million from private investors. ... based on the detection of electromagnetic emissions from sweat ...
Breaking Biology News(10 mins):
(Date:5/25/2016)... ... May 25, 2016 , ... Scientists at the University of Athens say they ... may be hampering the research that could lead to one good one. Surviving Mesothelioma ... it now. , The team evaluated 98 mesothelioma patients who got ...
(Date:5/24/2016)... (PRWEB) , ... May 24, 2016 , ... ... attacks, diabetes, and traumatic injuries, will be accelerated by research at Worcester Polytechnic ... into engines of wound healing and tissue regeneration. , The novel method, developed ...
(Date:5/23/2016)... ... May 23, 2016 , ... The need for blood donations in South Texas and ... the South Texas Blood & Tissue Center, blood donations are on the decline. In fact, ... they are down 21 percent in South Texas in the last four years alone. , ...
(Date:5/23/2016)... LONDON , May 23, 2016 - ... by 40% - Frontage Implement a Single Platform to ... Compliance and Traceability Within the Bioanalytical lab Frontage Laboratories, ... the United States and China , ... its laboratory facilities. In addition to serving as the global electronic ...
Breaking Biology Technology: