Navigation Links
Novel mechanism revealed for increasing recombinant protein yield in tobacco
Date:8/8/2009

Elastin-like polypeptides (ELPs) cause plants to store GM proteins in special 'protein bodies', insulating them from normal cellular degradation processes and increasing the overall protein yield. Researchers writing in the open access journal BMC Biology have visualised the mechanism by which the synthetic biopolymer increases the accumulation of recombinant proteins.

Rima Menassa worked with a team of researchers from Agriculture and Agri-Food Canada in London, Ontario, to develop and test the ELP tags by targeting an ELP-green fluorescent protein (GFP) fusion to various organelles in the leaves of the tobacco plant. Tobacco is well-suited as a production system for recombinant proteins but the mechanism by which ELP fusions increase production yields in transgenic tobacco leaves was previously unknown. Menassa said, "ELP was shown to almost double the yield of GFP to 11% of total soluble protein when hyperexpressed in the endoplasmic reticulum (ER)".

Based on their confocal and electron microscopy analyses, the researchers suggest that ELP fusions targeted to the ER induce the formation of novel mobile protein body-like structures in leaves, which appear similar in size and morphology to the prolamin-based protein bodies naturally found in plant seeds. These bodies may be responsible for ELP's positive effect on recombinant protein accumulation by excluding the heterologous protein from normal physiological turnover.

The researchers targeted their ELP fusions to the cytoplasm, chloroplasts, apoplast and ER in Nicotiana benthamiana tobacco plants. They found that the ER was the only intracellular compartment in which the ELP significantly enhanced recombinant protein accumulation. They conclude, "An ER-targeted ELP fusion approach provides an effective strategy for depositing large amounts of concentrated heterologous protein within the limited space of the cell".


'/>"/>

Contact: Graeme Baldwin
graeme.baldwin@biomedcentral.com
44-203-192-2165
BioMed Central
Source:Eurekalert

Page: 1

Related biology news :

1. La Jolla Institute discovers novel tumor suppressor
2. Got zinc? New zinc research suggests novel therapeutic targets
3. Funxional successfully completes initial clinical trial of FX125L, an anti-inflammatory drug with a novel mechanism of action
4. Novel handheld device detects anthrax with outstanding accuracy and reliability
5. International collaboration by scientists culminates in novel ion channels database
6. UC Davis grant zeroes in on novel asthma diagnosis and treatment
7. Novel vaccine approach offers hope in fight against HIV
8. ExcellGene and Khner achieved milestone in development of novel 250 Liter mammalian cell culture bioreactors
9. Study finds novel genetic risk factors for kidney disease
10. Novel antibody prevents infection by hepatitis C virus
11. IVCC and Syngenta start project for novel insecticidal active ingredient for malaria control
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/2/2014)... legs and their propensity for eating anything, including each ... now research from North Carolina State University finds that ... the eastern United States. , "The good news is ... of threat to humans," says Dr. Mary Jane Epps, ... of a paper about the research. , The research ...
(Date:9/2/2014)... hot oven, you rapidly pull your hand away. Although ... and responding to such painful stimuli, they are still ... made a surprising discovery about the role of a ... built a structural model of the molecule. These discoveries, ... help direct new strategies to treat pain in people. ...
(Date:9/1/2014)... Is television making us fat? An increasing amount of ... food consumption and a more sedentary lifestyle. Now, a ... TV is alike. Some TV programs might lead people ... find that if you,re watching an action movie while ... Aner Tal, Ph.D. lead author on the new article ...
Breaking Biology News(10 mins):Researchers find Asian camel crickets now common in US homes 2Surprising new role for calcium in sensing pain 2Surprising new role for calcium in sensing pain 3Can action movies make you fat? 2
... have determined the molecular structure of a viral protein, ... virus 5 is part of a family of viruses ... to cold-like illness and is responsible for many hospitalizations ... Jan. 5 by the journal Nature. , Details of ...
... the detailed structure of an essential piece of the ... of human cancers. Understanding the physical shape of the ... it acts to immortalize cells ?and should help scientists ... lack of detailed structural information about the enzyme has ...
... from The Walter and Eliza Hall Institute, using a ... drives the formation of all breast tissue. This discovery ... tissue develops. The identification of the breast stem cell ... cancer develops and how rogue cells evade current therapies. ...
Cached Biology News:Scientists identify molecular structure of key viral protein 2Scientists identify molecular structure of key viral protein 3Scientists identify molecular structure of key viral protein 4Report lists top 20 most-vulnerable African carnivores 2Report lists top 20 most-vulnerable African carnivores 3Report lists top 20 most-vulnerable African carnivores 4Faults in newly discovered breast stem cells may lead to tumours 2
(Date:9/2/2014)... billionth of a meter in size, are around us ... human health, as in some innovative early cancer treatments, ... air pollution, traffic emissions, cosmetics, sunscreen and electronics. , ... Louis, led by Lan Yang, PhD, the Das Family ... and their collaborators at Tsinghua University in China have ...
(Date:9/2/2014)... 2, 2014 Persistence Market Research ... in Pharmaceutical, Biopharma and Life Sciences: North ... the global membrane technology market for pharmaceutical, biopharma and life sciences ... is expected to grow at a CAGR of 9.1% ... of USD 10,886.0 million in 2019. Browse ...
(Date:9/2/2014)... PA (PRWEB) September 02, 2014 ... a new highly efficient, cost effective approach to ... Patent No. 8,820,265 by the U.S. Patent and ... to Opertech. , “Opertech’s taste evaluation ... the first automated high throughput system for rapid ...
(Date:9/2/2014)... Sept. 2, 2014  Synthetic Biologics, Inc. (NYSE ... and drug candidates targeting specific pathogens that cause ... from its final preclinical toxicology study of SYN-004. ... this bridging study was required to move the ... prevent the devastating effects of Clostridium difficile ...
Breaking Biology Technology:Engineers develop new sensor to detect tiny individual nanoparticles 2Engineers develop new sensor to detect tiny individual nanoparticles 3Global Membrane Technology Market Will Reach $10,886.0 Million in 2019: Persistence Market Research 2Global Membrane Technology Market Will Reach $10,886.0 Million in 2019: Persistence Market Research 3Global Membrane Technology Market Will Reach $10,886.0 Million in 2019: Persistence Market Research 4Global Membrane Technology Market Will Reach $10,886.0 Million in 2019: Persistence Market Research 5Opertech Bio Receives U.S. Patent for High-Throughput Taste Evaluation Technology 2Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 2Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 3Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 4Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 5
... KANNAPOLIS, N.C. , May 26 The Immune ... Institute (DHMRI) today announced a collaboration to establish the Center ... H. Murdock Research Institute on the North Carolina Research Campus ... accelerate the discovery and development of new breakthrough treatments for ...
... 26 /PRNewswire-Asia-FirstCall/ -- NeoStem, Inc.,(NYSE Amex: NBS ) ("NeoStem" or the "Company"), ... the Company,s Chief Executive Officer, Robin Smith , M.D., will,ring the opening bell ... 9:30 a.m. ET on Thursday, May 27, 2010 . , ... "We are honored to be back at ...
... CHICAGO , May 26 Advanced ... ADLS), a biopharmaceutical company engaged in the discovery, development and ... oncology and respiratory diseases, today announced positive results from ... the efficacy of Restanza™ (cethromycin), its novel oral antibiotic, against ...
Cached Biology Technology:The Immune Tolerance Institute and David H. Murdock Research Institute Announce a Partnership Establishing a State of the Art Human Immune Monitoring Laboratory for Biomarker Discovery and Development 2The Immune Tolerance Institute and David H. Murdock Research Institute Announce a Partnership Establishing a State of the Art Human Immune Monitoring Laboratory for Biomarker Discovery and Development 3NeoStem to Ring Opening Bell at New York Stock Exchange on May 27, 2010 2NeoStem to Ring Opening Bell at New York Stock Exchange on May 27, 2010 3NeoStem to Ring Opening Bell at New York Stock Exchange on May 27, 2010 4Advanced Life Sciences Announces Restanza(TM) Shows Efficacy Against Malaria 2Advanced Life Sciences Announces Restanza(TM) Shows Efficacy Against Malaria 3Advanced Life Sciences Announces Restanza(TM) Shows Efficacy Against Malaria 4Advanced Life Sciences Announces Restanza(TM) Shows Efficacy Against Malaria 5