BOSTON (February 23, 2012, 5:00 pm ET) Researchers have combined bioactive peptides to successfully stimulate wound healing. The in vitro and in vivo study, published today in PLoS ONE, demonstrates that the combination of two peptides stimulates the growth of blood vessels and promotes re-growth of tissue. Further development of these peptides could lead to a new treatment for chronic and acute wounds.
The team tested a newly-created peptide, UN3, in pre-clinical models aimed to simulate impaired wound healing as is seen in patients with peripheral vascular diseases or uncontrolled diabetes. The peptide led to a 50% increase in blood vessel wall development, a 250% increase in growth of blood vessels, and a 300% increase in cell migration in response to the injury.
"Using double-blinded in vivo experiments, we then applied the wound-healing peptide UN3 with a peptide created during a previous study, named comb1. We found that, together, the two out-performed all control groups, including the only FDA-approved growth factor-containing drug for treating diabetic wounds, becaplermin," said first author, Tatiana Demidova-Rice, PhD, now a graduate of the cell, molecular and developmental biology program at the Sackler School of Graduate Biomedical Sciences.
In December 2010, Herman and Demidova-Rice identified several peptides from a Clostridium histolyticum collagenase treatment of bio-synthesized extracellular matrix. The investigators then went on to cull key peptides and, from these, they created the peptide, comb1, possessing several strategic features, including its ability to stimulate angiogenesis by increasing blood vessel growth by 200% in vitro.
The second wound-healing peptide, UN3, identified in the current study, was created and modified from two naturally occurring peptides that are normally present in trace amounts and found in human platelet-rich plasma.
"The confirmation that these
|Contact: Siobhan E. Gallagher|
Tufts University, Health Sciences Campus