Navigation Links
Novel approach to gene regulation can activate multiple genes simultaneously
Date:8/27/2013

CAMBRIDGE, Mass. (August 27, 2013) By creating a powerful new gene regulation system called CRISPR-on, Whitehead Institute researchers now have the ability to increase the expression of multiple genes simultaneously and precisely manipulate each gene's expression level. The system is effective in both mouse and human cells as well as in mouse embryos.

"CRISPR-on is a tool that will be very useful for studying many biological processes, particularly for studying gene functions and gene networks," says Whitehead Founding Member Rudolf Jaenisch. "In contrast to RNA interference, which is commonly used to inactivate gene activity, the CRISPR-on system allows activation of cellular genes. The technology substantially expands our ability to change gene expression in cultured cells and animals.."

The system, called CRISPR-on, is a modified version of CRISPR/Cas (for "clustered regularly interspaced short palindromic repeat/CRISPR associated"), which taps into a bacterial defense system against viral intruders. CRISPR/Cas relies on an enzyme, Cas9, which cuts DNA at locations specified by single guide RNAs (sgRNAs). For CRISPR-on, the Whitehead team modified the Cas9 enzyme by eliminating its ability to cleave DNA and adding a transcription activation domain. The resulting enzyme can increase gene expression without permanently changing the DNA.

The new system is described this week in the journal Cell Research.

CRISPR-on's ability to activate only the desired genes at varying levels could be used to help scientists improve our understanding of transcription network underlying a variety of diseases and potentially find new ways to treat them.

"Many diseases, especially complex diseases, involve multiple genes, and this system could be used therapeutically to target and activate multiple genes together and rescue these disease phenotypes," says Albert Cheng, a graduate student in the Jaenisch lab and co-author of the Cell Research paper. "Or we could use it to study the gene networks in diseases and get a better understanding of how those diseases work."

So far, the researchers have used CRISPR-on to activate up to three native genes concurrently in human cells.

"I think we need to do more work to see if there are any limitations to the number of genes CRISPR-on can activate at a time," says Haoyi Wang, a co-author and postdoctoral researcher in the Jaenisch lab. "We'd like to see if we can get data on activating 10 or more genes, to see if there is an upper limit to what this system can do."


'/>"/>

Contact: Nicole Rura
rura@wi.mit.edu
617-258-6851
Whitehead Institute for Biomedical Research
Source:Eurekalert

Related biology news :

1. Cancer scientists discover novel way gene controls stem cell self-renewal
2. Novel Chinese herbal medicine JSK improves spinal cord injury outcomes in rats
3. Study finds novel worm community affecting methane release in ocean
4. UT Southwestern researchers identify novel mechanism that helps stomach bug cause illness
5. Novel chemistry for new class of antibiotic
6. Novel enzyme from tiny gribble could prove a boon for biofuels research
7. Novel biomarkers improve diagnosis in rheumatoid arthritis
8. Patent awarded today to NJIT for novel use of water jets to create high tensile strength alloy parts
9. U of M researchers find novel gene correction model for epidermolysis bullosa
10. University of Maryland School of Medicine researchers find potential novel treatment for influenza
11. Novel screening tests for liver cancer
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/22/2016)... , June 22, 2016 On Monday, ... call to industry to share solutions for the Biometric ... U.S. Customs and Border Protection (CBP), explains that CBP ... are departing the United States , ... and to defeat imposters. Logo - ...
(Date:6/9/2016)... ISTANBUL , June 9, 2016  Perkotek an innovation leader in attendance control ... to seamlessly log work hours, for employers to make sure the right employees are ... Logo - http://photos.prnewswire.com/prnh/20160609/377486LOGO ... ... ...
(Date:6/2/2016)... NEW YORK , June 2, 2016   The ... (Weather), is announcing Watson Ads, an industry-first capability in which ... advertising, by being able to ask questions via voice or ... Marketers have long ... with the consumer, that can be personal, relevant and valuable; ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... 2016 Andrew D ... http://doi.org/10.17925/OHR.2016.12.01.22 Published recently in ... from touchONCOLOGY, Andrew D Zelenetz , discusses ... care is placing an increasing burden on healthcare ... therapies. With the patents on many biologics expiring, ...
(Date:6/23/2016)... ... June 23, 2016 , ... ... the Pennsylvania Convention Center and will showcase its product’s latest features from June ... be presenting a scientific poster on Disrupting Clinical Trials in The Cloud during ...
(Date:6/23/2016)... ... 2016 , ... Velocity Products, a division of Morris Group, ... exclusively for Okuma CNC machining centers at The International Manufacturing Technology Show, IMTS, ... companies with expertise in toolholding, cutting tools, machining dynamics and distribution, Velocity SMART ...
(Date:6/23/2016)... ReportsnReports.com adds 2016 global ... pharmaceuticals section with historic and forecast data along ... Complete report on the Cell Culture ... companies and supported with 261 tables and figures ... The Global Cell Culture Media Industry ...
Breaking Biology Technology: