Navigation Links
Notre Dame scientists develop largest developmental proteomic data set for any animal
Date:3/27/2014

Now that the human genome is sequenced, University of Notre Dame researchers are focusing on the study of the proteome, which is the protein content of an organism, tissue or cell. Bioanalytical chemist Norman Dovichi and molecular biologist Paul Huber have successfully tracked the changing patterns of protein expression during early development of Xenopus laevis, or African clawed frog, embryos. They have developed the largest data set on developmental proteomics for any organism, and have included the single-cell zygote.

Their research has uncovered an unexpected amount of discordance between the levels of messenger RNA (mRNA) and its corresponding protein. Their findings are published in Scientific Reports in an article titled, "Quantitative proteomics of Xenopus laevis embryos: expression kinetics of nearly 4000 proteins during early development."

The Notre Dame team based in the Department of Chemistry and Biochemistry in the College of Science has identified and measured the levels of about 4,000 proteins, which exhibited patterns of expression that reflect key events during early Xenopus development. For example, the appearance of organ- and tissue-specific proteins, such as those found exclusively in cardiac muscle cells, accurately reflects imminent anatomical changes taking place in the embryo. The research could lead to insight into congenital birth defects that result from the misregulation of gene expression.

The research also contradicted a widely held assumption that the levels of mRNA, which encodes proteins, would be directly related to protein levels. While that was true in most cases, there were a surprisingly high number of exceptions, demonstrating that the amounts of a particular protein can be controlled by multiple mechanisms.

Because development takes place in well-defined stages outside the mother, Xenopus is a favored model. Embryogenesis can be easily monitored in real time; fate maps for organ development have been determined and major regulators of these processes have been identified and characterized, providing an abundance of tissue- and organ-specific markers to track embryo formation. Additionally, embryos develop rapidly, achieving a nearly fully developed nervous system within four days. "It's easy to manipulate the embryos to mimic certain disease states, making Xenopus extremely valuable to biologists," Huber said.

"The collaborative, ground-breaking work of Norm Dovichi, Paul Huber and their team is crucial to helping us understand the complexity of life. We are proud of this important milestone," said Greg Crawford, dean of the College of Science at the University of Notre Dame.


'/>"/>

Contact: Norm Dovichi
ndovichi@nd.edu
574-631-2778
University of Notre Dame
Source:Eurekalert

Related biology news :

1. Notre Dame chemists discover new class of antibiotics
2. Notre Dame paper sheds light on genetic and physiological basis for metabolic diseases
3. Notre Dame researchers develop system that uses a big data approach to personalized healthcare
4. Notre Dame study finds Asian carp DNA not widespread in the Great Lakes
5. Notre Dame researcher is studying role small dams play in pollution control
6. Notre Dame researchers are using new technologies to combat invasive species
7. Notre Dame research may have important implications for combating diabetes
8. Cargill expands support of Notre Dame Haiti Program
9. Notre Dame research reveals migrating Great Lakes salmon carry contaminants upstream
10. Notre Dame research could improve sustainability and cost effectiveness of wastewater treatment
11. Notre Dame research could provide new insights into tuberculosis and other diseases
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/9/2016)... Paris Police Prefecture ... solution to ensure the safety of people and operations in ... major tournament Teleste, an international technology group specialised ... today that its video security solution will be utilised by ... public safety across the country. The system roll-out is scheduled ...
(Date:6/3/2016)... Das DOTM (Department ... hat ein 44 Millionen $-Projekt ... einschließlich Personalisierung, Registrierung und IT-Infrastruktur, an Decatur ... Implementierung von Identitätsmanagementlösungen. Zahlreiche renommierte internationale Anbieter ... aber Decatur wurde als konformste und innovativste ...
(Date:6/2/2016)... June 2, 2016   The Weather Company , an ... Ads, an industry-first capability in which consumers will be able ... to ask questions via voice or text and receive relevant ... Marketers have long sought an advertising solution ... can be personal, relevant and valuable; and can scale across ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... on a range of subjects including policies, debt and investment ... Speaking at a lecture to the Canadian Economics ... the country,s inflation target, which is set by both the ... "In certain areas there needs to be frequent ... not sit down and address strategy together?" He ...
(Date:6/23/2016)... ... June 23, 2016 , ... ... of its second eBook, “Clinical Trials Patient Recruitment and Retention Tips.” Partnering with ... in this eBook by providing practical tips, tools, and strategies for clinical researchers. ...
(Date:6/23/2016)... Md. , June 23, 2016 A person ... from the crime scene to track the criminal down. ... the U.S. Food and Drug Administration (FDA) uses DNA evidence ... Sound far-fetched? It,s not. The FDA ... sequencing to support investigations of foodborne illnesses. Put as simply ...
(Date:6/23/2016)... June, 23, 2016  The Biodesign Challenge (BDC), a ... ways to harness living systems and biotechnology, announced its ... in New York City . ... students, showcased projects at MoMA,s Celeste Bartos Theater during ... , MoMA,s senior curator of architecture and design, and ...
Breaking Biology Technology: