Navigation Links
Notre Dame research could provide new insights into tuberculosis and other diseases
Date:9/18/2012

Researchers Patricia A. Champion and Matthew Champion from the University of Notre Dame's Eck Institute for Global Health have developed a method to directly detect bacterial protein secretion, which could provide new insights into a variety of diseases including tuberculosis.

The Champions point out that bacteria use a variety of secretion systems to transport proteins beyond their cell membrane in order to interact with their environment. For bacterial pathogens like TB these systems transport bacterial proteins that promote interaction with host cells, leading to virulent disease.

Previously, researchers have relied on methods that have fused enzymes or fluorescent markers to bacterial proteins to identify bacterial genes that are used to export bacterial proteins into host cells. However, these methods can't be used in the analysis of all bacterial secretion systems, which has limited understanding of the mechanisms that bacteria use to interact with host cells.

The Champions developed a modified form of bacterial proteomics using a MALDI-TOF mass spectrometer, which directly detects the proteins from whole-colonies by ionizing them with a laser. This research revealed that the method was able to specifically monitor a specialized form protein secretion, which is a major virulence determinant in both mycobacterial pathogens, such as TB, and Gram-positive pathogens, such as Bacillus and Staphylococcus species.

The Champions demonstrated that this new method is applicable to the study of other bacterial protein export systems that could not be effectively studied under previous methods. Their method could also help in the identification of compounds that can inhibit bacterial protein secretion.

The method's importance can be seen in the fact that there are approximately 2 million fatal TB cases a year, mostly in the developing world. Also, antibiotic resistant strains of TB are appearing increasingly.

The Champions' research findings appeared in the Journal Molecular and Cellular Proteomics. The research was funded by the National Institutes of Health and Notre Dame's Center for Rare and Neglected Diseases and capitalization funds from Notre Dame.


'/>"/>

Contact: Patricia A. Champion
Patricia.A.Champion.7@nd.edu
574-631-8375
University of Notre Dame
Source:Eurekalert

Related biology news :

1. Notre Dame researcher is shedding light on how jaws evolve
2. Notre Dame establishes professorships in adult stem cell research
3. Notre Dame researchers using novel method to combat malaria drug resistance
4. New paper by Notre Dame researchers describes method for cleaning up nuclear waste
5. RUB researchers clarify catalysis mechanism of cell growth protein Ras
6. UCLA Receives $46 Thousand Check for Groundbreaking Research on Debilitating Pregnancy Disease
7. Team including UC Riverside entomologist honored for research leading to healthier potato chips
8. Einstein researcher receives $10.8 million grant to study toxic blood reactions caused by hemoglobin
9. Military vision research symposium focuses on visual fallout from war
10. Could bioscience research bring more Olympic and Paralympic medals
11. Researchers find our inner reptile hearts
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/14/2016)... Israel , April 14, 2016 ... Authentication and Malware Detection, today announced the appointment of ... assumed the new role. Goldwerger,s leadership appointment ... on the heels of the deployment of its platform ... BioCatch,s behavioral biometric technology, which discerns unique cognitive and ...
(Date:3/31/2016)... , March 31, 2016 ... ) ("LegacyXChange" or the "Company") LegacyXChange is ... users of its soon to be launched online site ... https://www.youtube.com/channel/UCyTLBzmZogV1y2D6bDkBX5g ) will also provide potential shareholders ... of DNA technology to an industry that is notorious ...
(Date:3/29/2016)... Florida , March 29, 2016 ... the "Company") LegacyXChange "LEGX" and SelectaDNA/CSI Protect are pleased ... in ink used in a variety of writing instruments, ... Buyers of originally created collectibles from athletes on LegacyXChange ... forensic analysis of the DNA. Bill ...
Breaking Biology News(10 mins):
(Date:5/4/2016)... , ... May 04, 2016 , ... PBI-Gordon Corporation is ... Agricultural Sales. , Doug began his career at PBI-Gordon in February 1988, after ... a wide variety of roles, ranging from customer service to national product manager, to ...
(Date:5/3/2016)... ... 03, 2016 , ... Morf Media Inc ., developer ... on mobile devices, today released a new interactive Food and Drug Administration ... course is essential for owners or operators of places of business that are ...
(Date:5/3/2016)... CO (PRWEB) , ... May 03, 2016 , ... ... , announced the addition of Dr. Nancy Gillett to its Board of Directors. ... position, she served as Corporate Executive Vice President and Chief Scientific Officer. A ...
(Date:5/3/2016)... LONDON , May 3, 2016 ... Report Assessing Developers and Producers of Those Competitor Biologics  ... Guide to Companies, Activities and Prospects ,  ... drug companies? And what are their sales potentials? ... There you see results, trends, opportunities and revenue forecasting. ...
Breaking Biology Technology: