Navigation Links
Notre Dame research could provide new insights into tuberculosis and other diseases
Date:9/18/2012

Researchers Patricia A. Champion and Matthew Champion from the University of Notre Dame's Eck Institute for Global Health have developed a method to directly detect bacterial protein secretion, which could provide new insights into a variety of diseases including tuberculosis.

The Champions point out that bacteria use a variety of secretion systems to transport proteins beyond their cell membrane in order to interact with their environment. For bacterial pathogens like TB these systems transport bacterial proteins that promote interaction with host cells, leading to virulent disease.

Previously, researchers have relied on methods that have fused enzymes or fluorescent markers to bacterial proteins to identify bacterial genes that are used to export bacterial proteins into host cells. However, these methods can't be used in the analysis of all bacterial secretion systems, which has limited understanding of the mechanisms that bacteria use to interact with host cells.

The Champions developed a modified form of bacterial proteomics using a MALDI-TOF mass spectrometer, which directly detects the proteins from whole-colonies by ionizing them with a laser. This research revealed that the method was able to specifically monitor a specialized form protein secretion, which is a major virulence determinant in both mycobacterial pathogens, such as TB, and Gram-positive pathogens, such as Bacillus and Staphylococcus species.

The Champions demonstrated that this new method is applicable to the study of other bacterial protein export systems that could not be effectively studied under previous methods. Their method could also help in the identification of compounds that can inhibit bacterial protein secretion.

The method's importance can be seen in the fact that there are approximately 2 million fatal TB cases a year, mostly in the developing world. Also, antibiotic resistant strains of TB are appearing increasingly.

The Champions' research findings appeared in the Journal Molecular and Cellular Proteomics. The research was funded by the National Institutes of Health and Notre Dame's Center for Rare and Neglected Diseases and capitalization funds from Notre Dame.


'/>"/>

Contact: Patricia A. Champion
Patricia.A.Champion.7@nd.edu
574-631-8375
University of Notre Dame
Source:Eurekalert

Related biology news :

1. Notre Dame researcher is shedding light on how jaws evolve
2. Notre Dame establishes professorships in adult stem cell research
3. Notre Dame researchers using novel method to combat malaria drug resistance
4. New paper by Notre Dame researchers describes method for cleaning up nuclear waste
5. RUB researchers clarify catalysis mechanism of cell growth protein Ras
6. UCLA Receives $46 Thousand Check for Groundbreaking Research on Debilitating Pregnancy Disease
7. Team including UC Riverside entomologist honored for research leading to healthier potato chips
8. Einstein researcher receives $10.8 million grant to study toxic blood reactions caused by hemoglobin
9. Military vision research symposium focuses on visual fallout from war
10. Could bioscience research bring more Olympic and Paralympic medals
11. Researchers find our inner reptile hearts
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/4/2016)... The field of Human Microbiome research ... popular hubs of the biotechnology industry. While the ... of human microbiota, have garnered a lot of ... space has literally exploded in terms of both ... focuses on biomedical aspects of research, development, and ...
(Date:2/3/2016)... DUBLIN , Feb. 3, 2016 /PRNewswire/ ... announced the addition of the "Emotion ... NLP, Machine Learning, and Others), Software Tools ... Application Areas, End Users,and Regions - Global ... offering. --> http://www.researchandmarkets.com/research/d8zjcd/emotion_detection ) ...
(Date:2/2/2016)... 2, 2016 Technology Enhancements Accelerate Growth of X-ray ... the digital and computed radiography markets in ... Indonesia (TIM). It provides an ... as well as regional market drivers and restraints. The ... penetration and market attractiveness, both for digital and computed ...
Breaking Biology News(10 mins):
(Date:2/5/2016)... , Feb. 5, 2016 Australian-US drug discovery ... announced today the appointment of a new Chairman, Mr ... Carmine , effective immediately. James Garner , ... Executive Director and former Acting CEO, Mr Iain Ross ... Non-Executive Director. --> James Garner , has also ...
(Date:2/4/2016)... , Feb. 4, 2016  Sangamo BioSciences, Inc. ... editing, announced today that Edward Lanphier , Sangamo,s ... on the progress of Sangamo,s ZFP Therapeutic ® ... strategy at 2:40 pm ET on Thursday, February 11, ... Global Healthcare Conference. The conference is being held in ...
(Date:2/4/2016)... , Feb. 4, 2016 Beike Biotechnology, the ... medical institutions attended a ceremony in late 2015 to ... cell therapy in 2016. --> ... Translation Platform for Personalized Cell Therapy" was hosted by ... Production Center, both subsidiaries of Beike Biotechnology Co., Ltd. ...
(Date:2/4/2016)... --> --> Q BioMed ... provide the following update on recent corporate developments. ... months we have significantly increased our cash position through several ... result, we have positioned ourselves to execute on the initial ... that development to continue on schedule. --> ...
Breaking Biology Technology: