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'No muss, no fuss' miniaturized analysis for complex samples developed
Date:11/18/2009

a buffer solution under controlled pressure in the opposite direction. This opposing pressure flow acts as a "fluid gate" between the sample reservoir and the microchannel. Gradually reducing the pressure of the counterflow opens the "gate" a little bit at a time. A specific sample component is detected when the pressure flow becomes weak enoughwhen the "gate" opens wide enoughthat the component's electrophoretic motion pushes it against the pressure flow and into the channel for detection. In this way, different components enter the channel at different times based on their particular electrophoretic motion. Most importantly, the channel doesn't become fouled because the unwanted material in the sample is held out during the analysis by the pressure flow.

In their paper, the researchers validated their GEMBE analysis technique by testing it with solutions of whole milk, dirt, estuarine sediment, coal fly ash, pulverized leaves and blood serum. In all casesand without the muss and fuss of pre-analysis sample preparationthe system was able to reproducibly separate and quantify specific components from the solutions, including potassium, calcium, sodium, magnesium, lithium and melamine.

"GEMBE is well-suited to the microfluidic analysis of 'real-world' samples," Strychalski says. "We have shown that the method can handle solutions containing particulates, proteins and other materials that would confound the majority of other microfluidic techniques."

Because of its ability to easily and rapidly characterize complex mixtures with minimal preparation, the researchers believe that GEMBE shows enormous promise for diverse applications, such as monitoring contaminants in food or water supplies, determining nutrient levels in soil, detecting biochemical warfare agents, and diagnosing medical conditions. The next steps, they say, are to miniaturize the desktop equipment now used in the system and integrate all of the parts to develop a true "lab-on
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Contact: Michael E. Newman
michael.newman@nist.gov
301-975-3025
National Institute of Standards and Technology (NIST)
Source:Eurekalert  

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