Navigation Links
No extra mutations in modified stem cells, study finds
Date:7/9/2014

LA JOLLA-The ability to switch out one gene for another in a line of living stem cells has only crossed from science fiction to reality within this decade. As with any new technology, it brings with it both promise--the hope of fixing disease-causing genes in humans, for example--as well as questions and safety concerns. Now, Salk scientists have put one of those concerns to rest: using gene-editing techniques on stem cells doesn't increase the overall occurrence of mutations in the cells. The new results were published July 3 in the journal Cell Stem Cell.

"The ability to precisely modify the DNA of stem cells has greatly accelerated research on human diseases and cell therapy," says senior author Juan Carlos Izpisua Belmonte, professor in Salk's Gene Expression Laboratory. "To successfully translate this technology into the clinic, we first need to scrutinize the safety of these modified stem cells, such as their genome stability and mutational load."

When scientists want to change the sequence of a stretch of DNA inside cells--either for research purposes or to fix a genetic mutation for therapeutic purposes--they have their choice of two methods. They can use an engineered virus to deliver the new gene to a cell; the cell then integrates the new DNA sequence in place of the old one. Or scientists can use what's known as custom targeted nucleases, such as TALEN proteins, which cut DNA at any desired location. Researchers can use the proteins to cut a gene they want to replace, then add a new gene to the mix. The cell's natural repair mechanisms will paste the new gene in place.

Previously, Belmonte's lab had pioneered the use of modified viruses, called helper-dependent adenoviral vectors (HDAdVs) to correct the gene mutation that causes sickle cell disease, one of the most severe blood diseases in the world. He and his collaborators used HDAdVs to replace the mutated gene in a line of stem cells with a mutant-free version,
'/>"/>

Contact: Kristina Grifantini
press@salk.edu
Salk Institute
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Chinese herbal extract may help kill off pancreatic cancer cells
2. NREL finds up to 6-cent per kilowatt-hour extra value with concentrated solar power
3. Grape skin extract may soon be answer to treating diabetes
4. Are we ready for contact with extraterrestrial intelligence?
5. Search for extraterrestrial life more difficult than thought
6. Trisomy 21: How an extra little chromosome throws the entire genome off balance
7. Plant extract offers hope for infant motor neuron therapy
8. Garcinia Cambogia Extract Review Company Garcinia Ex Announces the Launch of its Updated Website
9. A magnetic nanoparticles-based method for DNA extraction from the saliva after stroke
10. Wayne State cholesterol study shows algal extracts may counter effects of high fat diets
11. A universal RNA extraction protocol for land plants
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
No extra mutations in modified stem cells, study finds
(Date:9/30/2014)... 30, 2014 Winners of The Economist,s prestigious ... scheduled to take place at the JW Marriott Hong Kong on ... winners will share their experiences and the lessons those hold for ... proven innovation over the past decade, will be presented at a ... the summit. It will be the first time the ceremony has ...
(Date:9/29/2014)... study confirms that the exposure to tar tended to ... regular cigarettes. Similarly, exposure to nicotine tended to ... type of cigarette in several countries around the world. ... toxic chemicals in the smoke of these cigarettes are ... levels of chemicals in the smoke are not necessarily ...
(Date:9/29/2014)... 1970 and 2010 populations of mammals, birds, reptiles, ... percent, says the 2014 Living Planet Report released ... loss occurs disproportionately in low-income countriesand correlates with ... In addition to the precipitous decline in wildlife ... signs about the overall health of the planet. ...
Breaking Biology News(10 mins):Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 2Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 3Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 4Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 5Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 6Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 7Slim cigarette smokers not exposed to more harmful chemicals 2Half of global wildlife lost, says new WWF report 2
... 10, 2009 The year 2009 marks the 150 ... the Species " a seminal book that permeated ... marks the bicentennial anniversary of Darwin,s birth, the following ... Father of Evolution influenced biology to anthropology and philosophy ...
... higher elevations in Arizona,s Santa Catalina Mountains as summer ... of Arizona in Tucson. The flowering ranges of ... compared to where the same species flowered the previous ... in the region increased about 1.8 degree Fahrenheit (1 ...
... coral reefs isn,t simply caused by too many people, according ... of Current Biology , a Cell Press publication. Rather, ... coral reefs in the western Indian Ocean depended mostly on ... of the people living on the shores., Specifically, they found, ...
Cached Biology News:Darwin's seminal impact on biology, anthropology, philosophy and psychology 2Darwin's seminal impact on biology, anthropology, philosophy and psychology 3Darwin's seminal impact on biology, anthropology, philosophy and psychology 4Plants take a hike as temperatures rise 2Plants take a hike as temperatures rise 3Rise or fall of reef fish driven by both economy and ecology 2
(Date:10/1/2014)... TOLEDO’s newly improved XS balance line includes analytical ... a dual range microbalance . As a revolutionary ... delivers results up to twice as fast, even in ... mean users no longer need to suffer the stresses ... of the balance. Barbara Bienlein, medical specialist and managing ...
(Date:10/1/2014)... (PRWEB) October 01, 2014 Weighing ... quality of the final product. However, while the ... regulatory frameworks such as Good Manufacturing Practices (GMP) ... responsibility of the manufacturer to interpret these guidelines ... weighing performance quality. METTLER TOLEDO experts have created ...
(Date:10/1/2014)... City, CA (PRWEB) October 01, 2014 As ... it leaves no room for doubt that this century would ... innovations in the field of medicine science. The fact that ... problems is not unknown to the scientific community. Almost half ... pollution and therefore can be prevented with medical care and ...
(Date:10/1/2014)... 2014 In response to its ... address sales, training and consumer engagement challenges, ... mobile applications to turn their clients’ visions into ... customized mobile app creations, are now featured on ... partnership with Toyota, StudioPMG created a robust cross-platform ...
Breaking Biology Technology:METTLER TOLEDO’s XS Balance Line Delivers Performance and Ergonomics To Safeguard Health in the Workplace 2METTLER TOLEDO’s XS Balance Line Delivers Performance and Ergonomics To Safeguard Health in the Workplace 3Comply with Weighing-Related Regulations in Pharma Manufacturing 2Fighting Mortality and Morbidity of Chronic Health Disorders during Open Access Week 2Fighting Mortality and Morbidity of Chronic Health Disorders during Open Access Week 3Digital Agency Studio PMG Launches New Case Studies on Website 2
... drives technology. Stop worrying about whether or not technology ... technology is to that technology. In technology, as with love, ... you probably already know and dont like the answer. ... of your technological horsepower is actually being used to turn ...
... are their own worst enemies when it comes to retaining young ... that have given up on this task. Many seem to believe ... phobia that prevents them from staying in any one company for ... are undoubtedly some young people who have ants in their pants, ...
... - Agilent Technologies has announced a new ... of one gigabit per second, using designs bought from ... a firm based in Eau Claire, designs application-specific integrated ... of its implementation of System Packet Interface Level 4 ...
Cached Biology Technology:Technology as the engine of business innovation 2Technology as the engine of business innovation 3How to keep young talent working for you 2How to keep young talent working for you 3Agilent's new network chip was designed in Wisconsin 2
PKC (pSer345), phospho-specific...
PE Rat anti-Hu CD267 Storage Temperature: Refrigerate(2 to 8C)...
Mouse monoclonal [4i144] to HLA Class II DQ1 7 ( Abpromise for all tested applications). Antigen: Full length protein (Human): HLA Class 2 Antigen DQ1,7....
ASCIZ...
Biology Products: