Navigation Links
Next generation microscopy: No stain, big gain
Date:12/18/2008

Microscopes have revolutionized the practice of science, especially in the fields of biology and medicine. Just a few hundred years ago, gaining the ability to study what was previously unobservable opened up an entirely new world. Today, imaging techniques remain indispensable to clinicians and researchers who regularly diagnose medical conditions and work to develop new treatments.

Test results can often take hours or even days because cells or tissues must be subjected to lengthy fixation and labeling processes, sometimes called staining, in order to visualize and distinguish cellular components. In addition to long processing times, staining procedures often include harsh treatments or conditions that alter the tissues themselves, making interpretation of results difficult.

A newly developed label-free imaging technique called stimulated Raman scattering (SRS) will likely revolutionize biomedical imaging in research and diagnostic laboratories. A team lead by Sunney Xie at Harvard University reported this new technique in the December 19 issue of Science.

"It is a big step forward in terms of biology," said Xie. "SRS is a powerful imaging modality with widespread applications on many fronts of biology and medicine. This work compliments an earlier technique we developed with funding from the National Science Foundation, adding a new imaging modality to the vibrational microscopy field."

The key to this new chemical imaging technique is the use of two lasers with different frequencies. Researchers visualize samples by tuning the laser frequencies to match the vibrational frequency of a specific chemical bond. Each type of molecule within a sample, including nutrients or drugs, is detectable at a unique frequency. By combining sample data collected at numerous frequencies, researchers can produce a high-resolution 3D image of the sample. SRS microscopy represents a big gain in biomedical imaging because it avoids labor-intensive sample preparation and autofluorescence, or "background noise", associated with traditional fluorescence microscopy.

Xie is enthusiastic about the ways in which SRS imaging will facilitate progress in many fields. "Applications of SRS imaging range from mapping distribution of small metabolite and drug molecules in cells and tissues to medical diagnosis of cancer. Neuroimaging is another exciting area of application."


'/>"/>

Contact: Lisa Van Pay
lvanpay@nsf.gov
703-292-8796
National Science Foundation
Source:Eurekalert

Related biology news :

1. e-Smart Technologies to Introduce Next Generation I AM Smart Card Technology at Cartes 2008 in Paris
2. Gene therapy restores vision to mice with retinal degeneration
3. Cholesterol-lowering drugs and the effect on muscle repair and regeneration
4. Generation innovation: Young UC San Diego bioengineer to use NIH grant to fuel tissue engineering
5. ESA trains next generation of atmospheric scientists
6. Entrust Expands Relationship with Slovenia, Implements Second-generation ePassport Solution
7. Rhode Island Hospital study finds link between obesity, type 2 diabetes and neurodegeneration
8. Songbirds may hold key to advances in treatment of brain degeneration
9. Broad Institute researchers introduce next generation tool for visualizing genomic data
10. The epigenetics of increasing weight through the generations
11. Study shows single insecticide application can kill 3 cockroach generations
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/23/2017)... Research and Markets has announced the addition ... - Industry Forecast to 2025" report to their offering. ... The Global Vehicle ... around 8.8% over the next decade to reach approximately $14.21 billion ... estimates and forecasts for all the given segments on global as ...
(Date:3/22/2017)... LIVERMORE, Calif. , March 21, 2017 ... recognition analytics company serving law enforcement agencies, announced today ... Sheridan as director of public safety business development. ... of diversified law enforcement experience, including a focus on ... Vigilant. In his most recent position, Mr. Sheridan served ...
(Date:3/16/2017)... Germany , March 16, 2017 CeBIT 2017 - Against identity ... Continue Reading ... Used combined in one project, multi-biometric solutions provide ... Used ... Systems) ...
Breaking Biology News(10 mins):
(Date:5/24/2017)... ... May 24, 2017 , ... ... as treasurer for the Mid-Atlantic chapter of the Healthcare Businesswomen’s Association ... , The HBA Mid-Atlantic chapter board meets in person once each quarter and ...
(Date:5/24/2017)... ... May 23, 2017 , ... ... permanent modular buildings, announced the launch of the Mobile Big Room. This ... to help support on-site teamwork and collaboration. , The Mobile Big Room ...
(Date:5/24/2017)... ... May 24, 2017 , ... Patient Monitoring ... with Wi-Fi connectivity to reduce the amount of wiring in a healthcare facility ... compact mobile devices including infusion pumps, heart and hypertension monitoring, glucose monitoring, and ...
(Date:5/23/2017)... ... May 23, 2017 , ... ... cloud-based file transfer solution that makes it easy for organizations to send and ... worry about cumbersome FTP software or email file size limitations. , Using ...
Breaking Biology Technology: