Navigation Links
Newly found DNA catalysts cleave DNA with water molecule
Date:8/16/2009

CHAMPAIGN, Ill. Better tools for manipulating DNA in the laboratory may soon be possible with newly discovered deoxyribozymes (catalytic DNA) capable of cleaving single-stranded DNA, researchers at the University of Illinois say.

The deoxyribozymes accomplish the DNA cleavage with the sequence-selectivity and site-selectivity required for a practical catalyst, the researchers say.

"Our work suggests that deoxyribozymes have significant potential as sequence-specific DNA cleavage reagents," said chemistry professor Scott Silverman. "The hope is that we can take this fundamental advance and develop the ability to use DNA as a practical catalyst to cleave double-stranded DNA."

Silverman, postdoctoral research associate Madhavaiah Chandra and graduate student Amit Sachdeva report their discovery in a paper accepted for publication in Nature Chemical Biology and posted on the journal's Web site.

The researchers discovered the new deoxyribozymes while searching for artificial sequences of DNA that could cleave proteins. The newly found catalysts function in a fashion similar to restriction enzymes, although to date by cleaving only single-stranded DNA.

Restriction enzymes, which allow scientists to cut and paste portions of double-stranded DNA, are the fundamental catalysts of molecular biology.

Each restriction enzyme, however, has a limited number of DNA sequences it can cut. Consequently, only a few percent of arbitrarily chosen DNA sequences can be cut by commercially available restriction enzymes.

Like natural restriction enzymes, the new catalysts are both sequence-specific and site-specific. "This means we can target a particular sequence, and we know we will cut at only one site within that sequence," Silverman said. "By appropriately picking the recognition and enzyme regions of the catalyst, we should be able to cut many more DNA sequences than is possible with current restriction enzymes."

The new DNA catalysts require two metal ions manganese and zinc to carry out their catalysis, "which is intriguing, because many natural protein-based nucleases (which cleave DNA) similarly require two metal ions," Silverman said. "One or both of the metals are presumably involved in the chemical mechanism by which our DNA catalyst achieves hydrolysis of the DNA backbone."

DNA hydrolysis is a very challenging chemical reaction, much more difficult to perform than the cleavage of a strand of RNA, Silverman said. In cleaving DNA, a water molecule must be brought in for the breaking reaction to occur. Also, both the DNA and the catalyst must be arranged appropriately in three-dimensional space.

How all of this happens with the DNA catalysts is not yet clear. Silverman's research group continues to probe the structure and mechanism of the catalysts, along with identifying and characterizing catalysts with different recognition sites.

"So far, we have achieved cleavage of single-stranded DNA targets," Silverman said. "The next big step is to cleave double-stranded DNA targets."


'/>"/>

Contact: James E. Kloeppel
kloeppel@illinois.edu
217-244-1073
University of Illinois at Urbana-Champaign
Source:Eurekalert

Related biology news :

1. Newly discovered gene fusion may lead to improved prostate cancer diagnosis
2. Newly discovered snow roots are evolutionary phenomenon
3. Newly discovered reactions from an old drug may lead to new antibiotics
4. Newly discovered epidermal growth factor receptor active in human pancreatic cancers
5. Newly discovered gene plays vital role in cancer
6. DNA evidence is in, newly discovered species of fish dubbed H. psychedelica
7. Newly described contaminant sources in Katrina-flooded homes pose health risks
8. UC Davis research shows that newly discovered drug reduces heart enlargement
9. Obesity starts in the head? 6 newly discovered genes for obesity have a neural effect
10. Newly found enzymes may play early role in cancer
11. Newly identified gene powerful predictor of colon cancer metastasis
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/24/2016)... Ampronix facilitates superior patient care by providing unparalleled technology to leaders of ... the latest premium product recently added to the range of products distributed by Ampronix. ... ... ... Medical Display- Ampronix News ...
(Date:5/3/2016)... , May 3, 2016  Neurotechnology, a ... the MegaMatcher Automated Biometric Identification System (ABIS) ... large-scale multi-biometric projects. MegaMatcher ABIS can process multiple ... using any combination of fingerprint, face or iris ... MegaMatcher SDK and MegaMatcher Accelerator , ...
(Date:4/19/2016)... The new GEZE SecuLogic access ... "all-in-one" system solution for all door components. It can ... door interface with integration authorization management system, and thus ... minimal dimensions of the access control and the optimum ... offer considerable freedom of design with regard to the ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... June 23, 2016 , ... Charm Sciences, Inc. ... test has received AOAC Research Institute approval 061601. , “This is another AOAC-RI ... stated Bob Salter, Vice President of Regulatory and Industrial Affairs. “The Peel Plate ...
(Date:6/23/2016)... ON (PRWEB) , ... June 23, 2016 , ... STACS ... DNA Technical Leader at the Arkansas State Crime Laboratory, has joined STACS DNA as ... the STACS DNA team,” said Jocelyn Tremblay, President and COO of STACS DNA. “In ...
(Date:6/23/2016)... On Wednesday, June 22, 2016, the NASDAQ Composite ... Jones Industrial Average edged 0.27% lower to finish at 17,780.83; ... has initiated coverage on the following equities: Infinity Pharmaceuticals Inc. ... NKTR ), Aralez Pharmaceuticals Inc. (NASDAQ: ARLZ ), ... more about these stocks by accessing their free trade alerts ...
(Date:6/23/2016)... 23, 2016 ReportsnReports.com adds ... to its pharmaceuticals section with historic and forecast ... much more. Complete report on the ... profiling 15 companies and supported with 261 tables ... . The Global Cell Culture ...
Breaking Biology Technology: