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Newly developed medium may be useful for human health, biofuel production, more
Date:7/3/2013

COLLEGE STATION Texas A&M University System scientists from the departments of nutrition and food science and poultry science have developed a new medium for the cultivation of beneficial microorganisms called lactobacilli.

A better understanding of lactobacilli metabolism can help improve feed efficiency in animals and combat malnutrition in humans, according to the researchers.

"Lactobacilli are normal residents of the human gastrointestinal and urogenital tracts, where they promote host health and can be taken as probiotics," said Dr. Joseph Sturino, a Texas A&M AgriLife Research assistant professor in the department of nutrition and food science, Texas A&M's College of Agriculture and Life Sciences, and lead investigator for the study.

Other lactobacilli are used to manufacture fermented food products and to drive the bioconversion of waste streams into value-added products, such as biofuels and prebiotics.

"Unfortunately, the nutrient compositions of media that are traditionally used to cultivate lactobacilli are largely undefined," said Dr. Rani Menon, an AgriLife Research postdoctoral research associate who conducted this research.

This lack of definition complicates their use when trying to identify nutrients to stimulate the growth and metabolic activity of these important microorganisms.

To address this need, Sturino and Menon, along with undergraduate researcher Meredith Shields and assistant professor of poultry science Dr. Tri Duong, set out to develop a better-defined medium for use in metabolic screens.

"We devised a series of single-component omission experiments to determine which undefined ingredients found in the current 'gold standard' medium could be eliminated or replaced," he explained. "Concentration titration experiments were then used to fine-tune the final formulation in order to support the growth of a benchmark species of interest."

As a result of these expe
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Contact: Dr. Joseph Sturino
joseph.sturino@tamu.edu
979-458-1850
Texas A&M AgriLife Communications
Source:Eurekalert

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NM522 contains an F episome, which is required for production of ssDNA and for blue/white color selection. To maintain the F, NM522 should be grown on minimal (M-9) medium....
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