Navigation Links
New virtual tool may provide more accurate diagnosis of genetic mutations
Date:2/5/2012

Philadelphia, PA, February 6, 2012 DNA sequencing to detect genetic mutations can aid in the diagnosis and selection of treatment for cancer. Current methods of testing DNA samples, Sanger sequencing and pyrosequencing, occasionally produce complex results that can be difficult or impossible to interpret. Scientists at the Johns Hopkins University School of Medicine have developed a free software program, Pyromaker, that can more accurately identify such complex genetic mutations.

Pyromaker is a web-based application that produces simulated pyrograms based on user input including the percentage of tumor and normal cells, the wild-type sequence, the dispensation order, and any number of mutant sequences. Pyromaker calculates the relative mutant and wild-type allele percentages and then uses these to generate the expected signal at each point in the dispensation sequence. The final result is a virtual trace of the expected pyrogram.

The researchers validated Pyromaker against actual pyrograms containing common mutations in the KRAS gene, which plays an important role in the pathogenesis of a variety of tumors. The actual pyrograms and virtual pyrograms were quantitatively identical for all mutations tested.

They then demonstrated that all codon 12 and 13 single and complex mutations generate unique pyrograms. However, some complex mutations were indistinguishable from single base mutations, indicating that complex mutations may be underreported. Working with two complex pyrograms that were difficult to interpret initially, the researchers identified five approaches to resolve them: Sanger sequencing alone, hypothesis testing with Pyromaker, Pyromaker iterative mutation re-creation, melting curve analysis, and TA cloning with Sanger sequencing.

Senior author James R. Eshleman, MD, PhD, Professor of Pathology and Oncology, Associate Director, Molecular Diagnostics Laboratory, Johns Hopkins University School of Medicine
'/>"/>

Contact: David Sampson
jmdmedia@elsevier.com
215-239-3171
Elsevier Health Sciences
Source:Eurekalert

Page: 1 2

Related biology news :

1. £20 million to fight virtual crime and treat our ageing population
2. £20 million to fight virtual crime and treat our aging population
3. Virtual reality: Keyhole surgeons training could help meet European working time directives
4. A virtual physicians conference
5. ASCB wins NIH stimulus grant to build virtual library of cell images for researchers and public
6. CSI in a virtual world: New grant furthers NC States work in forensic science
7. A virtual liver, a better chance of life
8. Glencoe Software to Support the ASCB Stimulus Grant to Build Virtual Image Library of the Cell
9. Penn bioengineers create simulator to test blood platelets in virtual heart attacks
10. Designing touch-sensitive virtual reality tools to train and test tomorrows surgeons
11. Wiecha receives grant to study face-to-face vs. virtual health education for African-American women
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/31/2014)... Two new meta-analyses involving tree nuts (almonds, Brazil nuts, ... were recently published in the online publications, British ... PLOS ONE (ii). The BMJ Open ... metabolic syndrome (MetS) criteria and showed that tree nut ... fasting blood glucose. The PLOS ONE article ...
(Date:7/30/2014)... Queen in Lewis Carroll,s Through the Looking Glass, ... University of Singapore improved a 35-year-old ecology model ... to millions of years. , The new model, ... the "Red Queen Effect," an evolutionary hypothesis introduced ... suggests that organisms must constantly increase their fitness ...
(Date:7/30/2014)... climate change on the world,s animal and plant ... to a new paper by a research team ... and other organizations. The authors claim that most ... when it comes to climate change. , ... on the "direct" threats of changing temperatures and ...
Breaking Biology News(10 mins):New international tree nut council study looks at nuts, diabetes and metabolic syndrome 2Classic Lewis Carroll character inspires new ecological model 2Classic Lewis Carroll character inspires new ecological model 3Conservation scientists asking wrong questions on climate change impacts on wildlife 2
... Old muscle got a shot of youthful vigor in a ... Berkeley, setting the path for research on new treatments for ... Parkinson,s diseases. In a new study to be published ... Nature , researchers identified two key regulatory pathways that ...
... The University of Delaware has received $400,000 from the ... of an ocean observing system for the Mid-Atlantic region, ... Cod, Mass., and out to the continental shelf. Nearly ... Mid-Atlantic coast. The initiative is part of the ...
... Pep Canadell from CSIRO and the Global Carbon Project, ... billions of tons of carbon dioxide (CO2) absorbed annually ... climate change mitigation worth hundreds of billions of dollars. ... stocks, challenges in quantifying changes in the size of ...
Cached Biology News:Stem cell researchers give old muscle new pep 2Stem cell researchers give old muscle new pep 3Stem cell researchers give old muscle new pep 4UD nets NOAA funding for Mid-Atlantic ocean observing 2Tropical forest sustainability: A climate change boon 2
(Date:7/31/2014)... 2014 Today BioSpace , the leading life sciences employment ... spotlighting the life sciences community in Illinois , ... , Michigan , Minnesota , ... Ohio and Wisconsin . BioMidwest ... all job postings on BioSpace originating in this region in 2013. The ...
(Date:7/31/2014)... the sound-processing part of the mouse brain in a ... trees in view. Their imaging technique allows zooming in ... and it enabled the team to watch brain cells ... results, which represent a step toward better understanding how ... the journal Neuron . , In the past, ...
(Date:7/31/2014)... Graphite Metallizing Corporation announces GRAPHALLOY® bearings ... are uniquely suited to prevent loss of lubrication that ... high temperatures . , Recently, a manufacturer of ... at Graphite Metallizing to overcome problems with movement of ... pans containing blocks of powder mixture are loaded onto ...
(Date:7/31/2014)... Senomyx , Inc. (NASDAQ: ... taste science technologies to discover, develop, and commercialize ... ingredient supply industries, today reported financial results for ... second quarter with $33.6 million in cash and ... past quarter Senomyx achieved another important milestone with ...
Breaking Biology Technology:Midwest Biotech Leaders Featured On BioSpace Map 2New mapping approach lets scientists zoom in and out as the brain processes sound 2Graphite Metallizing Announces Bearings for High Temperature Oven Applications 2Graphite Metallizing Announces Bearings for High Temperature Oven Applications 3SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 2SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 3SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 4SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 5SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 6SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 7SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 8SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 9SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 10SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 11SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 12SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 13SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 14SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 15SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 16
... pET vectors for bacterial expression has been the cost ... In order to tackle this problem, DNA2.0 has developed ... free of IP constraints. pJexpress vectors are specifically engineered ... shown to express better than comparable pET vectors. pJexpress ...
... US Oncology, Inc ., one of the nation,s largest cancer care ... Most Admired Companies list for 2009. The company ranks third ... third time US Oncology has been honored with F ... named to the list in 2004 and 2007 in ...
... a leading provider of centralized ECG and eClinical technology, ... and related industries, announced today that it will be ... #1253/1254 at the 21st Annual DIA Euromeeting, 23-25 March ... to three speaking sessions, which will discuss the impact ...
Cached Biology Technology:DNA2.0 Introduces Freedom of Expression With a New Family of Bacterial Expression Vect:ors 2DNA2.0 Introduces Freedom of Expression With a New Family of Bacterial Expression Vect:ors 3US Oncology Named to FORTUNE Magazine's Most Admired Companies List for Third Time 2US Oncology Named to FORTUNE Magazine's Most Admired Companies List for Third Time 3ERT to Showcase Its Clinical Trials Portfolio at DIA's 21st Euromeeting 2ERT to Showcase Its Clinical Trials Portfolio at DIA's 21st Euromeeting 3
... Detection Platform combines robust chemistry with ... sensitive, specific, and cost-effective detection method ... Platform offers exceptional performance with specificity ... high signal/background ratio, and an expanded ...
Request Info...
... Gel Band Purification Kit, 100. For the purification, ... fragments (0.1-10 kb) from solution and from TAE ... from solution in as little as 5 min ... min.Prepacked glass fiber matrix columns provide consistent results ...
BD BaculoGold Max-XP Insect Cell Medium 1 Liter...
Biology Products: